Biomedical subjects

K Oyaide

Publications and source records attributed to K Oyaide.

3 recordsLinked to original sources

PubMed1989

[Mutagenicity studies of miporamicin].

Mutagenic activity of miporamicin (MPM), a new macrolide antibiotic for animal use, was examined using the reversion test with bacteria, the chromosomal aberration test with mammalian cells in culture and the micronucleus test with rodents. In the reversion test, MPM exhibited severe growth inhibition effect on the test bacteria but caused no increase of revertant colonies over the baseline levels, alone or in combination with S 9 mixture. In the chromosome aberration test, MPM induced a medium grade increase of chromosome aberrations at high concentrations, but induced no increase of polyploid cells over the control level. In the micronucleus test, MPM had no effect on induction of micronucleated polychromatic erythrocyte even at the 1/2 LD50 dose. From these results, we concluded that MPM has no effect on the induction of point mutations but has a weak clastogenicity which is detectable only by in vitro tests.

Animals↗

PubMed1986

[Mutation frequency test for isepamicin (HAPA-B)].

Mutation frequency tests for isepamicin (HAPA-B), a new aminoglycoside antibiotic, were carried out using Salmonella typhimurium strains TA98, TA100, TA1535 and Escherichia coli WP2uvrA. After a 30-minute exposure of bacterial cells at 37 degrees C to HAPA-B in a buffer solution, the cells were washed twice with a buffer solution to minimize the cytotoxicity of the drug. Then washed cells were inoculated on minimum glucose agar plates to determine mutation frequency. The results should no significant increase in mutation frequency with increasing HAPA-B concentration; suggesting that the drug has no mutagenicity detectable by this test system.

Escherichia coli↗

PubMed1986

[Mutagenicity tests for isepamicin (HAPA-B)].

To detect mutagenic activity of isepamicin (HAPA-B), a new aminoglycoside antibiotic, we carried out several mutagenicity tests using microorganisms and cultured mammalian cells. Effects of the antibiotic on DNA-lesion induction and repair were examined using the Rec-assay with Bacillus subtilis and a sister-chromatid exchange test with cultured Chinese hamster cells. The drug caused no increase of mutagenicity indices in both tests, suggesting that the antibiotic has no DNA-damaging effect. To further investigate the effect of HAPA-B on gene-mutation, we performed the Ames Salmonella/microsome plate assay and the thioguanine resistance mutation test using cultured Chinese hamster cells. In both tests, the antibiotic induced no increase of mutation frequencies over spontaneous levels. Clastogenic activities of HAPA-B and its effect on chromosome disjunction were examined by the chromosomal aberration test using cultured Chinese hamster cells. After a short- or long-time exposure, the antibiotic induced neither structural chromosome aberrations nor an increase in the number of tetraploid cells. The negative results from these five test systems with different mechanisms strongly suggest the safety of HAPA-B regarding mutagenicity.

Animals↗