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Biomedical subjects

K R Perry

Publications and source records attributed to K R Perry.

23 records · Page 2Linked to original sources
PubMed1989

Diagnosis of hepatitis A and B by testing saliva.

The use of salivary samples to diagnose acute viral hepatitis was investigated. Tests for IgM antibody to hepatitis A virus (anti-HAV) on 29 acute-phase samples from serologically confirmed cases of hepatitis A were strongly reactive. Follow-up samples indicated that IgM anti-HAV persisted at moderate levels for 2-4 months and was not usually detectable thereafter. The ratio of IgM to IgG anti-HAV (RIA index) correlated closely with the interval from onset of infection. Significant levels of IgM anti-HAV were not detected in the saliva of 103 IgG anti-HAV positive and 102 IgG anti-HAV negative individuals nor of 4 individuals with hepatitis B. Similarly, IgM anti-HBc was present in the saliva of acute cases of hepatitis B, but not in the saliva of 25 IgG anti-HBc positive and 85 IgG anti-HBc negative individuals, nor of 24 individuals with recent hepatitis A. It is concluded that saliva is a convenient and satisfactory alternative to serum for the diagnosis of hepatitis A infection.

Disease Outbreaks↗
PubMed1989

Investigation of an outbreak of hepatitis A simplified by salivary antibody testing.

In March 1988 a general practitioner notified two cases of hepatitis A in a private boarding school. Epidemiological investigation, including testing for salivary antibodies revealed a further five cases and established immunity to, and recent infection with, hepatitis A virus (HAV). The pattern of the outbreak was described. A number of practices which would encourage cross-infection were corrected. Normal human immunoglobulin was given to contacts. Repeat salivary testing 10 weeks later revealed that two more boys had become reactive for anti-HAV, though at a low titre. These may have been serological responses to HAV infection modified by the passive immunization.

Adolescent↗
PubMed1988

Rational programme for screening travellers for antibodies to hepatitis A virus.

A blood test and a saliva test for antibody to hepatitis A virus (anti HAV) were offered to British travellers seeking human normal immunoglobulin (HNIG) prophylaxis. The specimens were tested by an IgG capture and a competitive radioimmunoassay (GACRIA, COMPRIA). By GACRIA 211 subjects were anti-HAV positive and 358 anti-HAV negative on both serum and saliva. 10 other seropositive subjects had weakly positive saliva reactions. There were three discrepant results. For the population investigated HNIG use could be minimised at no extra cost by first testing the saliva of those greater than 40 years old, frequent or long-stay travellers, those born in HAV endemic areas, and those with a history of jaundice. Of the 51% of travellers tested for these reasons 20% were anti-HAV positive. They made up 76% of all those with antibody.

Antibodies, Viral↗
PubMed1987

Sensitive assays for viral antibodies in saliva: an alternative to tests on serum.

Paired serum and saliva specimens were tested by conventional assays and by IgG-capture radioimmunoassays (GACRIA) and enzyme-linked immunosorbent assays (GACELISA) for antibody to hepatitis A virus (HAV, 100 pairs), human immunodeficiency virus (HIV, 53), hepatitis B virus core (HBc, 62), and rubella virus (30). Conventional assays failed to detect viral antibodies in the saliva of 93 of 119 seropositive subjects. However, GACRIA detected the antibodies in both serum and saliva of all subjects seropositive by conventional tests, except 2 saliva specimens false-negative for anti-HBc and 1 false-negative for anti-rubella-virus. For anti-HIV and anti-HBc serum and saliva GACRIA reactivities did not differ significantly, but anti-HAV and anti-rubella-virus GACRIA reactivities were stronger in serum than saliva. GACRIA and GACELISA results on saliva for the four antibodies correlated closely; for anti-HAV and anti-HIV GACELISA and GACRIA were equally accurate. For both saliva and serum, GACRIA was superior to an IgA-capture assay in detecting anti-HAV and anti-HIV.

Antibodies, Viral↗