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K P Liashchenko

Publications and source records attributed to K P Liashchenko.

At least 19 recordsLinked to original sources
PubMed1991

[The determination of antigen-specific immune complexes by an immunoenzyme method].

The methodological approach permitting the detection of immune complexes containing specific antibodies to a definite antigen in the enzyme-linked immunosorbent assay (ELISA) is described. The basic conditions of the assay were optimized. Immune complexes were precipitated from blood serum with 3.5% polyethylene glycol 6000 for 4 hours. The precipitate thus obtained was dissolved and incubated in polystyrene plates with immobilized antigen at 37 degrees C for a long time (at least 6 hours) in a humid chamber. The amount of bound antibodies, determined by ELISA techniques, was conjectured from the level of antigen-specific immune complexes. The proposed approach can be used in the immunodiagnosis of infectious diseases.

Animals↗
PubMed1991

[The dynamics of the humoral immune response to mycobacterial antigens in mice].

The main regularities of humoral immune response to mycobacterial antigens have been studied in experiments on BALB/c mice immunized with live and thermoinactivated Mycobacterium tuberculosis, var. bovis. As shown in this study, the maximum level of serum antibodies to mycobacterial antigen is achieved in two weeks after immunization irrespective of the dose and viability or mycobacteria, then follows a decrease in the antibody level. The absence of uniformity in the dependence of primary immune response and the formation of immunological memory on the dose and viability of mycobacteria has been demonstrated.

Animals↗
PubMed1991

[The formation of delayed hypersensitivity to mycobacterial antigens].

In experiments on guinea pigs and BALB/c mice delayed hypersensitivity to mycobacterial antigens was induced by the sensitization of the animals with live BCG or killed Mycobacterium bovis or M. avium in incomplete Freund's adjuvant. In the study of the dynamics of the development of skin reactivity to tuberculin some advantages of the sensitization of guinea pigs with live mycobacteria were revealed, while after the revaccination of the animals no development of secondary cell-mediated immune response was observed. The immunization of guinea pigs with atypical mycobacteria prior to their sensitization with BCG was found to lead to the development of higher skin reactivity to allergen prepared from atypical mycobacteria than skin reactivity to tuberculin.

Animals↗
PubMed1990

[Isolation of antibodies against species-specific M. bovis antigens].

Tuberculin PPD antibodies were isolated from hyperimmune rabbit serum by affinity chromatography. They were crossover depleted for elimination of antibodies specific to common (with atypical mycobacteria) antigens. Antibodies to M. bovis species-specific antigens may be used for preparing an immunosorbent and affinity chromatographically isolating the antigens from tuberculin PPD.

Animals↗
PubMed1990

[The role of T-lymphocytes in the development of a humoral immune response to the corpuscular antigen of Staphylococcus aureus].

The dependence of humoral immune response and the formation of immunological memory to corpuscular staphylococcal antigen (CSA) on the T-system of immunity was studied in experiments on B-mice and on mice with the congenital absence of the thymus (nude). Primary and secondary immune response to CSA in athymic mice was found to be considerably less than in normal animals. After the repeated immunization of genetically athymic mice the pronounced secondary reaction of the formation of antibodies to CSA was observed. As shown in this investigation carried out with the use of adoptive transfer techniques, the induction of memory B-cells to CSA may occur in animals with congenital or experimentally induced T-immunodeficiency. The conclusion was made on the T-dependence of humoral immune response to CSA, the formation of immunological memory to this antigen being relatively T-independent.

Animals↗
PubMed1989

[The role of the bone marrow in forming an immunological memory to Staphylococcus].

The dynamic study of antibody formation in the bone marrow tissue of mice immunized with corpuscular staphylococcal antigen was made. A single intravenous injection of this antigen to the animals produced no essential changes in the number of antibody-forming cells in the bone marrow, their repeated immunization led to the involvement of the bone marrow in the anamnetic reaction, which was manifested by the accumulation of a great number of plasmocytes synthesizing antibodies to staphylococci in bone marrow tissue. The relative contribution of the bone marrow to systemic antibody formation essentially increased at later stages of secondary immune response. The possible biological significance of the regularities and the practical importance of the data revealed in this investigation are discussed.

Animals↗
PubMed1986

[Mechanism of formation of antibody production stimulating ability of bone marrow cells of mice immunized with staphylococci].

The mechanism of the increase in immune response to particular staphylococcal antigen was studied in CBA and BALB/c mice injected by primed bone marrow cells (BMC). It was found that immunostimulatory effect of immune BMC is not mediated by macrophages or T cells, but is associated with staphylococcus-specific B memory cells present in the pool of primed BMC. Splenectomy performed in donor animals prior to immunization did not abolish the induction of stimulating BMC activity. It was concluded that primed B lymphocyte migration from spleen into bone marrow is not obligatory for the induction of staphylococcus-specific immunological memory in the bone marrow.

