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Biomedical subjects

K Marcinka

Publications and source records attributed to K Marcinka.

16 recordsLinked to original sources
PubMed1989

Effects of pH and ionic strength on precipitation of phytopathogenic viruses by polyethylene glycol.

The effects of ionic strength of the solution (changed by varying NaCl concentrations or buffer molarity) on the precipitation with polyethylene glycol (PEG) 6000 were studied on phytopathogenic viruses of different morphology: the isometric red clover mottle virus (RCMV), rod-shaped tobacco mosaic virus, flexuous potato virus X (PVX) and bacilliform alfalfa mosaic virus. With increasing NaCl concentration or buffer molarity up to a certain level (0.1 mol/l), the efficiency of PEG precipitation increased. This relationship did not apply to PVX. The effects of pH on PEG precipitation were studied on RCMV. The efficiency of precipitation increased with decreasing difference between pH of the solution and pI of the virus.

Buffers↗
PubMed1986

Studies on preparation of a tick-borne encephalitis (TBE) vaccine from the Skalica strain.

Diethylether-treated vaccine against tick-borne encephalitis (TBE) represents a new type vaccine consisting of lipid-free and antigenically efficient components instead of whole virus particles. The TBE virus strain designated Skalica was used for vaccine preparation. This strain is thermosensitive, produces small plaques under agar overlay, is nonpathogenic for adult white mice following subcutaneous (s.c.) application and causes threshold viraemia in host animals. The vaccine was harmless and immunogenic as evidenced by experiments on white mice. Antibodies to TBE virus strain Ir 13 present in human healthy population of a natural TBE focus showed similar levels when tested with the Skalica strain. The Skalica virus strain can be recommended for preparation of the vaccine against TBE.

Animals↗
PubMed1979

The effects of polyacrylic acid and virazole on the replication and component formation of red clover mottle virus.

Polyacrylic acid and Virazole inhibited the replication of red clover mottle virus (RCMV) in systemically infected pea (Pisum sativum) plants, as shown both by serological and infectivity tests. The Virazole-induced changes had little effect on the proportion of the three components in the total virus yield, but treatment with polyacrylic acid markedly increased the proportion of the RNA-free top component. This suggests that either viral RNA synthesis or RNA encapsidation was inhibited.

Acrylates↗
PubMed1977

Disintegration of red clover mottle virus virions under different conditions of storage in vitro.

Red clover mottle virus was kept in 0.1 M phosphate buffer, pH 7.0, at 4, --20 and --60 degrees C for 1 and 4 weeks and 3 months. The degree of virion disintegration was the highest at --20 degrees C and the lowest at 4 degrees C. Two cycles of freezing and thawing destroyed the virions. In frozen samples (at both --20 and --60 degrees C), the nucleoprotein sedimentation component M was disintegrated more than component B.

Freezing↗
PubMed1975

Some properties of clover blotch virus.

Clover blotch virus (CBV) was purified by differential centrifugation or precipitation with polyethylene glycol 6000 or 20000. Analytical centrifugation of purified CBV preparations revealed 2 components, the proportions of the lighter (96 S) nad heavier (112 S) being 88 and 12%, respectively. RNA isolated from purified CBV represented a micture of several RNA species of different molecular weight and its infectivity was by about 2 log units lower than that of the original nucleoprotein. CBV virions are isometric with a diameter of 28 nm. CBV is tentatively included into the Cucumovirus group.

Plant Diseases↗