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K Mase

Publications and source records attributed to K Mase.

At least 19 recordsLinked to original sources
PubMed2000

Application of the p53 and K-ras gene mutation patterns for cytologic diagnosis of recurrent lung carcinomas.

BACKGROUND: Cytologic specimens are one of the most important materials for lung carcinoma diagnosis, because they can be used in mass screening for lung carcinoma and early detection of cancer recurrence by examination of sputum and pleural fluid. METHODS: To prove the potentiality of the cytologic specimens to be subjected to molecular detection of recurrent lung carcinomas, the authors enrolled 16 patients who had undergone surgical treatment for lung carcinoma with recurrence detected by malignant pleural fluid. First, they examined K-ras gene and p53 tumor suppressor gene abnormalities in resected tumors by polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP) analysis. Next, using a microdissection method, they investigated the use of cytologic specimens such as pleural fluid for the detection of recurrence by finding the same mutations observed in the initially resected tumor. RESULTS: Seven abnormally shifted bands were detected among six patients by PCR-SSCP analysis of surgical materials. Five of 7 abnormally shifted bands (71.4%) also were detected from microdissected malignant cells in cytologic smears. In two cases, they detected mutations by using single malignant cells in pleural fluid. CONCLUSIONS: The authors successfully detected the same mutations in recurrent cytologic specimens as those in the initially resected tumors by PCR-SSCP analysis. These findings suggest that the p53 and K-ras gene mutation patterns are effective markers for the detection of recurrent lung carcinoma in cytologic specimens. Cancer (Cancer Cytopathol)

Aged↗
PubMed2000

Amplotyping of microdissected, methanol-fixed lung carcinoma by arbitrarily primed polymerase chain reaction.

The arbitrarily primed polymerase chain reaction (AP-PCR) was used to detect somatic genetic alterations in lung carcinomas. DNA fingerprints generated by a single arbitrary primer were compared between normal and tumor tissues of the same individuals. We adapted the technique to the use of tissue fixed with methanol, which allowed the analysis of small areas of tissue by microdissection. This improvement of the fingerprinting technique permitted the study of tumors at early stages of progression. Loss of sequences from chromosome 7 was detected in 41.7% of adenocarcinomas and from chromosome 22 in 84.6% of small-cell carcinomas. Gains of sequences from chromosomes 1, 8 and 13 were detected in more than 40% of adenocarcinomas and in chromosome 2 in 63.3% of squamous-cell carcinomas. Our results indicate that allelic imbalances at these chromosomal regions are common genetic abnormalities in lung carcinomas. Loss of sequences from chromosome 22q13.3, found in 11 of 13 small-cell carcinomas, were confirmed by microsatellite PCR analysis. We show that the use of our improved AP-PCR fingerprinting permits the detection of both losses and gains of novel chromosomal regions early during lung cancer development. Our results indicate that early-stage tumors tend to have more allelic imbalances than relatively advanced tumors, suggesting a high tumor genetic heterogeneity in the early stages of lung tumor progression.

Adenocarcinoma↗
PubMed2000

Occurrence of ommochrome-containing pigment granules in the central nervous system of the silkworm, Bombyx mori.

Dark-red pigment granules were found in the brain and ganglion of the normal strain of the silkworm, Bombyx mori, by light microscopy. No other pigmentation was seen in the brain or ganglia. Electron microscopy showed that the granules were electron-dense. The granules were similar to the ommochrome-containing pigment granules that are present in the epidermal cells of the quail mutant, as previously reported. The pigment in the larval central nervous system (CNS) of the normal silkworm was identical to the ommin standard with respect to the absorption spectrum, the infrared spectrum, and the Rf value in thin-layer chromatography (TLC). After acid hydrolysis of the pigment, 3-hydroxykynurenine was detected by TLC. The pigment granules in the CNS contained mainly ommin. An ommochrome-binding protein was also detected in the CNS by in vitro binding studies and Western blotting. The ommochrome granules may have an important function in the CNS of the silkworm.

Animals↗
PubMed2000

Firm adherence of Staphylococcus aureus and Staphylococcus epidermidis to human hair and effect of detergent treatment.

Staphylococcus aureus and S. epidermidis are common pathogens in hospitals, and care should be taken not to disseminate these organisms among patients. We have focused on human hair as a source of bacterial contamination. We treated hair with culture solutions of S. aureus and S. epidermidis, and then performed scanning electron microscopy. Bacteria were detected on the surface of the cuticles of the hair, and the attached bacteria were not completely removed even by repeated washing with detergents. These results suggested that hair could be a source of bacterial contamination and indicated the importance of decontamination of hair.

Bacterial Adhesion↗
PubMed1999

DNA methylation and expression of p16(INK4A) gene in pulmonary adenocarcinoma and anthracosis in background lung.