Animals↗
PubMed1986

[Immunologic memory for Staphylococcus studied by adoptive transfer].

The basic regularities of the formation and realization of immunological memory to staphylococcal corpuscular antigen were studied in adoptive transfer experiments on CBA mice. The capacity of spleen cells for generating anamnestic response to staphylococci in the body of irradiated syngeneic recipients appeared on day 3 after the immunization of donors. The formation of immunological memory to staphylococci in mice was shown to be directly related to the dose of the antigen. The study also revealed that intact splenocytes did not suppress the realization of immunological memory to staphylococci in the system of adoptive transfer. The conclusion of the absence of the "isogeneic barrier" for memory cells specific to staphylococcal corpuscular antigen was made.

Animals↗
PubMed1985

[Mechanism of the intensification of the immune response to Staphylococcus in mice after the transplantation of primed donor splenocytes].

Experiments on CBA, C57Bl/6 mice and (CBA X X C57Bl/6)F1 hybrids were made to study the mechanism of stimulation of the immune response to staphylococci after injection of primed splenocytes. The stimulating action of immune splenocytes was reversed after their in-vitro treatment with anti-immunoglobulin serum and complement. The stimulant effect was also seen in a semi-allogeneic system (adoptive transfer of CBA mice immune cells to (CBA X C57Bl/6)F1 recipients). Preincubation of splenocytes with CBA-anti-C57Bl/6-serum and complement prior to demonstration of antibody-forming cells did not influence their number in the spleen of hybrid recipients injected with immune cells carrying parent genotype but decreased this indicator of the immune response in control mice. It is concluded that stimulation of the immune response to staphylococci after transplantation of primed splenocytes is due to the anamnestic response of donor's cells repeatedly stimulated by antigen in the recipient's host.

Animals↗
PubMed1985

[Immune response and the formation of immunologic memory to Staphylococcus in mice of different genotypes].

The time course of changes in the number of antibody-forming cells in the spleen in the primary and secondary immune response to staphylococcal corpuscular antigen was studied in experiments on mice. C3H mice were found to be highly responsive, while A/Sn mice showed low response, the opposite character of immune responsiveness to this antigen in the animals of the above-mentioned genotypes increasing after the second immunization. C57BL/6, CBA, DBA/2, BALB/c and (CBA X C57BL/6) F1 mice showed comparatively moderate antibody formation in response to staphylococci. The formation of immunological memory to this antigen depended on the genotype of mice and was determined by the intensity of antigenic action: high priming doses of staphylococci proved to be most effective; low doses of the antigen were not effective or produced only short-term immunological memory to staphylococci in mice.

Animals↗
PubMedDate not supplied

[Chromatographic analysis and immunobiologic properties of tuberculins].

Immunobiologic activities of tuberculin preparations and their components have been comparatively studied using gel filtration and high-performance liquid chromatography (HPLC) techniques. It is shown that high-molecular weight fraction of protein-purified derivate tuberculin (PPD) had higher activity as compared to nonfractionated preparation in skin tests on guinea pigs sensitized with Mycobacterium bovis BCG as well as in enzyme-linked immunosorbent assay with affinity purified rabbit antibodies against PPD. Using the preparative HPLC-technique we failed to isolate a component of PPD having greater tuberculin test potency than nonfractionated preparation.

Animals↗
PubMedDate not supplied

[Morpho-functional interrelations in the development of immunologic memory].

Data available in literature on immunomorphological aspects of the immunological memory formation are summarized. Features of B memory cells and mechanisms of their activation are analyzed. Relations between antigen localization in the lymphoid follicles, the formation of virgin centres there and induction of B memory cells and organs of the immune system in the immunological memory are discussed.

Animals↗
PubMedDate not supplied

[Patterns of the forming of an immunologic memory to staphylococcal corpuscular antigen].

The experiments carried out on inbred mice have revealed that the level of the immunological memory to staphylococci depends on the intensity of the antigenic stimulation; high priming dose of antigen proving to be the most effective one. The opposite character of immune responsiveness observed during primary antibody response to particulate staphylococcal antigen in C3H and A/Sn mice increased after the second immunization. It is established that immunological memory to staphylococci may be induced in genetically athymic mice. Many antibody-forming cells are found in the bone marrow of the secondary immunized mice. This phenomenon may be due to the repopulation of the bone marrow tissue by recirculating memory cells.

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