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Biomedical subjects

K McLaughlin

Publications and source records attributed to K McLaughlin.

10 recordsLinked to original sources
PubMed1992

Binding of human single-stranded DNA binding protein to DNA damaged by the anticancer drug cis-diamminedichloroplatinum (II).

The chemotherapeutic drug cis-diamminedichloroplatinum (II) covalently binds to DNA resulting in a variety of adducts and cross-links which are thought to be responsible for the toxicity of the drug. We have used the gel mobility shift assay to detect proteins which bind to DNA treated in vitro with cis-diamminedichloroplatinum (II) and have identified two complexes which bind with increased affinity to cis-diamminedichloroplatinum (II)-damaged DNA. Using monoclonal antibodies we have shown that one complex, B1, contains human single-stranded DNA binding protein, a protein known to be involved in the in vitro repair synthesis assay of mammalian excision repair.

Antibodies, Monoclonal↗
PubMed1992

Nursing theory as a base for a computerized nursing information system.

This article illustrates how nursing theory can be used in a computerized nursing information system (CNIS). Before development of such a system can begin, data relationships must be specified so that: (a) the relationships are specific enough to support a computerized relational base and (b) the resultant information can support nursing science. Both goals were achieved through three interrelated processes: (a) validation of a practice model based on Orem's self-care deficit nursing theory, (b) classification of clinical data into theory-related categories, and (c) establishment of data relationships to yield meaningful information.

Databases, Factual↗
PubMed1992

Blood pressure lowering and cerebral blood flow: a comparison of the effects of carvedilol and propranolol on the cerebral circulation in hypertensive patients.

The first part of this article concerns the general relation between blood pressure lowering and cerebral blood flow. Factors known to affect cerebral blood flow are reviewed, and recent advances in the field of innervation of cerebral blood vessels are outlined. Special emphasis is placed on the importance of the phenomenon of autoregulation and how this is disturbed in patients with hypertension. Not all antihypertensive drugs exert the same action on the cerebral circulation to produce the same blood pressure-lowering effect. In addition, the actions exerted by common antihypertensive drugs on the cerebral circulation are described. The second part of the article provides a comparison between the effect of carvedilol and that of propranolol on blood pressure reduction and cerebral blood flow. In a double-blind, crossover study involving 14 patients with mild-to-moderate hypertension, carvedilol was shown to have no effect on cerebral blood flow, whereas there was a slight tendency for lower flows to occur with the use of propranolol. Because of the small sample size, statistical significance was not reached. These results are consistent with other recently published findings.

Adolescent↗
PubMed1991

Single step selection of cis-diamminedichloroplatinum(II) resistant mutants from a human ovarian carcinoma cell line.

We have shown that cis diamminedichloroplatinum-(II) (DDP) resistant mutants can be isolated from the human ovarian carcinoma cell line A2780 using a single-step selection protocol with DDP. DDP resistant colonies were calculated to be present at a frequency of 1.7 x 10(-6)/viable cell using a fluctuation analysis. The mutational origin of these surviving colonies is inferred by the fact that their frequency is increased by treatment of the A2780 cells with the chemical mutagen ethyl methane-sulfonate, with a maximum frequency observed after a 3-day expression time. Independently isolated clones maintain, in the absence of selection, a DDP resistant phenotype up to 7-fold more resistant than the parental A2780 cells. The resistance modifiers aphidicolin and buthionine sulfoximine have no effect on the frequency of DDP resistant mutants. Therefore neither of these drugs appears to have an effect on increasing the sensitivity of DDP resistant mutants existing in a cell population prior to DDP exposure.

Aphidicolin↗
PubMed1991

Orem's general theory of nursing and community nursing.

The purpose of this article is to examine the way in which theoretical concepts of community articulate with Orem's theory. Philosophical and theoretical perspectives of community are presented as the basis for developing a model for community nursing. The development of nursing theory and science related to the concept of community proceeds from an understanding of community and its articulations with nursing. Three basic models of community are identified and described: (a) atomistic/contractarian, (b) organic/functional, and (c) mutual/personal. Orem's theory can be legitimately viewed as having meaning for nursing when the community is viewed from any of the three models. It is not limited to the use of "aggregate" models. The distinction comes in the view of community consistent with the underlying philosophical view of the nature of person expressed or inherent in the nursing theory. A general model for the community as a unit of service, congruent with Orem's theory, is presented.

Community Health Nursing↗
PubMed1990

Determination of selenium in blood plasma and serum by flow injection hydride generation atomic absorption spectrometry.

A flow injection hydride generation atomic absorption spectrometric (AAS) method has been used to determine the selenium concentrations of human serum and plasma samples following digestion with nitric, sulphuric and perchloric acids. In the hydride generation process, reduction was carried out by sodium tetrahydroborate to produce a hydride that was atomized in a flame-heated atomisation cell. The method had a detection limit of 1.2 ng ml-1 and a sensitivity of 2.1 ng ml-1. Within-run precisions of 5.8% at 20 ng ml-1 and 4.5% at 80 ng ml-1, and between-run precisions of 4.8% at 69 ng ml-1 and 3.4% at 80 ng ml-1 were obtained. An inter-laboratory comparison study with a graphite furnace AAS method was carried out and the results showed excellent agreement. The flow injection method of sample introduction allowed the use of a sample volume of 330 microliters with an injection rate of 90 injections per hour.

Borohydrides↗
PubMed1990

Validating nursing theory for use within a computerized nursing information system.

Computerized nursing information systems (CNISs) exemplify the application of computer science to nursing knowledge. For nursing knowledge to be a basis for CNIS development, variable relationships must be clearly defined, thus allowing transformation of data into knowledge. Nursing theories define variable relationships; these definitions, however, often are not specific enough on which to base data-based nursing information systems. This article describes how a nursing process model was derived from Self-Care Deficit Nursing Theory, the process model validated, and data relationships established within the model prior to its use as a structure for a CNIS.

Computer Simulation↗
PubMedDate not supplied

Shaping the future. The marriage of nursing theory and informatics.

The link between nursing theory and computerized nursing information systems is explored. In particular, the use of Self-Care Deficit Nursing Theory in the development of an information processing model for use in a computerized nursing information system is described. This development is discussed with reference to the American Nurses' Association design criteria for computerized nursing information systems. There is some indication that the design criteria do not adequately address the relationships among nursing theory, nursing processes, and computerized nursing information systems.

American Nurses' Association↗