Chronic idiopathic myelofibrosis expressing a novel type of TEL-PDGFRB chimaera responded to imatinib mesylate therapy.
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Adult↗
Biomedical subjects
K Maki
Publications and source records attributed to K Maki.
Development of megakaryoblastic leukaemia in Runx1-Evi1 knock-in chimaeric mouse.
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Animals↗
Pressure dependence of the Fulde-Ferrell-Larkin-Ovchinnikov state in CeCoIn5.
Pressure studies of the thermodynamics of CeCoIn5 under magnetic fields H parallel to c and H parallel to ab have been made up to P = 1.34 GPa. We recorded the signature of the Fulde-Ferrell-Larkin-Ovchinnikov (FFLO) state for all pressures when H parallel to ab. Also remarkably, the FFLO regime suddenly expands for P = 1.34 GPa. With the help of a microscopic theory for d-wave superconductivity, we have extracted the gyromagnetic ratio g and the Fermi velocities nu(a) and nu(c). Our study is the first evidence for the existence of the FFLO state away from the influence of the antiferromagnetic fluctuations. We find a close parallel between the T-P phase diagram of CeCoIn5 and the T-x phase diagram of the high-Tc cuprates, where x is the hole concentration.
Journal Article↗
Overexpression of Candida albicans CDR1, CDR2, or MDR1 does not produce significant changes in echinocandin susceptibility.
The micafungin and caspofungin susceptibilities of Candida albicans laboratory and clinical isolates and of Saccharomyces cerevisiae strains stably hyperexpressing fungal ATP-binding cassette (ABC) or major facilitator superfamily (MFS) transporters involved in azole resistance were determined using three separate methods. Yeast strains hyperexpressing individual alleles of ABC transporters or an MFS transporter from C. albicans gave the expected resistance profiles for the azoles fluconazole, itraconazole, and voriconazole. The strains hyperexpressing CDR2 showed slightly decreased susceptibility to caspofungin in agar plate drug resistance assays, as previously reported, but increased susceptibility to micafungin compared with either the strains hyperexpressing CDR1 or the null parent deleted of seven ABC transporters. The strains hyperexpressing CDR1 showed slightly decreased susceptibility to micafungin in these assays. A C. albicans clinical isolate overexpressing both Cdr1p and Cdr2p relative to its azole-sensitive isogenic progenitor acquired resistance to azole drugs and showed reduced susceptibility to caspofungin and slightly increased susceptibility to micafungin in agar plate drug resistance assays. None of the strains showed significant resistance to micafungin or caspofungin in liquid microdilution susceptibility assays. The antifungal activities of micafungin and caspofungin were similar in agarose diffusion assays, although the shape and size of the caspofungin inhibitory zones were affected by medium composition. The assessment of micafungin and caspofungin potency is therefore assay dependent; the differences seen with agar plate drug resistance assays occur over narrow ranges of echinocandin concentrations and are not of clinical significance.
ATP-Binding Cassette Transporters↗
Root fracture in immature anterior teeth followed for 15 years.
We report a case of injury to an immature tooth, observed over a period of 15 years. In 1987, a 9-year-old boy fell down in a schoolyard. The right central incisor demonstrated palato-version and radiographic observations revealed that the roots of both central incisors were incomplete. Further, a root fracture in the apical region of the central incisors was observed. During the first treatment visit, the right central incisor was repositioned and both teeth splinted. After confirming that the line of fracture was aligned, the fixation was continued for 2 months. The teeth were examined periodically for the next 15 years. Both teeth had favorable outcomes with continued root development of both the apical and coronal segments with good apposition of the fracture lines.
Child↗
Genome-wide linkage analysis of mandibular prognathism in Korean and Japanese patients.
The existence of familial aggregation of mandibular prognathism (MP) suggests that genetic components play an important role in its etiology. In this study, a genome-wide linkage analysis to identify loci susceptible to MP was conducted with 90 affected sibling-pairs in 42 families, comprised of 40 Korean sibling-pairs and 50 Japanese sibling-pairs. Two non-parametric linkage analyses, GENEHUNTER-PLUS and SIBPAL, were applied and detected nominal statistical significance of linkage to MP at chromosomes 1p36, 6q25, and 19p13.2. The best evidence of linkage was detected near D1S234 (maximum Z(lr) = 2.51, P = 0.0012). In addition, evidence of linkage was observed near D6S305 (maximum Z(lr) = 2.23, P = 0.025) and D19S884 (maximum Z(lr) = 1.93, P = 0.0089). Identification of the susceptible genes in the linkage regions will pave the way for insights into the molecular pathways that cause MP, especially overgrowth of the mandible, and may lead to the development of novel therapeutic tools.
Adolescent↗
Eruption sequestrum: x-ray microanalysis and microscopic findings.
