Role of sulfhydryl group in the structure and function of alkaline proteases from a thermophilic actinomycete, Streptomyces rectus var. proteolyticus.
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Binding Sites↗
Biomedical subjects
K Mizusawa
Publications and source records attributed to K Mizusawa.
Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii.
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Chelating Agents↗
Thermophilic Streptomyces alkaline proteinase. II. The role of a sulfhydryl group and the conformational stability.
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Aspergillus↗
Thermophilic Streptomyces alkaline proteinase. I. Isolation, crystallization, and physicochemical properties.
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Amino Acids↗
[An electron microscopic observation on the chiasmal region of the optic nerve in experimental allergic encephalomyelitis].
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Animals↗
[A case of malignant hemangio-endothelioma with metastasis to the cranium, humerus and rib].
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Adult↗
Production of thermostable alkaline proteases by thermophilic Streptomyces.
Conditions for the production of thermostable proteases (alkaline proteinase and carboxypeptidase) by a thermophilic streptomycete (Streptomyces rectus var. proteolyticus) were investigated in 20-liter volumes. Proteinase production was affected by the concentration of defatted soybean powder, its optimum being 1.2% in medium containing 2.0% soluble starch. Relatively high concentration of phosphate (0.3 to 0.4% K(2)HPO(4)) was needed for the maximum enzyme production. A large inoculum size (5 to 10%) was favorable, but the inoculum age did not significantly influence the production. The yield increase of 20 to 30% was obtained by feeding of medium during fermentation. The optimal temperature for proteinase production was 50 C, at which the maximal rate of production was 66.2 proteinase units per ml per hr, whereas at 40 C it was 9.0. Production at 50 C reached the maximum within 12 to 16 hr. The optimal agitation rate was different for the production of proteinase and carboxypeptidase, 400 rev/min for the former and 500 rev/min for the latter. The optimal aeration for proteinase production was 20 to 30 liters/min at 400 rev/min, whereas carboxypeptidase production was not markedly affected by aeration rate. The possibility that carboxypeptidase production was correlated with the shear of mycelium was discussed.
Bacteriological Techniques↗
[Inflammatory tumors in the orbit].
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Adult↗
[Cryotherapy of tumors of the eyelid].
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Adult↗
Long-term postoperative analysis of nutritional status after limited gastrectomy for early gastric cancer.
BACKGROUND/AIMS: An examination was made of the postoperative long-term effects of limited gastrectomy (LG) on nutritional status, as one of the factors that influence quality of life. METHODOLOGY: Nutritional status in 33 patients who underwent LG for early gastric cancer, was compared with that of 36 patients who underwent standard gastrectomy (SG). Nutritional conditions were assessed preoperatively, and then 1 and 2 weeks, 1 and 6 months, and 1, 2 and 3 years after surgery. RESULTS: Postoperative recovery of both body weight and body fat mass was greater in LG than SG, and there were significant differences between the two groups of patients from six months after surgery until three years had elapsed. CONCLUSION: LG minimized the extent of nutritional impairment during long-term recovery from surgery, as compared with SG. LG would seem to be a suitable operative procedure for some patients with mucosal gastric cancer, without reducing radicality for cancer.
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