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Biomedical subjects

K Rytwiński

Publications and source records attributed to K Rytwiński.

9 recordsLinked to original sources
PubMed1992

[Anemia and recurrences in acute lymphoblastic leukemia in children after the treatment and the frequency of the erythroblasts with micronuclei].

A retrospective analysis of the relation of relapses and anaemias to the frequency of micronucleated erythroblasts in bone marrow smear in 140 children with acute lymphoblastic leukaemia after BFM and Memphis schemes of therapy was carried out. The anaemias correlated with the frequency of micronucleated erythroblasts in statistically significant manner. Correlation concerning relapses, was not shown. The frequency of micronucleated erythroblasts in children treated by BFM scheme is lower than by Memphis scheme. This suggests smaller genotoxicity of BFM scheme therapy.

Adolescent↗
PubMed1989

[Selected immunologic parameters and infections in children in over 1 year after completion of the treatment of acute lymphoblastic leukemia].

An analysis of infection incidence within one year after completion of the treatment for the acute lymphoblastic leukemia was performed in the group of 24 children. Infections incidence was related to the selected immunological parameters, mainly T4 and T8 lymphocyte subpopulations assayed with monoclonal antibodies. T4/T8 cells ratio (Ix) was determined. It was found that moderate decrease in the total lymphocyte count and Ix exist in children one year after completion of the treatment. This index is more significantly lower in children with more frequent infections while the absolute numbers of T4 and T8 lymphocytes are relatively increased. The obtained results suggest that no significant immunosuppression is observed in children affecting the number and the course of infections.

Bacterial Infections↗
PubMed1979

Adoptive immunotherapy and chemo-immunotherapy in murine L-1210 leukemia and its influence on the kinetics of leukemic proliferation.

Donor allogeneic C57Bl/6 lymphoid cells from peritoneal cavity, lymph nodes, thymus and spleen of immunized and non-immunized mice were used for adoptive immunotherapy of L-1210 ascites leukemia in DBA/2J recipients. Donor effector cells were injected i.p. into leukemic mice which were given a whole-body irradiation in a dose of 300 r X-rays prior to leukemia inoculation. The in vitro cytotoxicity of the effector cells was tested by the LT- and 51Cr-tests. Cytokinetics of leukemia undergoing therapy was followed by impulse-cytophotometry. An adoptive chemo-immunotherapy with the aid of peritoneal or splenic lymphoid cells from immunized donors proved to be most effective. The cells were injected on the second day into recipients inoculated with leukemia on the day 0, and then given on the first day a single i.p. injection of cyclophosphamide in a dose of 50 mg/kg body weight. Under these conditions the secondary disease did not influence therapy and apart from a distinctly prolonged survival there was also noted a number of permanent survivals of leukemia. It was also found that peritoneal and splenic lymphoid cells of immunized donors exhibited most pronounced direct and indirect cytotoxicity against target cells in vitro. In vivo, the effector cells mounted an attack on the L-1210 cells probably at the onset of the G1-phase of the cell cycle.

Animals↗
PubMed1976

[The acid-base equilibrium during mouse lymphoblastic leukemia].

In transplantable and in spontaneous lymphoblastic mouse leukemia blood pH, PCO2, TCO2 and BB were examined. Spontaneous Gross-leukemia in AKR mice was found to develop in its final phase respiratory acidosis. Transplantable (TAL) leukemia of AKR mice presents from the onset a tendency toward respiratory acidosis. "L-1210"-leukemia, on the contrary, was shown to alcalise the recipients during the first 8 days after inoculation, later on it can bring about an acidosis. The pH-deviations in "L-1210"-leukemia display a respiratory character in syngeneic DBA/2J recipients, whereas in semiallogeneic recipients the observed changes are metabolic or mixt in nature. This finding strongly argues for the importance of histocompatibility. Intranodally inoculated animals distinctly differ in their parameters in comparison to intravenously and intraperitoneally recipients, hence, an important role of the tissular-milieu which is put first in contact with the leukemic factor must be concluded.

AKR murine leukemia virus↗
PubMed1976

[The effect of exogenous alkalization on cytokinetics and on the survival rate of mice with lymphoblastic leukemia].

