PubMed Health⌕ Search

Biomedical subjects

K McColl

Publications and source records attributed to K McColl.

26 records · Page 2Linked to original sources
PubMed1989

Examination of mucosal surfaces by scanning electron microscopy before and after removal of debris.

A rapid method is described whereby gastrointestinal biopsy specimen surfaces can be examined by scanning electron microscopy with overlying tract contents (debris) intact, and also re-examined after cleaning to determine the structure of the underlying mucosal surface. The conductive coating of gold is removed using mercury, a non-wetting agent. The specimen surface is suitably cleaned of debris after a brief ultrasonication in absolute ethanol which mixes with the transitional fluid (CO2) used for critical point drying.

Animals↗
PubMed1989

Stability of murine monoclonal anti-A, anti-B and anti-A,B ABO grouping reagents and a multi-centre evaluation of their performance in routine use.

We have previously reported the production of 3 murine monoclonal reagents for ABO typing (designated ES-9, ES-4 and ES-15). This study presents results of tests of stability of these 3 reagents, together with a fourth murine monoclonal antibody (LM103/107). In addition, data are also presented from a multi-centre evaluation of the performance of the murine monoclonal reagents in routine ABO typing of both donors and patients using a wide variety of techniques, both manual and automated. The potency and stability of the 4 monoclonal antibody based reagents is compared with a broad selection of monoclonal and polyclonal ABO typing reagents. The reagents used for comparison were produced by European and United States manufacturers in both the public and private sector and are widely used in routine ABO typing. The Scottish monoclonal reagents have been used successfully to ABO type over 500,000 blood samples in 7 centres within the UK, with no discrepant results.

ABO Blood-Group System↗
PubMed1989

Polyethylene glycol in the production of blood grouping reagents: some further advantages.

The use of various water-soluble non-ionic polymers, including polyethylene glycol (PEG) 8000, has been described in the preparation of diagnostic blood grouping reagents. The major advantages of using PEG in this way are ease of preparation and the ability to concentrate antibodies. We describe here two previously unreported advantages of using PEG: (i) substantial reductions in the volume of red cells required for adsorption of isoagglutinins; and (ii) the ability to store PEG precipitates in a 'dry' form substantially reduces storage requirements.

Blood Grouping and Crossmatching↗
PubMed1988

Fatal respiratory disease in Nilgiri tahr: possibly malignant catarrhal fever.

Nilgiri tahr (Hemitragus hylocrius) are native to India and are a rare zoo exhibit. This report describes an acute respiratory disease in tahr that caused the death of 15 of 16 animals in an extensive exhibit of about 35 acres where they were housed together with a variety of other exotic species of ruminants. The deaths occurred in two separate outbreaks and were associated with losses from malignant catarrhal fever in other ruminants in the exhibit. The most prominent clinical sign was severe dyspnea, and death occurred within five days. The principal lesions were an acute nonsuppurative inflammation of the respiratory tract and pulmonary vessels, lymphadenopathy and lymphoid cell infiltration in the organs of some animals. It was conjectured that the tahr died of a unique pneumonic form of malignant catarrhal fever. Attempts at viral isolation were negative.

Animals↗
PubMed1980

Mortality in little penguins (Eudyptula minor) along the coast of Victoria, Australia.

Forty-eight little penguins (Eudyptula minor) consisting of 21 (43.7%) mature, 18 (37.5%) juvenile and nine (18.7%) of undetermined age, from 10 Victorian coastal localities were examined during 1977-78. Thirty-seven (77%) of all penguins were in poor body condition with moderate to heavy burdens of internal and external parasites. Acute parasitic gastric ulceration with accompanying hemorrhage, was implicated in the death of four birds. Chronic gastric ulcers were thought to have caused appetite depression and starvation in 28 birds. Other significant lesions encountered included renal coccidiosis, parasitic cholangiohepatitis and pulmonary aspergillosis. It is suggested that the increased mortality experienced during 1977-78 was due to starvation or to exacerbation of the effects of existing parasite burdens on starving and exhausted birds.

Animals↗
PubMedDate not supplied

Comparative bioavailability of two flurbiprofen products: stereospecific versus conventional approach.

In this randomized, crossover study comparing the bioavailability of a film-coated (Ansaid) with a sugar-coated (Froben) 100 mg tablets of racemic flurbiprofen in 23 healthy young men, no significant differences were found for Cmax, tmax or AUC, using a nonstereoisomeric assay for flurbiprofen. Minor differences in the appearance of flurbiprofen in serum during the first 30-min post-dosing period were noted, with Ansaid appearing earlier than Froben. These differences likely reflect dissolution rate dissimilarity between the two products. Stereospecific determinations demonstrate a small (7.8 per cent) but significant difference in AUC of the active S-configuration (Froben greater than Ansaid). No significant differences between Ansaid and Froben were found for tmax or Cmax for the S-flurbiprofen. In bioequivalency studies of chiral drugs, stereospecific approaches provide a more accurate assessment of products.

Adolescent↗