A 12 year-old girl with eruption sequestrum of the mandibular left second molars is reported. Intraoral findings revealed that a small hard tissue fragment white in color and with bone-like hardness on the occlusal surface of the mandibular second molar which was erupting. Histopathologically, the fragments consisted of necrotized cortical bone. Chronic inflammatory alterations were also observed in the gingiva in the area of contact with the osseous tissue. X-ray microanalyzer findings revealed the percentages of calcium and phosphorous (by weight) as 78.41% and 21.59%, respectively, for a calcium to phosphorous ratio of 3.63, which was higher than that seen in normal osseous tissue.
Bone and Bones↗
[Diaphragmatic eventration resulting from phrenicectomy treated with surgical method; report of a case].
The case was a 59-year-old man who has a history of left mediastinal tumor resection with left phrenicectomy. The elevated diaphragm revealed by chest X-ray 7 years after the operation led to diagnosis of diaphragmatic eventration. Since any symptom was seen in the early period, "wait and watch" strategy was done for management. Both the abdominal enlarged feeling and the dyspnea on effort were appeared 10 years after the operation. Under the speculation of these symptoms related to the elevated abdominal organs came up with diaphragmatic eventration, surgical method the plication of the diaphragm was performed. The diaphragm was plicated by interrupted suture as opening the diaphragm to avoid injury the abdominal organs, and reinforced with the Marlex mesh. We used artificial mesh to reinforce the thin diaphragm with exceptation of prevent the postoperative recurrence, because a result of the etiological process of the case was considered as disuse atrophy of diaphragm after phrenicectomy.
Diaphragm↗
Mechanical assessment of effects of grape seed proanthocyanidins extract on tibial bone diaphysis in rats.
We studied the effects of grape seed proanthocyanidins extract (GSPE) given as a ratio of 3 mg in 100 g in a standard diet, on the tibial bone diaphysis in low-calcium fed rats. Measurements of bone density, mineral content, geometry, and bone strength using peripheral quantitative computed tomography (pQCT). Further, the whole tibia bones were tested for mechanical resistance using a material-testing machine, and mineral elements were also determined. Forty male Wistar rats, 5 weeks old, were divided into control (Co), low-calcium diet (LC), low-calcium diet . standard diet (LCS), and low-calcium diet . standard diet with supplementary GSPE (LCSG) groups. We found no significant differences in body weight among the 4 groups, whereas all of the bone parameters in LC were significantly lower than those in Co (p<0.01, except in periosteal perimeter (Peri) p<0.05). The cortical bone mineral content (CtBMC), cortical bone density (CtvBMD) and Peri in LCSG were significantly higher than those in LCS (p<0.01; p<0.01; p<0.05, respectively). All bone parameters in LCSG were significantly higher than those in LC (p<0.01, except in Peri, and stress strain index to reference axis x (xSSI) p<0.05)). In addition, Ca, P, and Zn contents in LCSG were significantly higher than those in LCS (p<0.01; p<0.01; p<0.05, respectively). Our results suggest that GSPE included in a diet mixture with calcium has a beneficial effect on bone formation and bone strength for the treatment of bone debility caused by a low level of calcium.
Animals↗
[Primary pulmonary meningioma; report of a case].
Primary pulmonary meningiomas are quite rare, and their occurrence has been reported only sporadically. A 49-year-old, asymptomatic female was hospitalized for the evaluation of a coin lesion in the left lung radiography. She has no history of previous neoplasm or symptom referable to the central nervous system. Chest computed tomography (CT) demonstrated a 9 x 14 mm, round, noncalcified, well-demarcated lesion in the left upper lobe of the lung (S(1+2)). For diagnostic purposes, enucleation of the tumor was performed. The resected specimen revealed histologically classical typical meningioma. Because postoperative magnetic resonance imaging (MRI) of the brain did not show any intracranial mass, this case was and diagnosed as a primary pulmonary meningioma. The patient was discharged with no complication, and alive without recurrence of disease 14 months after surgery.
Female↗
Effects of grape seed proanthocyanidins extract on mandibles in developing rats.
OBJECTIVE: Grape seed proanthocyanidins extract (GSPE), a flavonoid, has a beneficial effect on physical health, which may include the health of bone. The purpose of the present study was to investigate the effects of GSPE on mandibular bone by examining trabecular and cortical bone density, mineral content, and non-invasive bone strength in low-calcium diet rats. MATERIALS AND METHODS: Wistar male rats at 5 weeks old (n = 40) were divided into control (A), low-calcium diet (B), low-calcium diet plus standard diet (C), and low-calcium diet plus standard diet with supplementary GSPE (D) groups. Following 3 weeks of a calcium-restricted diet, group D rats were given 3 mg of GSPE as supplement in 100 g of a standard diet for the next 3 weeks. Following the 6-week experimental period, mandibular bones were examined using peripheral quantitative computed tomography (pQCT). RESULTS: There were no significant differences in body weight or trabecular bone area among the four groups. Trabecular bone density, and trabecular bone mineral content, cortical bone density, cortical bone cross-sectional area, and cortical bone mineral content were significantly higher in group D than in C. Further, Stress-strain index (SSI) values of xSSI and ySSI in group D were significantly higher than in C, although there was no significant difference in pSSI value between those two groups. CONCLUSION: Our results suggest that GSPE treatment caused an increase in both bone formation and bone strength in rat mandibles.
