The interfering effect of serum in the study of lymphocyte interactions in vivo and in vitro.
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Blood Proteins↗
Biomedical subjects
K Sůla
Publications and source records attributed to K Sůla.
Dissociation of the proliferative and effector non-H-2 minor histocompatibility-alloimmune responses.
The first-set and second-set allotransplantation reactions against skin grafts and the primary and secondary proliferative graft-versus-host reactions in the popliteal lymph nodes were compared in both directions in a non-H-2 system (mouse strain combinations C57BL/10ScSnPh (further B10) and B10.C3H(40NX) further 40NX) differing at H-1 plus H-?). While 40NX recipients gave stronger reactions against B10 antigens in the allotransplantation reactions, the situation was reversed in the GVHR, B10 cells reacting more strongly against 40NX antigens. The findings of a dissociation between the mechanisms of allotransplantation reaction adn proliferative GVHR suggest that the genetic determination of the target antigens and the reacting lymphocyte populations are more complex at the minor histocompatibility systems than has been expected.
Animals↗
Functional differentiation of mouse T lymphocytes. GVHR-precursors: tissue origin and specificity of activity inducer.
For theoretical and practical reasons, it is important to find out whether the differentiation of T cell precursors to the functional lymphocytes can be induced under in vitro conditions. Using the local GVHR assay (based on the enlargement of the popliteal lymph node), the inducibility of the precursors of reactive cells was studied with bone marrow, thymus, spleen, and lymph node cell suspensions submitted to short-term incubation with cell-free extracts from calf thymus, spleen or brain. GVHR-precursors from bone marrow were inducible not only specifically (i.e., with thymus extract) but also--and even to a higher degree--with spleen or brain extract. Thymus and spleen cell suspensions (the latter also depleted of the reactive subpopulation by treatment with anti-Thy 1.2 serum and complement) were, on the other hand, inducible mainly specifically, whereas lymph node cells were refractory to induction. The inductive action of tissue extracts obviously depends on the tissue origin of T cell precursors; their effects on pre- and postthymic differentiation of T lymphocytes are discussed.
Animals↗
HLA--Dw3 in Sjögren's syndrome.
The increased frequency of HLA-B8 in Sjögren's syndrome was recently reported independently by Gershwin et al. (1975) and Iványi et al. (1976). The association of HLA-B8 antigen with other diseases, characterized generally by impaired immunologic reactivity, has been shown (see Svejgaard et al. 1975, Dausset & Hors 1975), and in some of these diseases MLC typing has revealed the strong association also with the Dw3 determinant. The degree of association with each of the two, B8 and Dw3, varied between different diseases, in some of them having been reported to be higher for antigen B8 (Möller et al. 1976) and in the others for the determinant Dw3 (Thomsen et al. 1975, 1976). In some of the diseases, the association was found to be equally strong for both determinants (Solheim et al. 1976, Thorsby et al. 1975). In this communication, we describe the typing of HLA-Dw3 in 29 patients with Sjögren's syndrome (Ss). They represent part of the experimental group reported in a previous study (Iványi et al. 1976).
HLA Antigens↗
Alloimmune anti-H-2 mouse sera inhibit human mixed lymphocyte reactions.
The inhibitory effect of alloimmune congenic mouse anti-H-2f serum on human mixed lymphocyte cultures was investigated. The anti-H-2 serum tested produced a strong inhibitory effect on human allogeneic MLR. This effect was more pronounced when HLA-A2 positive cells were involved in the MLR. More experiments are needed to substantiate the finding that both stimulator and responder functions of the human lymphocytes are impaired. The anti-H-2 serum also inhibited the human lymphocyte response to PHA.
Animals↗