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Biomedical subjects

K Nilsson

Publications and source records attributed to K Nilsson.

At least 451 records · Page 25Linked to original sources
PubMed1974

Density gradient separation of lymphoid cells adhering to protein-A-containing staphylococci.

A cell separation technique was designed based on the interaction between cell-surface-bound IgG and protein A of Staphylococcus aureus. The density of lymphoid cells coated with IgG antibodies against one of the surface markers was increased by adherence of staphylococci. Cells with adhering bacteria were separated from cells without bacteria by density gradient centrifugation in 11.5% sodium metrizoate. Bacteria were removed from the lymphoid cells by lysostaphin digestion. The purity of separated cells was approximately 95% even when the proportion of a specific cell population was below 10% in the initial mixture. The viability and the ability of cells to multiply in vitro were not significantly impaired by the fractionating procedure. The technique can generally be applied for cell separation, provided antibodies of the IgG class against specific surface markers are available.

Antigen-Antibody Reactions↗
PubMed1974

Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas.

Three exceptional cell lines have been tested for the presence of the Epstein-Barr virus genome by nucleic acid hybridization (complementary RNA.DNA) and Epstein-Barr virus-determined nuclear antigen tests. Two lines were derived from Swedish lymphoma cases and one from an African Burkitt-like lymphoma biopsy that was negative for Epstein-Barr virus DNA and the virus-determined nuclear antigen. All three lines apparently lacked the viral genome. Two of the three lines clearly had characteristics of B-cells (bone-marrow-derived).

Antigens, Viral↗