Animals↗
Gene expression profiling of mouse condylar cartilage during mastication by means of laser microdissection and cDNA array.
Little is known about the mechanisms of mandibular condylar growth. In this study, gene expression in the mandibular condylar cartilage of young post-natal mice was monitored by means of a cDNA microarray, real-time PCR, and laser microdissection before and after the initiation of mastication (newborn, 7 days, 21 days, initiation of mastication, and 35 days). Insulin-like growth factor-1 (IGF-I), transforming-growth-factor-beta-2 (TGFbeta2), and aggrecan mRNAs were clearly expressed at 21 days, while the expression of osteopontin mRNAs was most clear at 35 days. Parathyroid-hormone-related protein (PTHrP), Indian-hedgehog (Ihh), and insulin-like growth factor-2 (IGF-2) mRNAs were clearly expressed during lactation (newborn and 7 days). Heat-shock-protein 84 (HSP-84) and heat-shock-protein 86 (HSP-86) were clearly expressed at 35 days. These results revealed that gene expression changed during mandibular condylar cartilage growth, and that, interestingly, these changes coincided with the initiation of mastication.
Aggrecans↗
Characteristics of a newly developed dentomaxillofacial X-ray cone beam CT scanner (CB MercuRay): system configuration and physical properties.
OBJECTIVE: The purpose of this paper is to present the system configuration and physical properties of a new dentomaxillofacial X-ray cone beam CT system (CB MercuRay) being developed. METHODS: The system consists of an image intensifier and a cone beam X-ray source. There are two different models of this system, each with a different size image intensifier, 9" or 12". Each system has three field of view (FOV) modes. The 12" system has facial (F), panoramic (P) and implant (I) FOV modes. The 9" system has P, I and dental (D) modes. Images produced by these systems consist of 512 x 512 x 512 isotropic voxels. Physical properties such as resolution, noise and distortion of the images were evaluated in this study. Modulation transfer function (MTF) was measured using Boone's method. Image noise was measured as the standard deviation of the CT value in water. Circularity of the axial images yielded by the two models was measured using an 8 mm diameter acrylic pipe phantom. RESULTS: The resolving power at a MTF of 0.1 in the D mode was over 2.0 lp mm(-1), suggesting that this system yields images of high resolution. The standard deviation of the CT value in water was approximately 80, which is thought to be greater than that of conventional CT. The circularity of images of the pipe phantom was 99% of the ideal value. CONCLUSION: This study shows that our newly developed cone beam CT system produces high resolution three-dimensional volumetric images that will be useful for the examination of dentomaxillofacial disorders.
Adult↗
Effects of grape seed proanthocyanidins extract on rat mandibular condyle.
We investigated the effects of grape seed proanthocyanidins extract (GSPE) on bone formation by examining total and cortical bone mass, density, architecture, and strength non-invasively using mandibular condyles of Ca-restricted rats. Forty Wistar male rats, each 5 weeks old, were divided into control (C), low-Ca diet (LCaD), low-Ca diet-standard diet (LcaD x SD), and low-Ca diet x Estandard diet with supplementary GSPE (LcaD x SD+GSPE) groups. In LCaD x SD group, after the bone debility was induced by low-Ca diet, a standard diet therapy was given. In LCaD x SD+GSPE group, after the bone debility was induced by low-Ca diet, a standard diet therapy with supplementary GSPE was given. Each mandibular condyle was examined using peripheral quantitative computed tomography (pQCT). There were no significant inter-group differences in body weight seen throughout the experimental period. In LcaD x SD+GSPE, cortical bone cross-sectional area and mineral content were not significantly different from C, while bone mineral content was significantly higher in LcaD x SD+GSPE than in LcaD x SD. Cortical bone density of LcaD x SD+GSPE was not significantly different from that of C, however, that value in LCaD and LcaD x SD was significantly lower than that. The cross-sectional (bending) moment of inertia values in LcaD x SD+GSPE were the highest among all groups, though they did not differ significantly from those in C. Further, the cross-sectional (bending) Stress/Strain Index (SSI) values in LcaD x SD+GSPE were statistically similar to those in C, however, not significant higher than in LcaD x SD. These results suggest that GSPE treatment would increase both bone mass and bone strength on the rat mandibular condyles.
