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K Owens

Publications and source records attributed to K Owens.

45 records · Page 3Linked to original sources
PubMed1970

Lipids of dystrophic and normal mouse muscle: whole tissue and particulate fractions.

Myofibrillar, mitochondrial, and microsomal fractions were prepared from normal and dystrophic mouse limb muscle by differential centrifugation and analyzed for phospholipids and cholesterol. Fatty acids and aldehydes of neutral lipids and of phospholipids from whole muscle and particulate fractions were also determined. Normal microsomes contained more lecithin and less total ethanolamine phospholipids and cardiolipin than mitochondria. The myofibrils had an intermediate phospholipid composition, but their cholesterol-phospholipid ratio was smaller than that of the other two fractions. Except for an increased percentage of phosphatidalethanolamine in the dystrophic mitochondria, only the composition of the dystrophic microsomes differed from normal by containing less lecithin but more total ethanolamine phospholipid, phosphatidalethanolamine, sphingomyelin, and cholesterol. No significant differences were found in the fatty acid composition of neutral lipid extracts from normal and dystrophic preparations, but there was a significant decrease in the percentage of 22:6 in phospholipids from both dystrophic whole muscle and microsomes (-25% and -37%, respectively), whereas the 20:4 content was unaltered. By contrast, the percentages of 18:0 and total fatty aldehyde increased significantly. Phospholipid extracts from all dystrophic samples showed a significant decrease in 16:0 and an increase in 18:1 as compared with the normal.

Aldehydes↗
PubMed1966

Chromatographic behaviour of rat-liver monophosphoinositide.

1. Chromatography of rat-liver lipids on a column of silicic acid or a mixture of silicic acid and Hyflo Super-Cel, with chloroform-methanol mixtures, gave monophosphoinositide-containing fractions which were invariably contaminated by the presence of nitrogen-containing phospholipids. The behaviour of the inositide was extremely sensitive to column loading and the results with different batches of silicic acid were not reproducible. 2. However, when chromatography on an alumina column was used, the solvent system chloroform-methanol-water (23:23:4, by vol.) completely eluted the neutral lipids, choline-containing phospholipids and phosphatidylethanolamine. An increase of the water content of the solvent to 14% (by vol.) then led to the elution of the monophosphoinositide component, now free from nitrogen-containing phospholipids, but still contaminated by the presence of a phospholipid, which from its properties was taken to be polyglycerophosphatide. 3. Most of the polyglycerophosphatide could be removed from a rat-liver lipid extract by silicic acid chromatography with chloroform-methanol (19:1, v/v). The other phospholipids were then eluted and applied to an alumina column, whereby a monophosphoinositide fraction of much greater purity was obtained. 4. Further purification of the monophosphoinositide was achieved by chromatography on a mixture of silicic acid and cellulose powder. The final product was virtually pure by thin-layer chromatography and gave the expected analysis for monophosphoinositide.

Animals↗
PubMed1966

A two-dimensional thin-layer chromatographic procedure for the estimation of plasmalogens.

1. The use of two-dimensional thin-layer chromatography is described that allows the rapid and simultaneous determination of phospholipid classes and their constituent plasmalogens. 2. The method is based on the specific hydrolysis of plasmalogens to (2-acyl) lysophospholipid in the presence of a mercuric chloride spray reagent. 3. The proportion of mercuric chloride-labile phospholipid present in each phospholipid class, calculated on the basis of phosphorus recoveries from the charred chromatogram, was compared with the proportion of long-chain aldehyde and of total lipid phosphorus found in small-scale preparations of each class of phospholipid. 4. The method permits the determination of individual plasmalogens on preparations containing as little as 0.2mug.atom of total lipid phosphorus.

Animals↗