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Biomedical subjects

K MacLean

Publications and source records attributed to K MacLean.

4 recordsLinked to original sources
PubMed2000

A simple sequence repeat-based linkage map of barley.

A total of 568 new simple sequence repeat (SSR)-based markers for barley have been developed from a combination of database sequences and small insert genomic libraries enriched for a range of short simple sequence repeats. Analysis of the SSRs on 16 barley cultivars revealed variable levels of informativeness but no obvious correlation was found with SSR repeat length, motif type, or map position. Of the 568 SSRs developed, 242 were genetically mapped, 216 with 37 previously published SSRs in a single doubled-haploid population derived from the F(1) of an interspecific cross between the cultivar Lina and Hordeum spontaneum Canada Park and 26 SSRs in two other mapping populations. A total of 27 SSRs amplified multiple loci. Centromeric clustering of markers was observed in the main mapping population; however, the clustering severity was reduced in intraspecific crosses, supporting the notion that the observed marker distribution was largely a genetical effect. The mapped SSRs provide a framework for rapidly assigning chromosomal designations and polarity in future mapping programs in barley and a convenient alternative to RFLP for aligning information derived from different populations. A list of the 242 primer pairs that amplify mapped SSRs from total barley genomic DNA is presented.

Chromosome Mapping↗
PubMed1995

Nursing criteria for patient progression post myocardial infarction.

Nurses play a pivotal role in the evaluation of patients with coronary artery disease, since many symptoms are subtle and arise while carrying out activities. Consistency among nursing staff in interpreting patient readiness for activity is crucial to ensure that patients are progressing steadily or to determine if a delay in their progression is warranted. While there have been studies on patient reaction to myocardial infarction and on the physiological effects of activity and/or nursing interventions on myocardial patients, no studies could be found that have examined the criteria used by nurses to assess when patients are ready to increase, decrease or maintain a level of activity according to specific activity protocols. This descriptive study examined the criteria utilized by experienced staff nurses in determining post myocardial patients' readiness to progress through the institution's designated activity levels. The results indicated that in-depth criteria were utilized by these experienced nurses and that patient situations existed whereby it was difficult to determine patient readiness. Factors were discovered that were perceived to enhance or inhibit patient's progression through designated activity levels. Recommendations from this study support the need to (a) examine the use of current activity protocols for their utility in nursing decision making, (b) consider the experiential base of practicing nurses as well as the working environment in any further development of activity protocols.

Activities of Daily Living↗
PubMed1988

Six-month treatment outcomes in socially stable alcoholics: abstinence rates.

A randomly selected sample of 181 socially stable patients were followed for a period of six months after leaving alcoholism treatment. Collateral verification of drinking status was used to validate patient self-reports. A follow-up rate of 94% was achieved. For all patients, a continuous abstention rate (no drinking at all for the entire six-month period) of 61% was achieved, while 72% of the located patients were currently abstinent at the time of followup. For alcoholics with no other drug problems, a 66% continuous abstention rate was achieved, and 77% were currently abstinent at followup. These results suggest that alcoholism treatment can be effective for samples of socially stable alcoholics treated with multimodal treatments in a specialized, freestanding, alcoholism treatment facility.

Adult↗