In mice vaccinated with two forms of lymphoblastic leukaemia and alkalized with intravenous administration of sodium bicarbonate, the survival rate, the extent of leukaemic infiltration and the proliferative capacity of cells in the bone-marrow, thymus, spleen, lymphnodes, liver and lungs were investigated. The survival rate in the TAL leukaemia of the AKR stem producing an endogenous acidosis could be significantly prolonged in a statistical way by alkalization. Yet an accelerated expiring rate could be observed after exogenous alkalization in L-1210 leukaemia of the DBA/2J stem producing an endogenous alkalosis. By means of cytological and impulse-cytophotometrical investigations the exogenous alkalization of both forms of leukaemia could be proved to have a direct bearing on the proliferative kinetics. In TAL leukaemia the leukaemic proliferation was inhibited by the exogenously involved correction of the acid-base balance; in the L-1210 leukaemia, however, the pH disturbances were enhanced, thus accelerating the leukaemic proliferation. Consequently, the disturbances of the acid base balance seem to be an essential cofactor in the leukaemia genesis. The exogenous direction of the acid-base balance may be important as a means of treating leukaemia.

Animals↗
PubMed1975

[A case of atypical myeloblasts; a RNA leukemia].

In a 53-year-old female patient, a tumor localized in the nasopharyngeal cavity together with hematologic features of acute paramyeloblastic leukemia were observed. More than 70% blast cells contained giant intracytoplasmic inclusions which have been found to be strongly pyroninophilic. The electron-microscopic study revealed big agglomerations of ribosomal RNA inside of pseudofibrillar structures of the cytoplasma. The possibility of a new nosological entity should be envisaged.

Bone Marrow↗
PubMedDate not supplied

Multiple immune serum injections in prevention of murine "L-1210" leukemia growth.

Adult BDF1 and DBA/2J mice were inoculated i.p. with "L-1210" leukemia cells and then received i.p. control or immune sera raised in C27B1/6 and BALB/c mice. Undiluted sera were administered in single or multiple doses ranging from 0.2 to 0.4 ml/mouse on day 0., resp. 0., 2-4, and 7. In BDF1 hybrids permanent survivals (greater than 150 days) and distinct prolongation of the mean survival time (MST) after multiple injections were observed. However, normal serum derived from non-immunized C57B1/6 donors was also demonstrated to provide protection when injected three times. In DBA/2J recipients no permanent survivals were observed. In this mouse strain control sera proved ineffective. On the contrary, immune sera enabled the recipients to survive longer and this prolongation proved to be statistically significant (p less than 0.02). Partial natural immunity against "L-1210" leukemia in BDF1 hybrids must be, therefore, postulated. It is probably due to H-2-locus incompatibility versus "L-1210" cells, inherited from the C57B1/6 ancetor-line. Unknown factors which are present in normal serum seem to potientiate this natural resistance. In compatible DBA/2J mice normal serum constituents were ineffective, on the other hand, however, the effectiveness of specific immune factors directed against target cells of the tumor could be demonstrated in them

Animals↗
PubMedDate not supplied

[Study of expression of surface antigen p72 using monoclonal antibody 79IT/36 and the effect of ricin A chain-bound 79IT/36 on phytohemagglutinin-stimulated human lymphocytes].

Monoclonal antibody 791T/36 directed against surface antigen on the human osteosarcoma cell line and cross-reacting with surface antigen p72 presenting on human PHA-stimulated T-lymphoblasts was used in analysis of p72 antigen expression on human mononuclear peripheral blood cells, between 2-4 days of culture. Using FACS-IV system and fluorescence microscope, it was shown that expression of p72-antigen is dependent on PHA-stimulation and the ability of lymphocytes proliferation. The expression of p72 preceding the entry of the cells into the cell cycle. This is not significantly dependent of cell size, stage of the cycle, and RNA-transcription activity. In PHA-stimulated cultures, between 2-4 days, the number of lymphoblasts expressing enough receptors for 791T/36 monoclonal antibody is sufficient to exhibit a distinct effect of Ricin A-chain conjugated with 791T/36 over 50% inhibition of 3H-Thymidine incorporation into the cells. These observations emphasises the importance of cross-reactions in cases using immunotoxins.

Antibodies, Monoclonal↗