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Biomedical subjects

K S Shin

Publications and source records attributed to K S Shin.

At least 55 records · Page 3Linked to original sources
PubMed1996

Rhomboid design for tubed inguinal flap in fingertip reconstruction.

The authors present a rhomboid design for the tubed inguinal flap mainly nourished by the superficial circumflex iliac vessels and the superficial inferior epigastric vessels. The length and width of the flap are mathematically determined based on the perimeter and length of the fingertip soft-tissue defect, respectively. The rhomboid tubed inguinal flap was used in the treatment of 21 patients with a variety of fingertip injuries. The thumb was involved in 12 patients and other digits in 9 patients. A complication, flap necrosis, occurred in one obese female patient and osteomyelitis of an amputated phalangeal bone developed in another. In all other patients, the rhomboid tubed inguinal flap healed uneventfully and provided satisfying results with minimal donor site morbidity. As a secondary procedure in thumb injuries, four neurovascular island flaps and one wrap around flap from the great toe were done for improved sensation. The precisely tailored rhomboid flap, with a safe and abundant vascular pedicle, allows for effective reconstruction of the fingertip with easy primary closure and minimal morbidity of the donor site.

Adult↗
PubMed1995

Genotype distribution and comparison of the putative envelope region of hepatitis C virus from Korean patients.

Comparative nucleotide sequence studies of the genomes of hepatitis C virus (HCV) revealed that there are at least 6 different genotypes of HCV. The prevalence of HCV genotypes among the patients with liver diseases in Korea was investigated using the polymerase chain reaction (PCR) for the NS5 region. In the 75 HCV RNA positive samples, two genotypes, type 1b and type 2a, were the major causative agents which accounted for 60% and 33% of infections respectively, while 7% could not be assigned a genotype by the methods used. The nucleotide sequences of cDNAs encoding the putative envelope proteins from 10 type 1b and 5 type 2a genotype samples were analyzed. Approximately 31-42% of the nucleotide sequences of type 1b samples examined differed from those of different genotypes. In the case of type 2a samples, 36-42% of the nucleotide sequences differed from those of different genotypes. The diversities of the amino acid sequences were the same or greater than those of the nucleotide sequences. Two hypervariable regions (HVR1 and HVR2) were recognized in both HCV genomes of genotypes 1b and 2a. However, the sequence divergence within the HVR2 region of genotype 2a was less than that of genotype 1b.

Amino Acid Sequence↗
PubMed1995

Involvement of transglutaminase in myofibril assembly of chick embryonic myoblasts in culture.

Involvement of transglutaminase in myofibrillogenesis of chick embryonic myoblasts has been investigated in vitro. Both the activity and protein level of transglutaminase initially decreased to a minimal level at the time of burst of myoblast fusion but gradually increased thereafter. The localization of transglutaminase underwent a dramatic change from the whole cytoplasm in a diffuse pattern to the cross-striated sarcomeric A band, being strictly colocalized with the myosin thick filaments. For a brief period prior to the appearance of cross-striation, transglutaminase was localized in nonstriated filamental structures that coincided with the stress fiber-like structures. When 12-o-tetradecanoyl phorbol acetate was added to muscle cell cultures to induce the sequential disassembly of thin and thick filaments, transglutaminase was strictly colocalized with the myosin thick filaments even in the myosacs, of which most of the thin filaments were disrupted. Moreover, monodansylcadaverine, a competitive inhibitor of transglutaminase, reversibly inhibited the myofibril maturation. In addition, myosin heavy chain behaved as one of the potential intracellular substrates for transglutaminase. The cross-linked myosin complex constituted approximately 5% of the total Triton X-100-insoluble pool of myosin molecules in developing muscle cells, and its level was reduced to below 1% upon treatment with monodansylcadaverine. These results suggest that transglutaminase plays a crucial role in myofibrillogenesis of developing chick skeletal muscle.

Animals↗
PubMed1995

An improved burying method for salvaging an amputated auricular cartilage.

