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K Merte

Publications and source records attributed to K Merte.

27 records · Page 2Linked to original sources
PubMed1979

[Vital microscopic examination of the periodontal blood circulation of incisors in rats (author's transl)].

This paper describes a method of making microscopic in vivo examinations of the periodontal blood vessels of the lower incisors in rats. On an anesthetized animal, part of the lower jaw is laid open by a U-shaped skin incision and the ligament joining the halves of jaw is cut through. After macrosopic and microscopic preparation, the circulation of blood in the exposed marginal gingiva or in the windowed periodontal space may be observed in vivo by transmitted-light microscopy. The standardized biological model enables the structure and function of the periodontal blood vessels and their normal and disturbed mechanisms of regulation to be examined and compared with conventional histological findings. The functional and morphological parameters are representative of the human periodontium.

Animals↗
PubMed1979

[The systemic circulation and microcirculation of the periodontium vital microscopy and histological studies of incisors of rats (author's transl)].

A new classification of the angioarchitecture and blood flow is proposed in light of the results of vital microscopy studies of the windowed periodontium and gingiva of the incisors of rats. The flow pattern and the rate of blood flow are different in different vessels. The flow of blood in segmented and interconnected arterial and venous vascular sections in the alveolar and marginal periodontium corresponds to what is known as systemic circulation, whereas that in arterioles, capillaries, and venoles must be considered as belonging to what is referred to as microcirculation. Subepithelially, the microcirculatory elements constitute a nutritive, typically ordered capillary network as contrasted with the random spatial arrangement of capillary loops in the alveolar periodontium. Numerous anastomoses provide the basis for possible functional adaptation, leading to the formation of polygonal ring closures in the alveolar region. The interpretations of functional findings are based upon histotopological reexaminations.

Alveolar Process↗
PubMedDate not supplied

Peroxidase reaction as a parameter for discrimination of Streptococcus mutans and Streptococcus sobrinus.

425 strains of mutans streptococci and 12 reference strains were investigated by membrane fatty acid spectra (MFAS) and peroxidase reaction (PR) after aerobic and anaerobic incubation. 423 strains were identified as Streptococcus mutans. The remaining 2 strains were identified as Streptococcus sobrinus. The PR of 29 strains was doubtful; immediately after anaerobic incubation a negative PR changed into a slightly positive PR. To test the diagnostic value of PR the strains were additionally investigated by means of species-specific polymerase chain reactions (PCR). The species-specific PCRs were developed on the basis of the respective genes of 16S rRNA of the pathogens S. mutans and S. sobrinus. Specificity and sensitivity were tested on reference strains (n = 17) and negative control strains (n = 39). The results of this investigation showed that an anaerobic incubation regime could lead to false-positive (S. mutans) or false-negative (S. sobrinus) PR. The 425 MS strains were classified as either S. mutans (n = 420) or S. sobrinus (n = 5). The findings on the reference strains required a reclassification of S. mutans V 100 into S. sobrinus V 100. Summarising, it is possible now to differentiate strains of mutans streptococci by MFAS and PR after aerobic incubation.

Aerobiosis↗