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Biomedical subjects

K Pirlet

Publications and source records attributed to K Pirlet.

At least 19 recordsLinked to original sources

[Nature's healing power--natural selection in protein turnover].

For millennia, medical practitioners, healthy people and the sick have known that there is an inner force that keeps us sound and also cures us. However, neither medicine nor natural sciences have been able to define this curative power. It can not be captured by experiments or assigned with dimensions or numbers. The answer can be found in the quintessential process of all living systems, the interaction of genes and proteins in every single cell. Genes encode proteins; proteins are the ubiquitous instruments of life. Protein molecules undergo rapid turnover: their median lifetime is about 2 days, and in every second so many proteins are synthesized in each of us that their number equals the number of seconds that have passed since the big bang 15 billion years ago. The biochemical dogma that says that after their synthesis proteins are degraded at random - blindly, quasi - is wrong. The experimental results have not been interpreted correctly. In the first instance, proteins are stable and functional, since they have to fulfill numerous tasks. Only aged, severely damaged, non-functioning proteins are discarded. Protein turnover is subject to selection, not to chance. By selecting fresh, functional proteins, the quality of cellular proteins is kept at a high level. The principle of selection in protein turnover is the crucial component in the scientific groundwork of naturopathy therapeutics. With natural influencies and measures (e.g. food processing, intermediate metabolism, exercise, light, warmth, coldness) the steady process of regeneration is accelerated - in contrast to medical therapy, which restricts and blocks protein activities. Natural healing power and self-organization energy are basic phenomena of a therapeutically used physiology. Naturopathy is natural science.

Biological Evolution↗

Maintaining life and health by natural selection of protein molecules.

A concept for a life and health-preserving principle is presented, with reference to evolutionary, medical, and biochemical observations. Life comprises two basic phenomena: it unfolds over longer periods at the population level, and is sustained for the duration of individual life spans. The evolution of life within populations by means of natural selection of individuals is central to Darwin's theory of evolution. An important component of maintaining individual life is proposed here to be the natural selection of molecular components-the proteins, a process of preferred removal of denatured and old, synonymous with the selection of younger, functional molecules. The proteins of the cell are committed to fulfilling all the tasks programmed by the genome while continuously maintaining all appropriate cellular functions, including protecting the DNA. Physiological and environmental influences accelerate the breakdown of aged protein molecules, driving this renewal process so that the cell can maintain its protein stock at high-performance levels. The principle of selection makes the incredible dynamics of continual protein turnover, and hence not only the preservation of life, but the maintenance of health in individual beings, comprehensible. Arguments are presented to counter the hypothesis that protein breakdown is a stochastic, random process governed by first-order kinetics.

Animals↗

Antibodies to dsDNA: failure to detect antigenic determinants specific for superhelical B-DNA.

IgG antibodies of SLE sera were tested for their reactivity with SV40 DNA in superhelical, open circular and linear conformations. In binding and competition studies with 12 IgG dsDNA positive sera we could not demonstrate antibodies reacting with antigenic sites specific for superhelical structures of DNA, nor did we find Z-DNA antibodies reacting with superhelical SV40 DNA.

Antibodies, Antinuclear↗

Antibodies to histones of the IgG and IgM class in systemic lupus erythematosus.

Antibodies to histones were measured by an ELISA. Histone antibodies of the IgM class could be detected in other diseases than SLE, as well as in healthy persons. However, IgG histone antibodies proved to be specific for SLE and could be found in 21% of the SLE patients. These antibodies were highly associated with dsDNA antibodies of the IgG class. We could not find any correlation between IgG histone antibodies and clinical symptoms of SLE. IgG histone antibodies mostly reacted equally with all tested histone fractions.

Antibodies, Antinuclear↗

[Quantitative changes in peripheral T-lymphocytes during hyperthermia (author's transl)].

A uniform, highly significant fall in T-lymphocytes in the peripheral blood, without any change in the absolute lymphocyte count occurred in 6 healthy test subjects in a standardized hyperthermal bath with a mean rise in the rectal body temperature of 1.35 degrees C. A redistribution from the blood into the tissue structures of the rapidly recirculating T-lymphocytes in the body is assumed. The clinical significance of this redistribution cannot yet be stated. With increased recirculation of immunoreactive T-lymphocytes an increased contact with antigenic material in the tissues traversed would be possible. This could be the basis of the "immunostimulation" during hyperthermia assumed by some authors.

Adult↗

Enzyme immunoassay for IgG and IgM antibodies against dsDNA and ssDNA.

Two enzyme-linked immunosorbent assays (ELISAs) for the detection of DNA antibodies are presented, one using lambda-phage DNA for the detection of dsDNA antibodies, the other using denatured calf thymus DNA for ssDNA antibodies. It was shown that only dsDNA antibodies of the IgG class were specific for SLE. IgM-dsDNA antibodies or ssDNA antibodies were not SLE-specific. Of 62 SLE patients 58% were positive for IgG-dsDNA antibodies. When only those patients with active disease were considered, 88% were positive. There was a positive correlation between the levels of IgG-dsDNA antibodies and the incidence of nephritis. When the ELISA for IgG-dsDNA antibodies was compared with the filter assay, the ELISA showed a considerably higher sensitivity.

Arthritis, Rheumatoid↗