The p16 (CDKN2/MTS-1/INK4A) tumor-suppressor gene is frequently inactivated by DNA methylation in lung carcinomas. To clarify whether background anthracosis may play a role in DNA methylation and inactivation of the p16 gene, we examined DNA methylation of the p16-promoter region by methylation-specific polymerase chain reaction, and p16 expression immunohistochemically, and compared the results with the level of background anthracosis which was measured by an original quantitative method. At autopsy, DNA methylation of the p16 gene was observed in 6/19 tumors (32%) from patients who had died of pulmonary adenocarcinoma. The degree of background anthracosis (the effect of extrinsic carcinogenic factors) (mean absorbance value, A = 0.715) of the cases with p16-gene methylation was significantly higher than that without methylation (mean A value = 0.298). p16 expression was inactivated in all tumors with p16-gene methylation. The mean A value of black dust matter deposition in cases with normal expression of p16 (A = 0.151) was significantly lower than cases with abnormal expression of p16 (A = 0.531). These results indicate that the level of background anthracosis is closely associated with inactivation of p16 expression and also DNA methylation of the p16-gene promoter region in pulmonary adenocarcinogenesis. Int. J. Cancer (Pred. Oncol.) 84:609-613, 1999.

Adenocarcinoma↗
PubMed1998

The implication of anthracosis in the development of pulmonary adenocarcinoma.

The relationship between anthracosis, which is the deposition of black dust matter in the lung parenchyma, and the development of pulmonary adenocarcinoma has not been fully characterized. In order to clarify whether background black dust matter deposition could be implicated in the development of pulmonary adenocarcinoma, we measured the level of anthracosis at autopsy in 47 patients who had died of pulmonary adenocarcinoma. Both lungs of all 47 cadavers were examined. Twenty-micrometer sections were cut from formalin-fixed, paraffin-embedded blocks of the largest cut surface of each lung. Black dust matter was extracted from the sections and blotted onto a nitrocellulose membrane. The density of the blotted black dust matter was then analyzed using an imaging densitometer. There were no significant differences in the density of black dust matter deposition between lungs affected by pulmonary adenocarcinoma and control lungs. However, well differentiated adenocarcinomas tended to develop more frequently than poorly differentiated ones in lungs showing less deposition. We found a very strong correlation between the degree of black dust matter deposition and smoking history. Patients with severe anthracosis tended to have a poorer prognosis than those with mild anthracosis.

Adenocarcinoma↗
PubMed1998

[Purpura simplex].

Explore the source record for details and available documents.

Diagnosis, Differential↗
PubMed1996

[Anesthetic management of a pediatric patient with Cornelia de Lange syndrome].

We did anesthetic management on a pediatric patient with Cornelia de Lange syndrome presenting a peculiar look, variable physical deformities, and some growth and mental retardations. An 8-year-old male patient was scheduled for inguinal hernioplasty under general anesthesia. This patient had been suffering from dehydration for apodeisis and self-destructive tendency due to psychologic stress. Anesthesia was induced and maintained with sevoflurane and N2O in oxygen. The area of skin incision was infiltrated with 0.25 % bupivacaine for the purpose of post-operative analgesia, and as a result, psychologically and physically stable condition was obtained during post-operative periods. It is necessary to understand not only the patient's physical conditions but also mental ones in order to make the anesthetic management safer and more satisfactory.

Analgesia↗
PubMed1995

[Criteria of limited operation for bronchial carcinoid].

The purpose of this study is to establish criteria of limited operation for bronchial carcinoid. Ten cases surgically treated in our hospital and 47 cases reported in Japan from 1981 to 1992 were examined. Thirty-nine cases had typical carcinoid tumor and 18 had atypical. Limited operation, such as pulmonary segmentectomy, wedge resection or partial resection, was performed in 12 cases. In these, all patients of typical type were alive except for a case died of other disease. However, two patients of atypical type died of distant metastasis of the tumor. No lymph node metastasis was revealed in all cases of typical type. On the contrary, in cases of atypical type, six had n1 and two had n2 disease. Moreover, two cases with n1 disease had pulmonary metastasis. Therefore, patients with typical bronchial carcinoid can be cured by limited operation, but radical operation should be indicated for atypical bronchial carcinoid.

Adult↗
PubMed1994

[New endoscopic laser treatment for unilateral vocal cord paralysis after pulmonary resection].

In two patients who had developed aspiration due to left recurrent laryngeal nerve injury during radical resection for lung cancer, low power Nd:YAG laser irradiation of the paralyzed vocal cord was performed through a bronchofiberscope. With irradiation, the paralyzed vocal cords of both patients swelled and became fixed at the median portion of the glottis. Thereafter, aspiration disappeared. Thus, this new application of Nd:YAG laser irradiation is apparently quite useful in treating aspiration due to unilateral vocal cord paralysis immediately after pulmonary resection.

Bronchoscopy↗