PubMed Health⌕ Search

Biomedical subjects

K Mogensen

Publications and source records attributed to K Mogensen.

At least 19 recordsLinked to original sources
PubMed1998

Differences in activity between alpha and beta type I interferons explored by mutational analysis.

Type I interferon (IFN) subtypes alpha and beta share a common multicomponent, cell surface receptor and elicit a similar range of biological responses, including antiviral, antiproliferative, and immunomodulatory activities. However, alpha and beta IFNs exhibit key differences in several biological properties. For example, IFN-beta, but not IFN-alpha, induces the association of tyrosine-phosphorylated receptor components ifnar1 and ifnar2, and has activity in cells lacking the IFN receptor-associated, Janus kinase tyk2. To define the structural basis for these functional differences we produced human IFN-beta with point mutations and compared them to wild-type IFN-beta in assays that distinguish alpha and beta IFN subtypes. IFN-beta mutants with charged residues (N86K, N86E, or Y92D) introduced at two positions in the C helix lost the ability to induce the association of tyrosine-phosphorylated receptor chains and had reduced activity on tyk2-deficient cells. The combination of negatively charged residues N86E and Y92D (homologous with IFN-alpha8) increased the cross-species activity of the mutant IFN-betas on bovine cells to a level comparable to that of human IFN-alphas. In contrast, point mutations in the AB loop and D helix had no significant effect on these subtype-specific activities. A subset of these latter mutations did, however, reduce activity in a manner analogous to IFN-alpha mutations. The effects of these mutations on IFN-beta activity are discussed in the context of a family of related ligands acting through a common receptor and signaling pathway.

Amino Acid Sequence↗
PubMed1994

[Prehospital circumstances in cardiac arrest. A prospective study from Slagelse].

The circumstances surrounding the patients with cardiac arrest admitted to a Danish Central Hospital are presented. The study was performed as a prospective study. All patients admitted with cardiac arrest during one year to the Central Hospital in Slagelse were followed. One hundred and twenty-four patients were admitted. Sixty (51.2%) were dead on arrival to the Emergency Center and were not rescucitated. 60 patients received rescucitative treatment in the Emergency Center but 31 (25%) did not respond. Twenty-nine patients (23.4%) were primarily rescucitated. Sixteen patients were discharged alive from the hospital. We found a clear effect of a sufficient treatment during the transportation on the mortality rate. There was a borderline difference between the survival of the patients if a doctor was present at the scene of accident or during transportation. Treatment given by laymen at the scene of accident, and the transportation time per se did not influence the mortality.

Adult↗
PubMed1993

1992 Federal Nursing Service Award recipient. An introduction to monoclonal antibody concepts for the oncology nurse.

Over the past 16 years, there has been an explosion of knowledge in the field of monoclonal antibody technology. There is an enormous potential for the use of monoclonal antibodies in the diagnosis and treatment of malignancies, and many clinical trials are under way. This paper serves as an introduction to monoclonal antibody concepts for the oncology nurse. Antibody/antigen principles, production of monoclonal antibodies, common side effects of use, and nursing implications are discussed. Results of a recent clinical trial and a patient case study are used to illustrate the application of radiolabeled monoclonal antibodies as a cancer diagnostic technique.

Antibodies, Monoclonal↗
PubMed1992

EDTA treatment of intermittent claudication--a double-blind, placebo-controlled study.

A double-blind, randomized multicentre study was undertaken to evaluate the possible effect of chelation treatment with ethylenediamine-tetraacetic acid (EDTA) in patients with severe intermittent claudication. A total of 153 patients received 20 intravenous infusions of either 3 g Na2EDTA or placebo during a period of 5-9 weeks. Vitamin, mineral and trace element supplements were administered orally. The changes observed in the pain-free and maximal walking distances, measured on a treadmill, were similar in the two groups. During the 3-month (n = 149) and 6-month (n = 123) follow-up period, no long-term therapeutic effect of EDTA could be demonstrated. The ankle-brachial blood pressure index remained unchanged throughout the study period. This study failed to demonstrate any effect of EDTA chelation treatment in intermittent claudication.

Aged↗
PubMed1991

[Temporary pacing in connection with anesthesia. Indications and methods].

The indications for permanent pacing are, by and large, established. Considerable uncertainty still exists about when temporary pacing should be initiated not least in connection with anaesthesia and surgery. The indications for temporary pacemaker treatment are reviewed and, in addition, the two methods of temporary pacemaker treatment are discussed, viz the transvenous and the transcutaneous method.

Anesthesia, General↗
PubMed1991

Murine tumor cells expressing the gene for the human interferon alpha beta receptor elicit antibodies in syngeneic mice to the active form of the receptor.

