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K Mortensson-Egnund

Publications and source records attributed to K Mortensson-Egnund.

10 recordsLinked to original sources
PubMed1989

[Rotavirus in stools from hospitalized children].

Electron microscopy, ELISA for rotavirus and virus cultivation in cell cultures were used to study the occurrence of rotavirus in 451 fecal samples collected from hospitalized children. Rotavirus was found in samples from 24 of 27 patients admitted with acute diarrhoea. All specimens from randomly selected patients without diarrhoea were negative. Two patients developed nosocomial rotavirus infection. ELISA was positive, but electron microscopy was negative in two specimens obtained from asymptomatic newborns. In this investigation rotavirus was the dominating agent isolated in infectious gastroenteritis. We found no rotavirus excretors without diarrhoea.

Child↗
PubMed1986

Improved ELISA for the detection of adenovirus antigen in faeces extracts by the biotin/streptavidin interaction.

Two enzyme-linked immunosorbent assays for detection of adenovirus antigen in faecal extracts have been established. A conjugate of rabbit anti-(human) adenovirus immunoglobulin and horseradish peroxidase (HRP) prepared by means of a hetero-bifunctional reagent, N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), was used in the conventional ELISA, and a biotin-labelled antibody in combination with a streptavidin/peroxidase complex in the other (BS-ELISA). A collection of 60 faecal extracts, in which adenovirus had been demonstrated by immunosorbent electron microscopy (ISEM) in 29 samples, was selected and examined by ELISA and BS-ELISA. The presence of adenovirus was demonstrated in 38 (63%) of the samples by BS-ELISA compared to 34 (58%) by ELISA, showing the advantage of the biotin/streptavidin system for setting up ELISA assays. Comparison of the three different techniques showed that the biotin/streptavidin (BS-ELISA) is the most sensitive assay for detection of adenovirus antigen in faecal samples.

Adenoviruses, Human↗
PubMed1982

Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples.

One hundred and fifteen faecal samples from children with gastroenteritis have been examined by solid-phase immune electron microscopy (SPIEM), standard direct electron microscopy and enzyme-linked immunosorbent assay (ELISA). Diluted high-titered anti-(human) rotavirus serum, produced in rabbit by immunization with purified pooled rotaviruses from gastroenteritis patients, was used as antiserum in the immunological tests. The presence of rotavirus was demonstrated in 41 (36%) of the 115 samples by both SPIEM and ELISA. No false positive reactions were seen. By direct electron microscopy rotavirus was detected in 35 (30%) of the specimens. In addition, other gastroenteritis viruses like adeno-, astro- and calicivirus were visualized by this non-specific test in eight samples. The applicability of these three methods in routine diagnostic work is discussed.

Antigen-Antibody Reactions↗
PubMed1969

Alpha-L-fucosidase from a soil bacterium.

Intracellular glycosidases were measured in cell-free extracts obtained by ultrasonic disruption of a gram-negative soil coccobacillus (Chase, 1938). From these extracts, alpha-l-fucosidase was purified about 120-fold by salting out with (NH(4))(2)SO(4), ion exchange chromatography, and gel filtration. The approximate molecular weight of the enzyme was 50,000; its pH optimum was 5. The enzyme was inhibited by l-fucose and split this sugar from a purified acid mucopolysaccharide from chicken chorioallantoic fluid. The acid mucopolysaccharide is identical with a component (host antigen) of the hemagglutinin of influenza virus. Its antigenic reactivity is altered by cell-free extracts of the bacterium, in which the responsible enzyme is thought to be an alpha-l-fucosidase.

Bacteria↗