Biomedical subjects

K P Bartholoma

Publications and source records attributed to K P Bartholoma.

4 recordsLinked to original sources

PubMed1976

Study with a multi-threshold HZE-particle dosimeter using plastic detectors.

During the Apollo 16 and 17 missions two units of the Biostack experiment were exposed to cosmic radiation. In this experiment plastic detector sheets were used for recording and tracing the heavy ions. In some of these sheets the integral energy loss spectrum was measured. The measurements were performed in two different cellulose nitrate materials and in Lexan polycarbonate under 4 g cm-2 and 20 g cm-2 absorber thickness. The individual materials have different energy loss thresholds for the registration of heavy ions. The measured number of particles per cm2 with an restricted energy loss REL greater than REL0, follows a power law a REL(b) with b= -2.18 +/- 0.1 while the value of a depends on the exposure time and the absorber thickness. Calculations show that more than 70% of the fluence in the measured REL region is coming from particles with Z> or =20.

Collodion↗

PubMed1974

The charge spectrum of heavy cosmic ray nuclei measured in the Biostack experiment aboard Apollo 16 using plastic detectors.

In the Biostack experiment flown aboard Apollo 16 plastic detectors were used to select those individual biological objects which were hit by single heavy cosmic-ray nuclei during the 266 hours of space flight. The energy loss deposited in the biological object can be determined. This method needs a separate calibration in every experiment, i.e. an individual track etching rate as function of the energy loss. This calibration is given. An area of 36 cm2 of eight cellulose nitrate sheets was scanned for tracks of heavy cosmic-ray particles that stopped in the sheets. About 200 particles could be evaluated. Calibration was achieved using the oxygen isotope 16O of the cosmic-ray ions and the charge spectrum of all measured particles (Z=5-16) could be obtained.

Calibration↗

PubMed1974

Microbial studies in the Biostack experiment of the Apollo 16 mission: germination and outgrowth of single Bacillus subtilis spores hit by cosmic HZE particles.

Bacillus subtilis spores were flown in the Biostack experiment aboard the Apollo 16 command module. The spores embedded in plastic foils were stacked between physical track detectors. The energy loss spectrum of the heavy particles of cosmic radiation was determined. Biological studies were restricted to the high-energy loss component of these particles. Spores that had received single hits whose positions were determined with a typical accuracy of +/- 1 micrometers, were investigated for radiation effects on germination and outgrowth. It was found that germination was not influenced by a hit by an HZE particle, but outgrowth was reduced significantly.

Bacillus subtilis↗

PubMed1973

The Biostack experiment on Apollo 16.

The object of the Biostack experiment is to study the biological effects of high ZE particles of cosmic radiation in order to obtain information on the mechanism of these particles in biological matter. For this purpose individual local evaluation methods have been developed which allow one to identify each biologically effective particle and to correlate the individual hitting particle with the biological effect produced. The Biostack experimental package contains a series of monolayers of selected biological objects (Bacillus subtilis spores, Arabidopsis thaliana seeds, Vicia faba radiculae, Artemia salina eggs) with each layer sandwiched between several different cosmic ion track detectors (nuclear emulsions, cellulose nitrate, polycarbonate). By this arrangement a variety of biological effects due to a single penetrating particle can be analysed. Influence on cellular and tissue development, nuclear damages, and mutation induction are the main investigated effects. These space flight findings will be completed by results of balloon flight and accelerator experiments.

Animals↗