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Biomedical subjects

K Olczyk

Publications and source records attributed to K Olczyk.

At least 19 recordsLinked to original sources
PubMed1996

Glycosaminoglycans of human serum and their alterations in diabetes mellitus.

Human serum contains several glycosaminoglycans (GAGs), mainly chondroitin sulphates and significantly less of heparan sulphate + heparin and dermatan sulphate. The non-insulin-dependent diabetes mellitus (with vascular complications) was associated with a significant increase in total serum GAG concentration, mainly of chondroitin sulphates and dermatan sulphate, with a simultaneous decrease in heparan sulphate + heparin level. These alterations were much more evident in patients with poor metabolic control. Hyaluronic acid (undetectable in healthy subjects and in patients with good metabolic control) appeared only in trace amounts in poorly controlled diabetic individuals. The obtained data allow to conclude that the diabetes mellitus-associated disturbances in tissue GAG metabolism lead to significant alterations in serum GAG composition.

Aged↗
PubMed1994

The lipid peroxidation products and the enzymes of antioxidant system in patients with diabetes mellitus.

The lipid peroxidation product level (conjugated dienes, malonyldialdehyde) as well as superoxide dismutase and glutathione peroxidase activity in diabetic patients were studied. It was found that diabetes mellitus is accompanied by an increase in serum conjugated diene and malonyldialdehyde level as well as by an increased malonyldialdehyde concentration in red blood cells. No changes in erythrocyte superoxide and glutathione peroxidase activity in diabetic patients were found. There were also no differences between diabetic patients with poor and good metabolic control in any of the examined parameters.

Adult↗
PubMed1994

Influence of chronic mercury poisoning upon the connective tissue in rats. I. Effect of mercuric chloride on glycosaminoglycan levels in tissues, serum and urine.

Rats were intoxicated with mercuric chloride (1mg/kg b.w.) daily, for 12 weeks. A decrease in total glycosaminoglycan content was shown in the skin, the lungs, the liver and the heart muscle. These changes were accompanied by a slight alteration of the glycosaminoglycan pattern, a decrease in hyaluronic acid in the skin, the lungs and the heart muscle and an enhancement of heparan sulphate level in the kidneys. In serum of mercury-intoxicated rats, an increase in total glycosaminoglycan levels was shown. This enhancement was caused by elevation of almost all fractions. Urine output of glycosaminoglycans was higher in mercury-treated animals as compared to the controls.

Animals↗
PubMed1994

Influence of chronic mercury poisoning upon the connective tissue in rats. II. Effect of mercuric chloride on collagen and elastin.

Rats were intoxicated with mercuric chloride (1 mg/kg b.w.) daily, for 12 weeks. An increase of total collagen and elastin content was found in the skin, the lungs, the liver, the kidneys and the heart muscle. The increase resulted from the elevated level of soluble collagen. These changes were accompanied by elevated hydroxyproline level in serum and urine. It is concluded that chronic intoxication with mercury leads to disturbed composition of the connective tissue matrix.

Animals↗
PubMed1994

Changes in macromolecular components of prolapsed intervertebral discs.

Significant quantitative differences between macromolecular components of the nuclei pulposi of prolapsed human intervertebral discs and those taken at necropsy were found. The nuclei pulposi of prolapsed discs contained about twice more collagen in comparison to control tissues. The amount of type I and type III collagens grew in a higher degree in comparison to type II of this protein. A distinctly higher solubility of the prolapsed discs collagen, together with an increased aldehyde content in solubilized collagen fractions were observed. A distinct decrease in the amount of glycosaminoglycans, especially CH-4-S and CH-6-S were found. Furthermore, a significant increase in elastin content and its susceptibility to the proteolytic action of elastase were found. The significance of these phenomena for pathobiochemistry of disc prolapse is discussed.

Adult↗
PubMed1994

Age-related changes of elastin content in human intervertebral discs.

It was found that the ageing process is accompanied by significant changes of elastin content in human intervertebral discs. In the case of annuli fibrosi only a slight age-related increase during the first four decades of life followed by a decrease in later periods of life were observed. In the case of nuclei pulposi the changes were much more apparent. A systematic decrease in elastin: collagen ratio was observed, especially in the case of nuclei pulposi. An increase in elastin: GAGs ratio was observed during the first 4 decades of life. After that period no further changes of the ratio were found.

Adolescent↗
PubMed1993

Age-related changes in glycosaminoglycans of human intervertebral discs.

The age-related changes in glycosaminoglycan composition of human intervertebral discs were studied. Large number of tissue samples taken from 301 individuals at autopsy or at operation of prolapsed discs was investigated. It was found that the ageing process is accompanied by a progressive decrease in GAG content, both in annulus fibrosus and in nucleus pulposus. The GAG: collagen ratio significantly decreases. The KS and Ch-6-S were found to be the most abundant GAGs in both parts of the disc and their amounts decrease during the ageing. A distinct, age-related increase in KS:Ch-S and Ch-6-S:Ch-4-S ratios was observed. Significant quantitative differences between the GAG contents in normal and prolapsed discs were found. The nuclei pulposi of prolapsed discs contained less GAGs, especially KS and Ch-6-S in comparison to normal tissue of the same age. Furthermore, the age-related decline of GAGs:collagen ratio was more distinct in the prolapsed discs. It seems that these differences in GAG composition may be important in the mechanism of predisposition to discs prolapse.

Adolescent↗
PubMed1993

Matrix-degrading lysosomal hydrolases in serum of rats with hepatic fibrosis treated with methylthiouracil or thyroideum.

Thyroid hormones or methylthiouracil (MTU) were given to healthy rats or animals with hepatic fibrosis. Administration of thyroid hormones to rats with carbon tetrachloride-induced hepatic fibrosis caused an increase in serum activity of matrix-degrading hydrolases although the increase was lower than that in healthy animals treated with thyroid hormones only. Methylthiouracil administration had no effect on the enzyme activity in healthy rats and resulted in a decreased activity in rats with hepatic fibrosis.

Animals↗
PubMed1992

Age-related changes in collagen of human intervertebral disks.

Age-related changes of human intervertebral disk collagen have been investigated. A progressive, age-related accumulation of collagen, both in annulus fibrosus and in nucleus pulposus was found. A decrease in collagen solubility was observed during physiological aging. Up to the 3rd-4th decades of life, a significant increase in the aldehyde content in disk collagen has been observed. Nuclei pulposi of the prolapsed disk contain more collagen, and its solubility was higher in comparison to corresponding necropsy material. Some alterations in the collagen of prolapsed disks may be considered as symptomatic of accelerated aging.

Adolescent↗