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Biomedical subjects

K P Lander

Publications and source records attributed to K P Lander.

12 recordsLinked to original sources
PubMed1985

Enzyme-linked immunosorbent assay for antibodies to Campylobacter fetus in bovine vaginal mucus.

An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to Campylobacter fetus subspecies venerealis in bovine vaginal mucus. The results of testing 168 samples from experimentally infected, field cases and control cows showed that the ELISA was more sensitive than the vaginal mucus agglutination test and also detected antibodies in earlier stages of infection.

Agglutination Tests↗
PubMed1981

Experimental infection of Rhesus monkeys with a human strain of Campylobacter jejuni.

Young Rhesus monkeys (Macaca mulatta) were infected orally with a human strain of Campylobacter jejuni. The disease induced was mild, with inappetence and diarrhoea of short duration, but prolonged intermittent excretion of the bacteria in the faeces occurred. Bacteraemia was generally present for 2--3 days and later the organisms localized in the liver and gall bladder. Recovered animals, when challenged with the same strain, showed no clinical symptoms, no bacteraemia, and excreted the organisms in the faeces for only 3 days.

Animals↗
PubMed1980

Experimental infection of the bovine udder with Campylobacter coli/jejuni.

Five quarters of the udders of two lactating cows were infected by intramammary inoculation with Campylobacter coli/jejuni in doses ranging from 2.6 colony-forming units (c.f.u.) to 3.8 X 10(9) c.f.u. The infected quarters developed clinical mastitis and the campylobacters were reisolated in large numbers from the milk. The milk from the uninfected quarters, and blood and faeces remained free of the organisms. The campylobacters could only be isolated by incubation of culture plates in a microaerobic atmosphere. The results showed that C. coli/jejuni can cause mastitis in the cow and that the bovine udder is a potential source of C. coli/jejuni in raw milk.

Animals↗
PubMedDate not supplied

The development of a transport and enrichment medium for Campylobacter fetus.

A transport and enrichment medium was developed for Campylobacter fetus. From inocula of between 10 and 35 organisms the medium was able to support the multiplication of 19 of 21 strains of C. fetus if the medium was incubated immediately after inoculation; when incubation was delayed for 3 days after inoculation, only seven of the 21 strains multiplied. From inocula of 100-350 organisms all 21 strains multiplied following immediate incubation, and 20 of 21 when incubation was delayed for 3 days. From inocula of about 10(4) organisms all strains multiplied following immediate or delayed incubation. The medium restricted the growth of Proteus vulgaris and Pseudomonas aeruginosa.

Animals↗
PubMedDate not supplied

The application of a transport and enrichment medium to the diagnosis of Campylobacter fetus infections in bulls.

The use of a transport and enrichment medium (TEM) in the diagnosis of Campylobacter fetus infections in bulls is described. The medium significantly improved the diagnosis rate in samples which, because of the length of time between collection and receipt at the laboratory, were unsuitable for processing by direct culture. The TEM was able to support the viability, and subsequent multiplication, of C. fetus in some samples for up to 7 days before the TEM was incubated. The submission of paired samples of TEM, one containing unfiltered preputial washing (PW) and the other containing PW filtered through a 0.60 micron cellulose acetate filter, significantly increased the accuracy of diagnosis.

Animals↗