Animal Feed↗
Multiple superconducting phases in new heavy fermion superconductor PrOs4Sb12.
The superconducting gap structure of recently discovered heavy fermion superconductor PrOs4Sb12 was investigated by using thermal transport measurements in magnetic field rotated relative to the crystal axes. We demonstrate that a novel change in the symmetry of the superconducting gap function occurs deep inside the superconducting state, giving a clear indication of the presence of two distinct superconducting phases with twofold and fourfold symmetries. We infer that the gap functions in both phases have a point node singularity, in contrast to the familiar line node singularity observed in almost all unconventional superconductors.
Journal Article↗
The role of craniofacial growth in leptin deficient (ob/ob) mice.
OBJECTIVES: To elucidate the role of leptin on maxillo-facial morphological growth using hereditary obesity model ob/ob mice, and to examine the presence of the leptin receptor gene expression in the mouse condylar head cartilage. DESIGN: Leptin was intraperitoneally administered once a day in 10 C57BL/6J (lean) and 10 C57BL/6J-ob (ob/ob) mice (leptin administration group), and phosphate-buffered saline (PBS) in 10 lean and 10 ob/ob mice (PBS administration group), between the fifth and 11th week after birth. The amount of fat, the body amount without fat, the rate of body fat, and the width of the condylar cervical area were measured during the 11th week, and roentgenographic cephalometric analysis was performed at the fifth, eighth, and 11th week. Furthermore, the condylar head cartilage in C57BL/6J mice was stereoscopically excised to extract total RNA, and RT-PCR method was performed regarding the leptin receptor gene. RESULTS: The body fat amount in ob/ob mice with leptin production insufficiency was greater than that in lean mice, and significant differences were noted in every measurement item regarding maxillo-facial morphology. Recovery of bone length was noted in ob/ob mice by administering leptin. Furthermore, the expression of the leptin receptor gene in the condylar head cartilage was confirmed. CONCLUSION: Exogenous leptin administration leads to significant increases in craniofacial dimensions; and leptin receptors are expressed in mandibular condylar cartilage. These results indicate an important role for leptin in craniofacial growth and morphology. We speculate that leptin's direct peripheral effect on bone and cartilage is closely involved in this role.
Adipose Tissue↗
Modeling of structure, quality, and function in the orthodontic patient.
The advantages of three-dimensional (3-D) imaging technology and solid modeling make it possible to visualize the morphological information. However, lacking in this 'digital patient' is the motion and mechanical properties observed in the living patient. Functional diagnostic techniques such as electromyography and motion analysis could complement the morphological characteristics to be applied in orthodontics. In this review, new computer-assisted analyzing methods are introduced which include visualization of: 1) the 3-D structure and bone density distribution; 2) masticatory-generated forces by using automated finite element modeling (FEM); and 3) the 3-D jaw movement and its motion analysis. In each study, the data from X-ray computed tomography scanning, electromyograms, biting pressure, and digital jaw movement analysis (six axes) are used for calculation. By using these applications, growing changes in bone mineral density distribution of the mandibular cortical bone have been clarified, automated finite element modeling has indicated stress distribution in the craniofacial skeleton, and patient-specific 3-D images of the mandible have been depicted as a motion picture. These studies were completed in 124 living subjects (75 females, 49 males) between 8 and 33 years of age. From these results, malfunctions during mastication were evaluated clearly with the individual patient craniofacial structures and its characteristics. These computer-based visualization techniques can be used to derive much clinically useful information, and to improve the combined evaluation of both static characteristics and dynamic function.
Adolescent↗
Computer-assisted simulations in orthodontic diagnosis and the application of a new cone beam X-ray computed tomography.
Computational simulations which include three-dimensional (3-D) image processing and biomechanical calculations should provide useful information to our research and orthodontic clinic as a clinical tool defined as 'thinking'. In this review, 1) biomechanical simulations applied to predict the mandibular growth; 2) mathematical models of virtual bone cells and 3) 3-D images and solid model simulations for surgical planning are introduced. In biomechanical simulation, biting force, electromyographic (EMG) activity and cephalograms of 32 subjects were applied. Computational results of mathematical model were compared with actual bone growth in a rat. Three-dimensional image and solid model of 14 patients were utilized for their treatment planning. From the results, several concepts of our simulations were confirmed: 1) reaction forces generated by masticatory muscles at the condyle control the direction of mandibular growth; 2) some mathematical models have the possibility to describe the process of bone growth; 3) 3-D image processing software and solid models are necessary for diagnosis and planning of orthognathic surgery. We also believe that the orthodontists can more accurately predict the affects of surgical procedures and orthodontic tooth movement using the new cone beam X-ray computed tomography (CT) (CB MercuRay; Hitachi Medico Technology, Tokyo, Japan) and its advanced application software.
Animals↗