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Biomedical subjects

K S Raghavan

Publications and source records attributed to K S Raghavan.

29 records · Page 2Linked to original sources
PubMed1978

Radioimmunoassay of oxytocin.

The evaluation of a radioimmunoassay of oxytocin is described. The method involved careful collection and transportation of blood at 4 degrees C, acidification of the plasma, extraction with Fuller's earth and radioimmunoassay using antisera raised in rabbits immunized against oxytocin conjugated to bovine serum albumin and 125I-labelled oxytocin. The antisera showed insignificant cross-reaction with a variety of small peptides including vasopressin and vasotocin. The limit of detection of the assay was 2.5 pg with intra-assay and interassay coefficients of variation of 7-15% and 12-18% respectively. Seventy-seven per cent (88 out of 116) of the pregnant women tested had detectable maternal plasma oxytocin. Serial samples of maternal plasma showed a significant increase in oxytocin from the first to the second stage of labour and a significant decrease in the third stage. Oxytocin concentrations in the umbilical arterial plasma were significantly higher in patients in labour. The significance of these findings is discussed.

Antibody Specificity↗
PubMed1975

Effect of prostaglandin F2 alpha and hypertonic saline on the placental function during midtrimester abortion.

Changes in placental function during the induction of midtrimester abortion by prostaglandin F2 alpha and hypertonic saline were investigated. Serial estimations of human placental lactogen (HPL) and cystine aminopeptidase (CAP) were carried out in 17 subjects. There was a significant (P smaller than 0.01) reduction in the plasma CAP and HPL levels 4 hours after PGF2 alpha administration. In the case of hypertonic saline abortions only HPL levels showed a reduction at 8 and 12 hours after induction.

Abortion, Induced↗