Biomedical subjects
Publications and source records attributed to K Melby.
6,042 strains of Staphylococcus aureus, Streptococcus faecalis, Proteus mirabilis, Escherichia coli, Klebsiella sp. and Pseudomonas sp. were tested against tobramycin and gentamicin. Among all species studies, except Pseudomonas sp, gentamicin turned out to be slightly superior to tobramycin.
A study on the interaction of different sera and polymorphonuclear cells (PMN) with reference to the ABO blood group system on the phagocytosis of a radiolabelled strain of E. coli is reported. Using untreated sera, O cells were found to be the least sensitive and AB cells the most sensitive to reduction in phagocytic activity. No reduced phagocytic capability relative to the different sera used was observed when heat-inactivated sera were applied. Aspects of these results are discussed.
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A system for the study of phagocytosis by human polymorphonuclear cells (PMN) is presented. The leucocytes are harvested from heparinized whole blood by the Bøyum method and transferred to glass tubes to yield glass adherent monolayers of leucocytes, approximately 80% of which were PMN. A strain of E. coli labelled by 32P served as test organism.
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Using 32P-labelled Escherichia coli, the effect of 8 antibacterial agents on the ability of rat polymorphonuclear cells to ingest the labelled bacteria was studied with and without serum present during the ingestion phase. No impairment of ingestion was observed when testing gentamicin, cephalothin and trimethoprim. High doses of oxytetracycline, doxycycline, colistin, erythromycin and chloramphenicol seemed to impair ingestion. The effect was most pronounced when applying 100 mug/ml of doxycycline: the ingestive capability was reduced to 25% without serum and 24% with serum present compared to untreated controls. 83 mug/ml of colistin reduced theingestion to 39% without serum present, this effect being totally eliminated when serum was present during the ingestion phase.
The effect of 8 antibacterial agents on the elimination of 32P-labelled Escherichia coli from rat polymorphonuclear cells was studied. None of the drugs tested had a detectable influence on the expulsion of labelled products from the cells being exposed to the various drugs during the elimination phase.