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K Oates

Publications and source records attributed to K Oates.

45 records · Page 3Linked to original sources

Further observations on Calliacantha Leadbeater Choanoflagellata), with special reference to C. simplex sp. nov. from many parts of the world.

C. simplex is a new species with an unusually wide geographical range, occurring at sea temperatures as varied as 0 degrees C under ice at Pt Barrow or -1 degrees C in arctic Canada, to 22 degrees C on the equator at the Galapagos Islands. The morphology and range of variation are illustrated by means of light microscopy, transmission electron microscopy and scanning electron microscopy applied to dry whole mounts prepared in situ from freshly-gathered wild material. Among the diagnostic structural features, special interest attaches to the position of the anterior transverse costa which is located unusually far back in comparison with other members of the genus; it is also shown to be within and not outside the ring of four longitudinal costae building up the lorica chamber, although this feature can only be ascertained by scanning. The differences between this species and C. natans (type species of the genus) are shown to be greater and to involve more characters than could previously have been recognized, and some possible functional implications are discussed in a preliminary way.

Animals↗

Electrolyte levels in neoplastic and nonneoplastic human urothelium after ouabain treatment: X-ray microanalysis of bulk hydrated samples.

Samples of neoplastic and nonneoplastic human urothelium were immediately frozen, incubated in Krebs' saline and then frozen, or incubated in 10(-5) mol/L ouabain in Krebs' saline and then frozen. The frozen specimens were then planed in a cryoultramicrotome and examined by low-temperature scanning electron microscopy. X-Ray microanalysis was performed on the superficial urothelial cells. Neoplastic cells immediately frozen and those incubated in Krebs' saline had significantly higher K+/Na+, K+/P, and K+/Cl- ratios and lower Na+/P and Cl-/P ratios than nonneoplastic cells. Incubation in ouabain led to a fall in the K+/Na+, K+/P, and K+/Cl- ratios and a rise in the Na+/P and Na+/Cl- ratios in both neoplastic and non-neoplastic cells and effectively nullified the difference between them. These results are consistent with the concept that in neoplasia a primary event is stimulation of Na+/H+ exchange, which leads to secondary stimulation of the ouabain-sensitive Na+/K+ ATPase pump.

Adult↗

Impact of confocal scanning optical microscopy on pathological practice.

This review outlines some recent advances likely to influence the hospital laboratory practice of light microscopy, a technique that remains the cornerstone of all surgical pathology. The principal advances are confocal scanning optical ('indirect view') microscopy, to which this short account is restricted, and tandem scanning ('direct view') microscopy.

Histological Techniques↗