An improved burying method for salvaging an amputated auricle is presented. We elevated a mastoid skin flap and a fascial flap simultaneously and inserted the denuded auricular cartilage between them. This procedure can maintain the auricular cartilage in a more natural form by eliminating undue skin tension and can give a favorable aesthetic result.

Adult↗
PubMed1994

A candidate molecule for the matrix assembly receptor to the N-terminal 29-kDa fragment of fibronectin in chick myoblasts.

Myoblast surface proteins with binding activity toward the N-terminal 29-kDa fragment of fibronectin were identified by two different experimental techniques: one involves radioiodination of the cell surface proteins, followed by solubilization with Triton X-100 and affinity purification on a Sepharose column conjugated with the 29-kDa fragment, and the other involves cross-linking of the 29-kDa fragment to the cells metabolically labeled with [35S]methionine, followed by immunoprecipitation with anti-29-kDa IgG. Both approaches revealed that primary cultures of chick myoblasts contain the 66- and 48-kDa proteins that bind to the 29-kDa fragment. These binding proteins were then purified to apparent homogeneity by two successive chromatographies of the solubilized extracts of 12-day-old embryonic muscle on wheat germ agglutinin-agarose and 29-kDa fragment-Sepharose columns. However, the 48-kDa protein was found to be derived from contaminating fibroblasts upon immunoblot analysis of the myogenic cell lines, rat L8E63 and mouse C2A3, and cultured fibroblasts using the antibody raised against the 66-kDa protein. Anti-66-kDa IgG inhibited the binding of the 125I-29-kDa protein to the primary culture of myoblasts in a dose-dependent manner. On the other hand, the same antibody showed little or no effect on the initial binding of 125I-fibronectin to the cell surface, but dramatically inhibited its incorporation into deoxycholate-insoluble matrices. Furthermore, Fab fragments of anti-66-kDa IgG completely blocked the incorporation of fluoresceinated fibronectin into matrices but not its binding to the cell surface. These results suggest that fibronectin matrix assembly is mediated at least in part by the interaction of the 66-kDa protein with the N-terminal type I domain of fibronectin.

Amino Acid Sequence↗
PubMed1994

Columella lengthening in nasal tip plasty of Orientals.

To elevate the nasal tip in Orientals, two simple adjunctive procedures are presented. Either V-Y advancement or earlobe composite graft can be used after the open rhinoplasty incision on the columella-labial junction. Over the past 6 years, 62 Oriental patients with short columella were treated with one of the above procedures. The nasal tip projection as well as nasal tip appearance greatly improved. The scar in the columellar base posed no problems, and none of the patients complained about the scar. The procedures are particularly useful for cosmetic rhinoplasty in Orientals as well as in cleft lip nasal deformity.

Cleft Lip↗
PubMed1993

Purification and characterisation of D-glucose oxidase from white-rot fungus Pleurotus ostreatus.

D-Glucose oxidase was purified 27.5-fold to apparent homogeneity with an overall yield of 23.8%, from Pleurotus ostreatus, through a purification procedure of ammonium sulphate precipitation, gel-permeation, anion-exchange and hydrophobic-interaction chromatography. The molecular mass determined by gel filtration was found to be 290 kDa. SDS/PAGE revealed that the enzyme consists of four subunits with a molecular mass of 70 kDa. The absorption spectra of the enzyme exhibit maxima at 280, 360 and 460 nm. The enzyme shows a fluorescence spectrum with an excitation maximum at 470 nm and an emission maximum at 530 nm. These results indicate that the prosthetic group of the enzyme is flavin and that the enzyme contains 4 mol flavin/mol enzyme. The enzyme is optimally active at 50 degrees C and at pH 5.5-6.0. It exhibits broad affinity for various sugars and specificity for D-glucose with Km value of 1.34 mM. 2,6-Dichloroindophenol, Wurster's blue, and 4-benzoquinone can function as electron acceptors but phenazine methosulphate cannot function as an electron acceptor. The enzyme is inhibited completely by mercuric chloride and partially by silver sulphate, sodium azide 8-hydroxyquinoline.

Amino Acids↗
PubMed1993

Purification and characterisation of an extracellular peroxidase from white-rot fungus Pleurotus ostreatus.

