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Biomedical subjects

K R Weaver

Publications and source records attributed to K R Weaver.

6 recordsLinked to original sources
PubMed1998

Family predictors of suicidal symptoms in young adolescents.

A 1-year longitudinal study tested the model that the relation between maternal depression and adolescent suicidal symptoms is mediated by family functioning. Participants were 240 children (mean age = 11.86 years) and their mothers; 77% of the mothers had a history of a mood disorder and the remaining 23% were lifetime-free of psychopathology. An adolescent suicide index was created based on suicide items from the child and parent versions of the Children's Depression Inventory, Child Behavior Checklist and Children's Depression Rating Scale, administered at both Time 1 and 2. Family functioning was assessed with the Family Relationship Index completed by mothers and children at Time 1. Results indicated that the relation between maternal depression and adolescent suicide symptoms at Time 2 was mediated by perceived family functioning, controlling for suicide symptoms at Time 1.

Adolescent↗
PubMed1996

Automated polymerase chain reaction product sample preparation for capillary electrophoresis analysis.

The analysis of crude polymerase chain reaction (PCR) products by capillary electrophoresis (CE) is often compromised due to the presence of a high concentration of salt. Salt interferes with the electrokinetic injection and induces localized heating within the column; hence, PCR products must be desalted or cleaned-up prior to CE analysis. A variety of commercial clean-up systems are available that have been traditionally used to prepare PCR products for cloning, sequencing and digestion with restriction enzymes. These systems were tested for their effectiveness in preparing PCR products for CE analysis and were evaluated based on CE resolution, salt removal, DNA recovery, processing time and cost. One particularly effective clean-up system, membrane dialysis, was automated using a robotic workstation.

Automation↗
PubMed1996

Characterization of mitochondrial DNA using low-stringency single specific primer amplification analyzed by laser induced fluorescence--capillary electrophoresis.

Polymerase chain reaction (PCR)-based DNA typing is routinely used in forensics for identity testing. Those assays that distinguish single nucleotide polymorphisms (SNPs) require other biochemical reactions in addition to PCR to identify the sequence polymorphisms. Low-stringency sequence-specific PCR (LSSP-PCR) is an example of a recent method that does not require additional biochemical treatments. The analysis of LSSP-PCR by capillary electrophoresis (CE) to discriminate the highly polymorphic mitochondrial DNA (mtDNA) D-loop region is described. The DNA from five individuals were amplified (first step) using sequence-specific primers to produce 1021 bp fragments containing the D-loop region. Each fragment was isolated by electroelution using CE and UV detection, and subjected to a second amplification (second step) using a single primer annealed under low stringency conditions. This generated a range or profile of PCR products for each sample, which were resolved and analyzed by CE with the intercalator TOTO-1 and laser-induced fluorescence (LIF) detection. The LSSP-PCR profiles were unique for each individual, indicating that this technique may be applicable for forensic identity testing.

DNA Primers↗
PubMed1996

The evaluation of fast purification methods for preparing polymerase chain reaction (PCR) products for capillary electrophoresis analysis.

The analysis of crude polymerase chain reaction (PCR) products by capillary electrophoresis (CE) is often compromised by the presence of a high concentration of salt. Salt interferes with the electrokinetic injection and induces localized heating within the column, hence, PCR products must be desalted or cleaned-up prior to CE analysis. A variety of commercial clean-up systems are available that have been traditionally used to prepare PCR products for cloning, sequencing, and digestion with restriction enzymes. These systems were tested for their effectiveness in preparing PCR products for CE analysis and were evaluated based on CE resolution, salt removal, DNA recovery, processing time, and cost.

DNA↗
PubMed1995

Automated DNA purification and amplification from blood-stained cards using a robotic workstation.

A new method for the purification of DNA on blood-stained cards was developed. This method was implemented into a high-throughput automated system using a Biomek 1000 robotic workstation. In addition, the processes of DNA purification and amplification were coupled into a completely automated and uninterrupted prototype system, and the resultant PCR products generated by this system were subjected to automated desalting for capillary electrophoresis analysis.

Autoanalysis↗