Biomedical subjects

K Momoeda

Publications and source records attributed to K Momoeda.

5 recordsLinked to original sources

PubMed1996

Sensitive method for the determination of pulmonary surfactant phospholipid/sphingomyelin ratio in human amniotic fluids for the diagnosis of respiratory distress syndrome by thin-layer chromatography-immunostaining.

By TLC-immunostaining with the monoclonal antibody VJ-41, which preferentially reacted with sphingomyelin (Sm) and disaturated fatty acid-containing phosphatidyl choline (DSPC), Sm and surfactant phospholipid dipalmitoyl PC were only detected in the lipid extracts from human amniotic fluid. The method was useful in the selective and simultaneous determination of surfactant phospholipid and Sm concentrations in the amniotic fluids to determine the level of maturity of the lungs of the fetus. By measuring the density of spots visualized by TLC-immunostaining, we detected Sm at a sensitivity two times higher than that for dipalmitoyl PC using the antibody. More than 50 ng of dipalmitoyl PC and Sm was detected on the same TLC plate and the standard curves were linear up to 1 microgram of phospholipids. The method was applied to determine the surfactant phospholipid/Sm ratio in 20 microliter of the amniotic fluids obtained at delivery, and the amniotic fluids from the women who delivered a baby suffering from respiratory distress syndrome (RDS) were easily discriminated from the normal amniotic fluids. In an analysis of 200 microliter of amniotic fluids from 4 RDS cases and 16 normal baby cases, the mean DSPC/Sm ratios were 0.97 +/- 0.53 and 5.75 +/- 1.29, respectively.

Amniocentesis↗

PubMed1996

Developmental changes of neutral glycosphingolipids as receptors for pulmonary surfactant protein SP-A in the alveolar epithelium of murine lung.

A dramatic change in the glycosphingolipid composition in murine lung occurred between 1 day and 1 week after birth. GlcCer and LacCer were the predominant neutral glycosphingolipids prenatally and 1 day after birth, and the concentrations of globo- and ganglio-series glycosphingolipids increased abruptly from 1 week after birth, reaching maxima at 2-3 weeks. To explore the functional significance of the change, we examined the role of neutral glycosphingolipids as receptors for the murine pulmonary surfactant protein, SP-A, and found that SP-A bound to LacCer, Gg3Cer, and Gg1Cer, but not to Gb3Cer, Gb4Cer, IV3GalNAc alpha-Gb4Cer, sulfatide, or several gangliosides. On TLC-blotting with 125I-labeled SP-A, the binding of SP-A to Gg3Cer and Gg4Cer was 5 times higher than that to LacCer, and on immunohistochemical staining Gg4Cer and Gg3Cer was mainly observed in the alveolar epithelium. Thus, the capacity to retain SP-A of glycolipid receptors per gram dry weight of lung at 1 week after birth was 1.6 times higher than that at 1 day after birth, and reached a maximum 3 weeks after birth. These findings suggest that the enhanced synthesis of the ganglio-series neutral glycosphingolipids 1 week after birth results in an increase in the binding capacity for SP-A on the epithelial cell surface of alveoli.

Animals↗

PubMed1995

Characteristic expression of GD1 alpha-ganglioside during lactation in murine mammary gland.

Cellular proliferation and differentiation in mammary gland are known to be significantly altered during pregnancy and subsequent lactation. To characterize the different stages of mammary gland during pregnancy and lactation, we analyzed the glycosphingolipid compositions in the mammary gland of DDD and ICR mice at several periods of pregnancy and lactation, and found that the ganglioside composition, but not neutral glycosphingolipids, was characteristically altered during the pregnancy and lactation periods. The concentrations of acidic glycosphingolipids, I3SO3-GalCer 1, GM3, GM1a, GM1b and GD1a, were reduced during the course of pregnancy and lactation. GD1 alpha (III6NeuAc alpha,IV3NeuAc alpha-Gg4Cer) was first detected at the mid-period of pregnancy (12 days of pregnancy for ICR mice), increased in concentration at the late-period of pregnancy (GD1 alpha concentration was 100 times higher at 18 days than that at 12 days of pregnancy), and was a major ganglioside comprising 60-70% of the total lipid-bound sialic acid in the mammary gland of ICR and DDD mice at the lactation period, indicating that expression of GD1 alpha is associated with the lactating mammary gland of mice. In fact, GD1 alpha was highly concentrated in the milk fat globule, in which it was a major component.

Animals↗

PubMed1995

Alteration in the reactivity of sphingomyelin in mitogen-stimulated lymphocytes.

The antigens for a monoclonal antibody, VJ-41, established by alloimmunization of B10.A(3R) mice with lymphocytes from B10.A(5R) mice and screening of its reactivity toward Con A-stimulated human T lymphocytes, were found to be phosphorylcholine-containing ceramides (sphingomyelin) and disaturated fatty acyl glycerol (phosphatidyl-choline, PC), but neither deacylated sphingomyelin nor unsaturated fatty acid-containing PC reacted with the antibody. Although the reactivity of disaturated fatty acyl PC increased with increasing chain length, that of sphingomyelin was stronger than that of di-20:0-PC. The binding of the antibody to Con A-stimulated lymphocytes was inhibited by sphingomyelin-containing liposomes, but not by di-18:0-PC-containing ones, and the concentration of sphingomyelin in Con A-stimulated human T-lymphocytes was the same as that in non-stimulated ones, indicating that the reactivity of sphingomyelin in lymphocytes is altered by Con A-stimulation.

Animals↗

PubMed1995

[Anesthesia for SUMO wrestlers].

We anesthetized 34 sumo wrestlers. All patients were male. Average age was 21.1. Their height was 181.9 +/- 4.5 (mean +/- SD) cm, and they weighted 135.2 +/- 16.9 kg. Anesthetic methods used were spinal anesthesia alone in 17 patients, general anesthesia alone in 9, general anesthesia + epidural anesthesia/or spinal anesthesia/or axillary block in 8. The preoperative data showed findings common to obese patients; liver dysfunction, abnormal blood sugar, and abnormal ECG such as ST depression or left ventricular hypertrophy. For intubation, fiberscopy was required in one patient, and almost all patients required high FIO2, otherwise SaO2 decreased. For spinal anesthesia or epidural anesthesia, we needed long needles of 8 to 15 cm.

Adult↗