A peroxidase was purified 98.3-fold from the culture filtrate of Pleurotus ostreatus with an overall yield of 12.4%. The molecular mass determined by gel filtration was found to be approx. 140 kDa. SDS-PAGE revealed that the enzyme consists of two identical subunits with a molecular mass of approx. 72 kDa. The pI value of this enzyme is approx. 4.3. The enzyme contains 41% carbohydrate by weight, and aspartic acid and asparagine (16.8%), and glutamic acid and glutamine (12.0%). The enzyme has the highest affinity toward synaptic acid and affinity towards various phenolic compounds containing methoxyl and p-hydroxyl groups, directly attached to the benzene ring. However, the enzyme does not react with veratryl alcohol and shows no affinity for nonphenolic compounds. The optimal reaction pH and temperature are 4.0 and 40 degrees C, respectively. The catalytic mechanism of the enzymic reaction is of the Ping-Pong type. The activity of the enzyme is competitively inhibited by high concentrations of H2O2 and its Ki value is 1.70 mM against H2O2. This enzyme contains approx. 1 mol of heme per mol of one subunit of the enzyme. The pyridine hemochrome spectrum of the enzyme indicates that the heme of P. ostreatus peroxidase is iron protoporphyrin IX. The EPR spectrum of the native peroxidase shows the presence of a high-spin ferric complex with g values at 6.102, 5.643 and 1.991.

Amino Acids↗
PubMed1992

Reduction mammoplasty with the short submammary scar (S-S-S) technique.

Since its introduction, reduction mammoplasty has undergone a process of evolution and improvement that affects its three surgical goals: (1) safe transposition of the nipple-areola complex, (2) sufficient reduction of volume, and (3) aesthetically acceptable results. Unfortunately, most reduction mammoplasty techniques still result in a long transverse scar on the chest wall which often presents a problem, particularly a hypertrophic condition. In 1957 Arie proposed a vertical scar technique for mastopexy; recently Lassus, Marchac, Peixoto, d'Angieri Basile, and others reported their original methods that try to reduce the length of the transverse scar. We modified these previously proposed techniques and present our experiences with a reduction mammoplasty called the short submammary scar (S-S-S) technique.

Adult↗
PubMed1991

The fasciovascular pedicle for revascularization of other tissues.

A fasciovascular pedicle based on the epigastric vessels was developed in a rat model to determine if it could be used as a "universal carrier" to revascularize a new composite flap. The effects of time course, carrier size, and flap ischemia on the revascularization process were studied. A 2.5 x 4-cm or 1 x 4-cm fascial patch pedicled on the vessels was transferred under bipedicled 2.5 x 4-, 6-, or 8-cm abdominal panniculocutaneous flaps. At different time intervals, the flap was raised as an island flap connected only by it vascular bundle and then sutured back in place. The skin perfusion by dermofluorometry and flap survival were both markedly increased on day 5 (p less than 0.001). The wide carrier had a 93% survival area, whereas the narrow carrier had only 71%. The wide carrier induced relatively faster and better revascularization (p less than 0.05). Moderate ischemia promoted revascularization (p less than 0.01). An india ink injection study and histological examination provided visual evidence of revascularization. This fasciovascular pedicle is a promising model for prefabrication of complex new composite flaps and for studying the process of revascularization between the layers. Based on these findings and further investigations, a thin, prefabricated abdominal free flap was successfully transferred for facial resurfacing in humans.

Abdomen↗
PubMed1991

Total palmar resurfacing with scapular free flap in a 26-year contracted hand.

The scapular free flap offers a large-sized and well-vascularized coverage for variable defects. It is not regarded, however, as a favorite tool for palmar resurfacing because of its bulky and rigid nature. A 28-year-old man had sustained a contact dermal injury at the age of 2 years that resulted in a severely deformed hand. The total palmar defect was resurfaced by surgical intervention with a scapular free flap. After secondary procedures, the final result was promising. The present report focuses on the secondary procedures, which make a more functional and aesthetically pleasing hand after coverage with the scapular flap. Also, our result suggests that even finger joints contracted for 26 years can recover motion if they have not been directly damaged.

