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Biomedical subjects

K Pasternak

Publications and source records attributed to K Pasternak.

13 recordsLinked to original sources
PubMed1999

[Magnesium and zinc levels in blood serum and cerebrospinal fluid in children with febrile convulsions].

Magnesium and zinc levels in blood serum and cerebrospinal fluid were estimated in 18 children aged 8 months-5 years with febrile convulsions. Control group consisted of 15 apparently healthy children in the same age. On the ground of ethical reasons no control values for cerebrospinal fluid were evaluated. The mean serum concentration of magnesium and zinc was significantly lower in the sick children (p < 0.001 and p < 0.05 resp.). The magnesium levels in cerebrospinal fluid were evidently lower than those quoted in the literature and the mean concentration of zinc was higher in comparison with literature data. The presumable pathogenetic role of disturbed magnesium and zinc metabolism in febrile convulsions is discussed.

Child, Preschool↗
PubMed1999

Magnesium in stomach cancer.

Experiments were conducted in order to determine magnesium concentrations in stomach cancer tissue. As a control, non-cancerous stomach tissue of the same patients was used. Mg concentration in blood serum was also tested in all patients undergoing surgical operation. It has been determined that Mg concentration was higher in cancerous stomach tissue than in control tissue. Average Mg concentration in the blood serum of patients with stomach cancer fluctuates near the lower limit of normal. An analysis of the relationship between Mg concentration and the degree of clinical advancement of cancer has shown that Mg concentration in cancerous stomach tissue increases with the clinical stage. The results obtained were statistically significant. They show that there is a relationship between Mg concentration and the development of stomach cancer.

Gastric Mucosa↗
PubMed1999

Tissue concentrations of magnesium in rats receiving various dosages of ethanol.

Ethanol exerts various influences on living organisms. The experiments were conducted in order to determine the influence of ethanol on changes of magnesium concentration in various tissues. The experiment was conducted on white Wistar rats of both sexes. The animals were divided into groups of ten rats each. They received ethanol in their drinking water for 6 weeks. The first group received 5 per cent ethanol, the second received 20 per cent ethanol, the third was the control group and received water. All animals were fed with the same laboratory feed and watered ad libitum. After the duration of the experiment the animals were sacrificed using ketamine. The brain, liver, heart, lung, kidney, spleen, femoral muscle and blood serum were then harvested for further testing. The magnesium concentration was tested in these tissues by using atomic absorption spectrophotometry (AAS). Ethanol in feed causes a shift in the tissue concentration of magnesium. The range of these concentration differences is determined by the dosages as well as by the type of tissue.

Administration, Oral↗
PubMed1999

The influence of ethanol and magnesium on aminoacyl-tRNA synthetases activity in rat's liver.

Ethanol exerts various influences on living organisms. Experiments were conducted in order to determine the influence of ethanol and magnesium on the aminoacyl-tRNA synthetases (aaRS) activity in rat's liver. The experiment was conducted on white Wistar rats of both sexes. The animals were divided into groups of 10 rats each. They received ethanol in their drinking water for 6 weeks. The first group received 5 per cent ethanol, the second received 20 per cent ethanol, the third received 20 per cent ethanol and 200 ppm magnesium, the fourth was the control and received water. After the duration of the experiments the animals were sacrificed using ketamine and the livers were harvested for further testing. Aminoacyl-tRNA synthetases were obtained by salting out with ammonium sulphate. The aminoacyl-tRNA synthetases activity were tested in vitro by using marked amino acids. Ethanol influences aminoacyl-tRNA synthetases activity. The influence of ethanol on the activity of aaRS depended on the size of the dose of ethanol taken by examined animals. At the same time it was revealed that magnesium supplementation limits the negative effect of high ethanol doses.

Administration, Oral↗
PubMed1998

The effect of a single dose of morphine and ethanol on magnesium level in blood serum and tissues in mice.

The effect of a single dose of morphine (50 mg/kg) and ethanol (2 g/kg) on total magnesium content in blood serum, brain, heart, lung, kidney, liver, femoral muscle and spleen in mice was studied. Significant decrease of magnesium serum concentration was observed after morphine and ethanol administration but not after both drugs given simultaneously. Morphine caused the evident decrease of magnesium content in brain, lung, kidney and muscle, while it was elevated in heart and spleen and unchanged in liver. Ethanol produced significant decrease of magnesium content in heart, lung and kidney and it's increase in liver and spleen. Concomitant administration of both drugs was connected with the diminished amount of magnesium in heart, lung, kidney and muscle and led to the rising of magnesium content in spleen. It is concluded that even a single dose of investigated drugs is sufficient to produce promptly some risk of alterations in magnesium homeostasis.

Animals↗
PubMed1997

The effectiveness of the bonding of amino acid by tRNA of livers of rats experimentally exposed to cadmium and barium.

The process of amino acid activation takes place in the presence of specific aminoacyl-tRNA synthetases. These enzymes, together with ATP, catalyse the bonding of tRNA and amino acids (5). The effectiveness of binding amino acid by tRNA has a direct influence on the intensity of protein biosynthesis. This effectiveness varies in various metabolic states of the cell (16). Heavy metals influence cellular metabolism. They usually reduce enzyme activity, block chemical reactions, bind with proteins, interact with other elements and even break certain bonds (1, 8, 9, 11, 14). Experiments in the present work were conducted in order to determine changes in the effectiveness of amino acid binding by tRNA of the livers of rats receiving heavy metals such as cadmium and barium in their feed.

Adenosine Triphosphate↗
PubMed1997

[Activity of isocitrate dehydrogenase in breast cancer].

The activity of isocitrate dehydrogenase (ICDH) was studied in the tissue of breast cancer and in the blood serum of the women suffering from this neoplasm. The comparative material was the physiological tissue of mammae and blood serum of the healthy women. The activity of ICDH in neoplasm tissue was more higher than in physiological tissue. In the blood serum of ill patients activity of this enzyme was higher than in control serums too.

Breast Neoplasms↗
PubMed1995

Mitochondrial tRNA in hyperthyroidism.

The mitochondrial tRNA were prepared from liver and brain tissues of thyroxinized and control rabbits. The presence of tRNA for twenty amino acids both in liver and brain mitochondria was revealed. The quantity of radioactive amino acids bound to the mitochondrial tRNA was higher in hyperthyreosis than in control animals but considerable differences between the brain and liver tissues were observed.

Animals↗
PubMed1994

Activity of aminoacyl-tRNA synthetases in experimental hyperthyroidism in muscle tissues of the rabbit.

In cardial and femoral muscles of rabbits specific activities of aminoacyl-tRNA synthetases for twenty amino acids were generally similar, namely the activities towards amino acids and their amides, leucine, isoleucine, histidine, tyrosine, proline and serine were considerably lower than towards the remaining amino acids. Specific activities of most aminoacyl-tRNA synthetases were higher in hyperthyroidism than in euthyreosis, and were higher in femoral muscle than in heart. The response to thyroxine treatment of individual aminoacyl-tRNA synthetases in both kinds of muscles varied with respect to most of the amino acids.

Amino Acyl-tRNA Synthetases↗
PubMed1993

[Tissue levels of some elements in hyperthyreosis in rabbits].

The aim of the present study was to estimate the concentration of Cu, Zn, Mg, Ca in the following tissues: brain, heart, lung, liver, kidney and femoral muscles in conditions of experimentally induced hyperthyreosis. In general, in state of hyperthyreosis the concentration of all elements was considerably higher compared to euthyreosis. However, there are a few exceptions. Liver and heart tissue possessed higher concentration of Zn and Cu and kidney of Cu in euthyreosis.

Animals↗