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K Pardue

Publications and source records attributed to K Pardue.

3 recordsLinked to original sources
PubMed1994

Structural dynamics in an electron-transfer complex.

The dynamic behaviour of the complex of horse cytochrome c with cytochrome c peroxidase, an electron-transfer complex, was studied in solution by a hydrogen exchange labelling method together with two-dimensional NMR analysis. Although cytochrome c hydrogens in the expected binding region exhibit slowed exchange, the measured slowing factors are very small, indicating that hydrogen-exchange occurs with little hindrance from within the binding interface. The complex in solution must therefore be highly mobile rather than rigidly defined, as implied by the crystalline complex. This result is in conflict with the concept that biological electron transfer occurs by way of predetermined covalent pathways.

Amino Acid Sequence↗
PubMed1993

Quantitative determination of non-ionic surfactants in protein samples, using ion-exchange guard columns.

Quantitative determination of Triton X-100 in buffered, organic modifier-containing solutions was performed by use of strong cation- and anion-exchange HPLC guard columns. Recovery was complete for Triton X-100 concentrations of 0.1%, 0.05%, 0.25% and 0.0125% (wt/wt) in 50 mM potassium phosphate buffer (pH 5.0 +/- 0.05, pH 4.0 +/- 0.05, pH 3.0 +/- 0.05 and pH 2.0 +/- 0.05) modified with 30% isopropanol. This procedure can be applied to recovery of other nonionic surfactant systems.

Animals↗