Adult↗
PubMed1991

Functioning free latissimus dorsi muscle transplantation: anterogradely positioned usage in reconstruction of extensive forearm defect.

We present a patient with successful functional recovery of a severely injured upper extremity after a microneurovascular free latissimus dorsi muscle and combined composite skin were transplanted with an anterograde position. In our patient, the anterogradely positioned usage of the lastissimus muscle offered two major advantages; one advantage was the direct anastomosis of a more proximal healthy uninjured recipient nerve without interpositional nerve graft, and the other, one-stage coverage including the elbow wound with combined scapular skin flap. From our result, we deduce that a neurovascular free latissimus dorsi muscle transplantation with an anterograde position is selectively indicated in functional recovery of an extensively injured forearm.

Adult↗
PubMed1991

A single-stage two-flap method of total ear reconstruction.

A single-stage two-flap method of total ear reconstruction in congenital microtia is reported. This method was derived from the one-stage reconstruction described by Song and Song. Two flaps defined by vascular basis were elevated on the mastoid area: the superficial skin flap supplied mainly by subcutaneous pedicled arteriole perforators from the posterior auricular artery and the deeper axial-pattern fascial flap including the posterior auricular artery itself. The ear framework, exaggeratedly carved using autologous rib cartilage, could be inserted easily between the two flaps, simultaneously producing the auriculocephalic angle and the conchal wall. Intraoperative expansion of the skin flap and postoperative external ear molding also were performed to create aesthetically pleasing ears.

Adolescent↗
PubMed1989

Behçet's disease with Sweet's syndrome-like presentation--a report of six cases.

We describe six cases of Behcet's disease, which showed Sweet's syndrome-like features. The clinical findings in our patients closely resemble those of the cases described in previous reports on Sweet's syndrome. However, oral and genital mucosal involvement, non-tenderness of the lesions and a pustular eruption elsewhere made us consider Behçet's disease. Besides the clinical findings, vasculitis was clearly demonstrated in the skin biopsy specimens.

Adult↗
PubMed1988

Restoration of finger extension and forearm contour utilizing a neurovascular latissimus dorsi free flap.

The use of a free neurovascular latissimus dorsi flap for restoration of wrist and finger extension and forearm contour is described in a burn patient. Wound coverage is the prime need of the extensive tissue loss in the upper limb, and a muscle or musculocutaneous flap is necessary to cover the exposed bone and muscles for infection control. Furthermore, restoration of the limb function as well as aesthetic flap donor and recipient site contour are needed. The case presented is the extremely rare patient in whom restoration of finger extension was achieved after the nearly total loss of the extensor forearm muscles.

Adolescent↗
PubMed1988

A new arterial flap from the postauricular surface: its anatomic basis and clinical application.

We studied the arterial pattern of the postauricular surface in 16 cadavers and compared them to the results of Doppler probing in the auricles of 15 human volunteers. The dissections revealed that the postauricular surface is mainly supplied by three to five sizable branches from the upper, middle, and lower divisions of the posterior auricular artery, traversing in a rather straight and cephalic direction toward the auricular rim. One or more branches of the middle division are constantly present and distribute over the upper two-thirds of the postauricular surface. These anatomic findings were compatible with the results of the Doppler probing. We have done four auricular reconstructions with postauricular arterial flaps with excellent postoperative results. It is worthwhile to use the postauricular arterial flap in restoring the middle third of the auricle.

Adult↗
PubMed1987

Nostril sill augmentation in secondary cleft lip.

In most cases, after successful correction of primary cleft lip deformity, some problems remain to be solved. One of them is nostril sill depression, which should not be overlooked in cleft lip repair. However, there have been few reports on the anatomy of the depressed nostril sill and methods for augmenting the sill depression. The anatomy of the depressed nostril sill, its relationship to surrounding tissue, and the gross features of nostril sill depression in cleft lip deformity were reviewed, and a method in which a scar flap and a suture-fixation technique are used was developed for augmenting the depression. The clinical results of this method in 16 patients are presented.

Cicatrix↗