The cellular receptor for the human alpha and beta interferons (IFN) was expressed, by gene transfer, in a murine hepatoma-derived cell line, BTG 9A. Injected subcutaneously into the syngeneic mouse (C57BL/6), the parental and the transfected cells grew and formed tumors which later regressed. More than half the mice bearing tumors derived from cells expressing the receptor, developed IgG antibodies capable of blocking the activity, on human cells, of human recombinant IFN-alpha B, -alpha A, -alpha D and of natural human IFN-beta, but not of recombinant IFN-gamma. Cross-reactivity of human IFN-alpha on murine and bovine cells was unaffected by these antibodies. The binding of human IFN-alpha to solubilized receptors from human lymphoid cell lines was also blocked and complexes of radiolabeled recombinant IFN-alpha A or IFN-alpha B, chemically cross-linked to their human receptor could be immunoprecipitated by the antisera. IFN alpha beta receptor protein, purified by electrophoresis in sodium dodecylsulfate, was not recognized. We conclude that the antibodies are directed against the forms of the IFN alpha beta receptor actually expressed on the membrane.

Animals↗
PubMed1991

[Working and living conditions of Danish medical assistant].

In spring 1988 almost 6,000 members of the Danish Association of Young Doctors answered a questionnaire containing 132 questions about their working and living conditions. The Danish study shows that many young doctors are worried by uncertain conditions of employment, lack of opportunities for further training, and demanding emergency duty. Traditional sex role patterns in the families afflict the women doctors who have difficulty in holding their own in the keen competition. Stress in different forms is regarded as a problem by about 25 per cent. Almost half of the respondents had wholly or partially regretted their choice of a career and one fifth had considered changing their profession. The physicians own occupational injuries are substantially (24 per cent) underreported. Swedish and Finnish studies indicate that the problems in the medical profession are similar in the Nordic countries.

Alcohol Drinking↗
PubMed1990

[Acute iliofemoral venous thrombosis. 26 cases treated with thrombectomy, temporary arteriovenous fistula and anticoagulants].

During a period of two years, 26 patients with acute iliofemoral venous thrombosis were treated with venous thrombectomy, establishing of a temporary arteriovenous fistula and anticoagulants as thrombolytic treatment was not possible. Twenty-five patients were followed-up after an average of 20 months with clinical examination, phlebography, venous strain-gauge pletysmography and vein-pump examination. At follow-up examination, 68% of the patients were free from symptoms, in 54% vein-pump function was normal and 79% had normal venous strain-gauge pletysmographic findings. One patient had venous claudication. Thrombectomy with temporary arteriovenous fistula formation offers a possibility of ensuring normal venous drainage from the lower limbs in patients with contraindications to thrombolysis.

Acute Disease↗
PubMed1990

Hydrophobic cluster analysis reveals duplication in the external structure of human alpha-interferon receptor and homology with gamma-interferon receptor external domain.

Evidence is presented, based on sequence comparison according to Hydrophobic Cluster Analysis, of a structural and evolutionary relationship between the human alpha/beta-interferon receptor and the human and mouse gamma-interferon receptor. These results predict that the human alpha/beta-interferon receptor extracellular part is organised in two homologous subdomains connected by a proline linker. They also predict that both subdomains present some homologies to the external domain of mouse and human gamma interferon receptor.

Amino Acid Sequence↗
PubMed1990

[The significance of the environment for delivery at a special department].

A material of 292 normal pregnant women was subdivided at random for delivery either in the traditional delivery room or a newly established environmental room. The alterations in the physical environment of delivery did not result in more numerous spontaneous deliveries. A high frequency of episiotomies, stimulation of contractions and artificial deliveries in primiparae occurred particularly when it is considered that two thoroughly investigated groups of low-risk pregnant women were concerned. However, qualitative differences in the experience of the course of delivery were found in favour of the environmental room. The quality of the experience of delivery did not depend so much on the physical environment but more on contact with the staff members responsible for conduct of the delivery.

Delivery, Obstetric↗
PubMed1989

Thrombectomy of acute iliofemoral venous thrombosis during pregnancy.

Acute iliofemoral venous thrombosis is six times more frequent among pregnant than nonpregnant women. The disease is serious because of the risk of pulmonary embolism. The treatment of choice with regard to prevention of post-thrombotic disorders is controversial. Pregnant women represent a therapeutic problem because thrombolysis is hazardous to the fetus. We have performed thrombectomy and temporary arteriovenous fistula in combination with anticoagulant treatment upon all pregnant or recently pregnant women coming to our department with acute iliofemoral venous thrombosis from August 1985 to March 1987. In this period, eight pregnant women and one woman 12 days after cesarean section were admitted. This is an incidence of 0.83 per 1,000 deliveries. All of the women were evaluated with a mean follow-up time of 15 months. Six of nine patients were clinically asymptomatic. Six had a normal ambulatory strain gauge volumetry and seven, a normal occlusion strain gauge plethysmography. Two women delivered by cesarean section, one woman had an induced abortion because of the disease, two women delivered after partus provocatus and four delivered spontaneously. We conclude that thrombectomy and temporary arteriovenous fistula together with heparin is a safe treatment of acute iliofemoral venous thrombosis during pregnancy. There is no need to interrupt the pregnancies except for obstetric reasons.

Adult↗
PubMed1985

Enterobius vermicularis and acute appendicitis.

The relationship between Enterobius vermicularis and acute appendicitis has been discussed for more than 80 years. The latest histological examinations show that the parasites do invade the wall of the vermiform appendix, and related to these are inflammatory reactions. This invasion causes the symptoms that lead to appendectomy. A case report of a patient with symptoms of acute appendicitis and a histological finding of intramural parasites is presented.

Acute Disease↗