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Biomedical subjects

K Pedersen

Publications and source records attributed to K Pedersen.

At least 163 records · Page 9Linked to original sources
PubMed1979

Synthesis and processing of maize storage proteins in Xenopus laevis oocytes.

Xenopus oocytes injected with zein mRNAs efficiently synthesized maize storage proteins for prolonged periods. Under optimal conditions, zein was synthesized at 3 ng/hr and represented approximately 10% of the total protein synthesized in the oocyte. The mRNA from the normal maize inbred line directed synthesis of all the major zein components; however, products of mRNA from the opaque-2 mutant did not contain the largest zein component. Zein proteins synthesized in the oocyte were 2000 daltons smaller than proteins synthesized by cell-free translation of mRNAs in the wheat germ and reticulocyte systems. This result, which suggested that the oocyte processed prezein polypeptides into native zein proteins, was confirmed by amino-terminal sequence analysis of zein proteins from the oocytes. Cyanogen bromide cleavage of translation products from oocytes and the wheat germ system confirmed the existence of several proteins within each of the major zein components. However, we were unable to detect the presence of signal sequences on zein peptides by this technique.

Journal Article↗
PubMed1978

Grand mal-provoked hyperuricemia.

A significant increase in S-urate was found postictally in 17 patients with two or more grand mal seizures within 24 h. In six patients S-urate was above the level at which hyperuricemic renal failure may develop. Impaired renal function was observed in two patients who had extremely high S-urates. It is proposed that prophylactic procedures against hyperuricemic renal failure should be carried out in all patients with repetitive convulsions.

Acute Kidney Injury↗
PubMed1977

Gonial angle distortion in lateral head films: a methodologic study.

The distortion of the gonial angle in lateral roentgenographic head films was studied in skull material. The left and the right gonial angles were determined craniometrically and roentgenocephalometrically, and the observations were compared. Mean difference of 8.48 degrees and 5.15 degrees between the roentgenocephalometric and the craniometric registrations were found for the side closer to the film and the side farther away from it, respectively. For the intermediate gonial angle, the difference was 6.65 degrees. The differences were all statistically significant. It was demonstrated that they were mainly attributable to a systematic method error in the roentgenocephalometric method, involving a magnification of the gonial angles. The magnification was significantly larger on the side closer to the film than on the side farther away from it. A pronounced individual variation in gonial angle distortion was observed. For intermediate gonial angle it ranged between magnifications of 1.17 degrees and 17.54 degrees. The distortion is closely associated with the form of the mandible. It is largest in mandibles with a pronounced divergence of the two halves of the corpus and a pronounced upward convergence of the rami. It is smallest in mandibles with a modest divergence of the two halves of the corpus and a pronounced upward divergence of the rami. By some very special combinations of corpus divergence and ramus inclination, a correct reproduction may be obtained. No such cases were found in the sample studied, but there can hardly be any doubt that they exist. There is an association between corpus divergence and ramus inclination. Maximum magnifying effect of the forward-extending leg tends to coincide with the maximum magnifying effect of the upward-extending leg. Conversely, minimum magnifying effect of the forward-extending leg tends to coincide with a dimishing effect of the upward-extending leg. This is the clue to an understanding of the great individual variation in gonial angle distortion. Generally speaking, lateral head films do not permit reliable registrations of the gonial angle. At present no method is available for an exact assessment of the distortion in head films of the single living person.

Adult↗
PubMed1976

A multidimensional study of personality traits ad modum Sjöbring.

Three groups of epileptic out-patients (juvenile myoclonic epilepsy, psychomotor epilepsy, and cryptogenic grand mal) were studied. The groups were matched as closely as possible with each other with regard to sex, age, and treatment. Out-patients suffering from Menière's disease served as a control group; these patients were treated with placebo and matched with the epilepsy groups with regard to sex, duration of disease and social level. The Marke-Nyman inventory was used as a quantitative assessment of personality traits. This inventory is an operative definition of Sjöbring's neurophysiological model of personality, including the three dimensions: validity, stability and solidity. Our results showed that epileptic patients irrespective of the type of seizures were substable. Low validity was found in patients with juvenile myoclonic epilepsy and in patients with psychomotor epilepsy with temporal EEG focus. In those latter epilepsy groups a tendency to subsolidity was also observed. In Sjöbring's frame of reference these substable patients of low validity have a psychological vulnerability since they are unable to overcome the small concrete adversities of life. They adhere to problems, and being unable to solve them they tend to react in a mood of discontent or of maladjustment. In the usual psychiatric frame of reference substable patients of low validity are classified as psychoastenic patients with emotional instability.

Adolescent↗
PubMed1974

Separation and partial characterization of multiple ribonucleic Acid polymerases from soybean hypocotyl.

Two major peaks of RNA polymerase activity have been routinely separated by diethylaminoethyl cellulose chromatography following solubilization from soybean (Glycine max L. var. Wayne) chromatin. The relative amounts of these two peaks depend upon the manner in which the chromatin is purified. Pelleting the chromatin through dense sucrose solutions results in not only a loss of total solubilized RNA polymerase activity but also a selective loss of the alpha-amanitin-sensitive form of the enzyme. Peak I elutes from a diethylaminoethyl cellulose column at a KCl concentration of approximately 0.27 m, is insensitive to alpha-amanitin and rifamycin, and has Mg(2+) + Mn(2+) optima of 5 mm and 1.25 mm, respectively. The enzyme is inhibited by KCl concentrations of about 0.03 m or greater. Peak II elutes from the column at a KCl concentration of approximately 0.35 m, is sensitive to alpha-amanitin, insensitive to rifamycin, and has Mg(2+) + Mn(2+) optima of 2 mm and 1.0 mm, respectively. Activity is inhibited by KCl concentrations of about 0.06 m or greater. Both enzymes prefer denatured calf thymus DNA, but peak II exhibits a stronger preference.

Journal Article↗