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Biomedical subjects

K Rehn

Publications and source records attributed to K Rehn.

At least 19 recordsLinked to original sources
PubMed2002

[Development of a CAN (controller area network) bus for modules of a perfused cell culture system].

For computer-aided data acquisition and automated running of experiments in cell cultivation reactors networking of all sensoric and actoric devices is required. A low cost and high performance solution to this demand can be found by using the widely established CAN (Controller Area Network) bus. If standard PCs running MS Windows are used to control this network special measures have to be taken to prevent loss of data caused by differences in the computing power of the PC on one hand and microcontroller-based devices on the other.

Cells, Cultured↗
PubMed1991

Transdermal oestrogen replacement therapy in a Finnish population.

In an open, multicentre study, transdermal administration of oestradiol (E2) by means of skin patches was investigated in a Finnish patient population suffering from typical post-menopausal symptoms. A total of 249 women applied a patch twice weekly for 6 months. Whereas 85% of the subjects were experiencing hot flushes and 83.5% sweating before therapy, only 5.7% and 11.8%, respectively, reported these symptoms at the end of the trial. Furthermore, 97.6%, 95.7% and 94.8% of the subjects reported that depression, headache and sleep disturbances, respectively, had disappeared during therapy. Skin irritation occurred in 18.2% of these predominantly fair-skinned women. Frequent sauna bathing did not interfere with the patch therapy. General acceptance of the treatment was excellent, 84.8% of the patients completing the treatment, of whom 78% were willing to continue the treatment after the trial. These results show that transdermal administration of E2 is effective in relieving post-menopausal symptoms. Local tolerability was good and the majority of the patients considered the transdermal treatment to be superior to their previous oral replacement therapy.

Administration, Cutaneous↗
PubMed1975

[Proceedings: Hematopoiesis in chronic pyelonephritis. Quantitative-morphological study].

Bone marrow biopsies from the iliac crest in 56 patients with chronic pyelonephritis were evaluated quantitively and correlated with renal function and peripheral blood values (n = 100). In proportion to increasing serum creatinine, mild marrow hypoplasia develops which mainly, and to a statistically significant extent, affects the percentage and amount of erythropoietic cells and megakaryocytes. The simultaneous reduction of erythrocytes and reticulocytes in peripheral blood is more pronounced and only slightly correlated to the marrow cells. There is no correlation between the number of peripheral granulocytes or the decrease in lymphocytes concomitant with uremia, and their respective precursors in the marrow.

Bone Marrow Examination↗
PubMed1975

[High titer cold agglutinins with anti-pr specificity after rubella infection (author's transl)].

High titer cold agglutinins (CA) after rubella infection are reported. When the rubella exanthema disappeared the clinical aspect of a cold agglutinin disease was observed. Three weeks after the appearance of the cutaneous eruption the CA titer reached a maximum of 1/8000, to then continuously fall off to normal values within 20 weeks. Double diffusion tests showed that the isolated CA were IgM proteins that possess only chi-type light chains. In spite of normal protein- and immunoelectrophoresis patterns obtained with whole serum samples, the isolated CA showed restricted electrophoretic mobility and a deformation of the precipitate typical for monoclonal immunoglobulins. In contrast to the common anti-I specificity of IgM CA, the IgM CA described showed anti-Pr specificity. Possible interrelations between CA specificities and types of germs inducing reactive cold agglutination are discussed.

Adult↗
PubMed1975

[A simplified macrophage migration inhibition test to measure T-cell derived immunity in man].

A macrophage migration inhibition test using heterologous cells (DMMTH) is performed in an agarose-droplet technique. A suspension consisting of peritoneal exsudate cells from Guinea pig and human lymphocytes, mixed with 0,2 percent agarose is dispensed in droplets with a dispenser into culture chambers used for the leukocyte migration inhibition test. Macrophages migrate out of the gelified droplets. Migration areas can be measured by planimetry. Migration areas are exact to 8.6 percent. A significant migration inhibition, caused by migration inhibitory factors is observed after the reaction of the specific antigen with sensitized lymphocytes in the agarose droplet. The sensitivity of the test has been demonstrated in Mantoux positive persons.

Cell Migration Inhibition↗
PubMed1973

Cell envelope and shape of Escherichia coli K12.

Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining material is lipopolysaccharide. Sodium dodecyl sulfate-gel electrophoresis reveals 4-5 different bands corresponding to molecular weights between 10,000 and 40,000. Treatment of ghosts with Pronase reduces this number to 3, and the rod shape still is not lost. Results of treatment of ghosts with a crude extract from Dictyostelium discoideum have supplied tentative evidence that at least one of these proteins is involved in the maintenance of rod shape. It does not appear too unlikely that these polypeptide chains are the final products of the genetic information specifying cellular shape.

Bacterial Proteins↗
PubMed1973

Model for the structure of the shape-maintaining layer of the Escherichia coli cell envelope.

The surface area per repeating murein unit (i.e. per molecule of diaminopimelate) has been determined for the cell envelopes of the Escherichia coli strains K-12 and W. This area was constantly found to be 1.3 nm(2). Using this value and other previously determined properties of E. coli murein, a three-dimensional model of murein is proposed. The model specifies a monomolecular layer in which disaccharide units are each 1.03 nm long, and the polysaccharide chains, all parallel, are 1.25 nm apart. The cross-linking peptide side-chains have the same atomic coordinates and are arranged above or below the polysaccharide chains.

Amino Acids↗