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Biomedical subjects

K Motomatsu

Publications and source records attributed to K Motomatsu.

At least 19 recordsLinked to original sources
PubMed1996

Aqueous flare in eyes with senile disciform macular degeneration: correlation with clinical stage and area of neovascular membrane.

BACKGROUND: The aim of the present study was to investigate the aqueous flare in eyes with senile disciform macular degeneration (SDMD), divided into different clinical stages, and the correlation between the aqueous flare and the area of the neovascular membrane. METHODS: Eighty-six eyes of 44 patients with SDMD were examined using a laser flare meter. The area of the neovascular membrane was measured by means of a digitizer in images obtained using indocyanine green videoangiography. RESULTS: The mean value of the aqueous flare was 5.91 +/- 2.51 (photon count/ms) in 7 predisposing stage eyes, 5.68 +/- 1.64 in 15 initial stage eyes, 9.09 +/- 7.65 in 24 advanced form eyes, 5.40 +/- 1.42 in 11 disciform scar eyes, and 5.36 +/- 1.72 in 29 fellow eyes. The aqueous flare value was significantly (P < 0.01) higher in eyes with the advanced form of SDMD than in the fellow eyes. There were no significant differences in aqueous flare values between eyes with predisposing stage, initial stage, and disciform scar and fellow eyes. The aqueous flare value increased significantly with increasing area of neovascular membrane (R = 0.68, P < 0.01). CONCLUSION: The present results suggest that the aqueous flare increases with increasing neovascular membrane area in eyes with SDMD, and decreases with scarization of the neovascular membrane.

Aged↗
PubMed1988

Three-dimensional structure of endothelial cells in hepatic sinusoids of the rat as revealed by the Golgi method.

The three-dimensional structure of endothelial cells in the hepatic sinusoids of the rat was studied by application of light- and electron microscopy on Golgi-impregnated specimens. A number of endothelial cells could thus be individually delineated throughout the hepatic lobules. The cytoplasm, showing heavy silver deposits, consists of two distinct areas, a thick and thin portion. The thick portion, issuing from the region of the perikaryon, branches and tapers toward the cell periphery. The thin portion, occupying the remainder of the cytoplasm, consists largely of highly fenestrated sieve plates. Some intralobular variation can be noted; the thick portion of the endothelial cells is well developed in the periportal zone, while the cells in the centrilobular zone are relatively rich in thin portions. In addition, the area of distribution of an individual endothelial cell is larger in the centrilobular sinusoids than in the periportal zone. Some endothelial cells also possess unique cytoplasmic processes projecting into the intercellular space between hepatocytes and connecting the sinusoidal walls of neighboring sinusoids. These processes may anchor the endothelial cells to the hepatic plates.

Animals↗
PubMed1987

Stellate cells storing retinol in the liver of adult lamprey, Lampetra japonica.

Distribution, localization and fine structure of the stellate cells in the liver of lamprey, Lampetra japonica, were studied during the spawning migration by use of Kupffer's gold-chloride method, fluorescence microscopy for vitamin A (retinol) and electron microscopy. The stellate cells in the lamprey liver differ in some of their properties from those in mammalian livers. Stellate cells which store abundant retinol in lipid droplets, occur not only in the hepatic parenchyma, but also in the dense perivascular and capsular connective tissue of the liver and in the interstitium of pancreatic tissue. In the hepatic parenchyma these cells are located perisinusoidally or along thick bundles of collagen fibrils. The stellate cells display a number of large retinol-containing lipid droplets, granular endoplasmic reticulum, tubular structures, dense bodies. Golgi complex, microtubules, and microfilaments. In the space of Disse, the stellate cells and extracellular fibrilar components such as collagen fibrils and microfibrils (11-12 nm in diameter) are intervened between the two layers of basal laminae. Differentiation and possible functions of the stellate cells in the lamprey liver are discussed.

Animals↗
PubMed1986

Improved Kupffer's gold chloride method for demonstrating the stellate cells storing retinol (vitamin A) in the liver and extrahepatic organs of vertebrates.

This paper describes our modification of the classical gold chloride technique for the demonstration of the perisinusoidal stellate cells in the liver. The results of the method as introduced by von Kupffer (1876) are unpredictable. Using our modification, high quality gold preparations can be obtained. The method allows selective staining of retinol (vitamin A)-storing stellate cells in the liver and extrahepatic organs of various vertebrates. The sensitivity of the reaction is comparable to that of the fluorescence method for retinol. The technique is simple and the preparations keep for several years. Formol fixed specimens can be counterstained with Sudan III or hematoxylin. We have also developed a simple technique for making "sinusoid-net preparations," removing the parenchymal cells by supersonication. The clear visualization of the stellate cells that results has made it possible to study the distribution of these cells.

Animals↗
PubMed1984

Discrepancy in the thrombolytic effect of UK between angiographic and circulatory evaluations.

A clinical case in which angiographic and circulatory evaluations of the thrombolytic effect of urokinase (UK) were not correlated, is described. Circulatory improvement distal to the thrombosis was recognized on the basis of a rise in skin temperature and increased palpability of the pulse at the distal artery. However, angiography revealed the development of thrombosis. This discrepancy may have arisen from the hypotensive action of UK preparations due to increased flow in the collateral circulation. The significance of angiography in providing direct evidence for assessing the thrombolytic effect of UK is emphasized.

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