PubMed Health⌕ Search

Biomedical subjects

K Maki

Publications and source records attributed to K Maki.

12 recordsLinked to original sources
PubMed1992

[Adequate Ca level for dialysis patient (study on medical therapy for secondary hyperparathyroidism in dialysis patient)].

We evaluated our medical therapy of secondary hyperparathyroidism (2 degrees HPT) in dialysis patients. Ten hemodialysis patients with 2 degrees HPT (PTH-C; higher than 10ng/ml) were treated with vitamin D3, large amounts of phosphate binders and calcitonin and data over three years were examined. Our data showed that 2 degrees HPT is manageable by medical therapy. Furthermore, we suggest that the adequate Ca level for dialysis patients is to maintain the Ca (mEq/1) xP (mg/dl) at less than = 35. When plasma P is less than 6mg/dl. The maintenance of this Ca level successfully treated 2 degrees HPT, and this prevent the appearance of renal osteodystrophy (ROD) in dialysis patients.

Adult↗
PubMed1991

A cationic detergent, cetyltrimethylammonium bromide (CTAB), selectively dissociates the intermediate filament of the fibroblast.

We investigated conditions for selective solubilization of the intermediate filament (IF) of BHK-21 cells, and found that a cationic detergent, cetyltrimethylammonium bromide (CTAB), was effective for rapid dissociation of IF into the monomeric form. More selective dissociation was performed with a combination of CTAB and Tween 40. The CTAB-dissociated vimentins were unstable, but the breakdown of them was successfully blocked by leupeptin. Thus, with our extraction buffer, composed of 1% CTAB, 1% Tween 40, 10 mM Tris-HCl (pH 7.4) and 25 micrograms/ml leupeptin, almost all of the vimentin as well as the desmin were solubilized, while two thirds or more of actins were retained in the CTAB/Tween-insoluble fraction.

Animals↗
PubMed1990

[An experimental study of the effect of food on osteoporosis especially on the mandible].

Three-week-old male rats of the Wistar strain given standard formed food (MF formed food) were grouped as controls. Those given calcium-deficient food for three weeks and then standard formed food after experimental osteoporosis induced in the rats were grouped as the experimental group. Experimental periods were for 4 and 6 weeks and the effect of the food on osteoporosis was investigated. The results were as follows: 1. Bone density of the alveolar bone showed significantly lower values for the experimental group than for the controls. 2. In comparison of relative Ca ratios (Ca/[Ca]c) and of P ratios (P/[P]c) on an X-ray microanalyzer, the controls showed higher values each week than the experimental group. 3. Compressive strength of the mandible in the experimental group decreased compared with the controls.

Animals↗
PubMed1977

[Human proinsulin. C-peptide radioimmunoassay method. 125I labeling of human proinsulin. C-petide].

125I-labelled human-C-peptide was prepared by chloramin T method, enzymic method and active ester method, respectively. Using respective 125I-labelled human-C-peptides in human proinsulin-C-peptide RIA, we compared the binding (Bo/T%) to antibody, displacement by standard human-C-peptide, the recovery test and stability. The usable 125I-labelled antigen for human proinsulin-C-peptide RIA could be prepared by chloramin T method and enzymic method wich labelled 125I to tyrosyl human proinsulin connecting peptide, and active ester method which conjugates 125I-labelled active ester to human proinsulin connecting peptide. The differences among those 125I-labelled antigens was not observed in displacement (B/Bo%) by standard human-C-peptide and the recovery test. In the case of constant preparation of 125I-labelled antigen for RIA, the enzymic method was the best from the viewpoint the reaction ratio is stable and stability of Bo/T% is good.

C-Peptide↗
PubMed1977

[Studies on human proinsulin C-peptide radioimmunoassay method--preparation of antiserum and its specificity-- (author's transl)].

The antisera using at final dilution of 1 : 10,000 have been prepared by immunizing synthetic human proinsulin connecting peptide to rabbits for human proinsulin C-peptide radioimmunoassay. The cross reactivities of human proinsulin C-peptide derivatives with the prepared antisera were reduced by leaving amino acid residues from N terminal, although this phenomenon was a little different among antisera. Those results suggested that main antigen determinant in N terminal 31-38 of human proinsulin connecting peptide. The cross reactivities of other animal proinsulin C-peptide and other peptide hormones with the prepared antissera were not recognized at 10(3) p mole/ml.

C-Peptide↗
PubMed1977

[Human proinsulin-C-peptide radioimmunoassay method; stability of the assay kit].

We have been studies time sequent stability each on standard human-C-peptide, human-C-peptide antiserum, 125I-tyrosyl human-C-peptide and the assay kit (all reagent) which is necessary in human proinsulin-C-peptide radioimmunoassay(RIA). Also we measured using this assay system, human proinsulin C-peptide in blood after oral administration of glucose to normal subject. Standard human-C-peptide and human-C-peptide antiserum were very stable on storage at 4 degrees C, 125I-tyrosyl human-C-peptide was unstable as compared with the former two, The stability of the assay kit was influenced by the stability of 125I-tyrosyl human-C-peptide, and was stable at 4 degrees C for ten weeks after preparation. Three lots of the assay kit prepared at different period showed almost same stability. We think this assay system using the assay kit is satisfactory in respect of stability. The measured values of human proinsulin-C-peptide in blood, using this assay system, showed insulin secretory reaction after oral administration of glucose.

C-Peptide↗