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Biomedical subjects

K Sakakida

Publications and source records attributed to K Sakakida.

11 recordsLinked to original sources
PubMed1990

Experiments on antigenicity and osteogenicity in allotransplanted cancellous bone.

This experimental study was undertaken in mice to investigate the procedures for storage of allogenic cancellous bone. Cancellous grafts were were stored at -80 degrees C, -196 degrees C or freeze dried. Grafts were implanted into a defect in the recipient's femur and after one week the cellular kinetic activity was analysed by autoradiography. For immunological study, the grafts were implanted into the recipient's muscle, and 2 weeks later the sensitising properties were examined by killer T-cell toxicity and the complement dependent cytotoxicity test. The capacity for osteogenesis of grafts frozen at -196 degrees C was similar to or higher than those freeze dried, but it was low when storage was at -80 degrees C. Immunogenicity was not affected by these three methods of storage and was very similar to that of fresh autografts. Antigenicity of allografts can be decreased by the freezing procedure. We suggest that the greater osteogenic potency after freezing at 196 degrees C is related to the lesser degree of degeneration of the bone matrix.

Animals↗
PubMed1990

Experimental studies on half-joint transplantation. Antigenicity of fresh allografted articular cartilage and changes in the articular cartilage.

This study was undertaken to examine the fate of articular cartilage in isogenic half-joint grafts implanted after storage by two-stage freezing. The antigenicity was evaluated quantitatively with regard to humoral and cellular immunological responses: none was seen in allogenic cartilage grafts. In the half-joint transplantations, the antigenicity of the cartilage need not be considered; only the antigenicity of cortical and cancellous bone must be reduced. Histologically, half-joint grafts stored at -196 degrees C were superior to fresh allografts. Two-stage freezing, therefore, appears to be useful for the storage of articular cartilage.

Animals↗
PubMed1990

[A preliminary study on the morphological changes of the microvasculature following the peripheral nerve autografting].

The microvasculature of peripheral nerve following autografting was studied by scanning electron microscopy. The newly-formed capillaries was also examined by transmission electron microscopy. Capillaries of a fascicle were divided into an extrafascicular vascular plexus and an intrafascicular capillary bed; the former capillaries formed networks, while the latter capillaries ran longitudinally and formed slightly spiral curve. Normal intrafascicular capillaries had tight junctions, a continuous basement membrane and pericytes. On the suture line, capillaries of the graft and those of the host were connected by newly developed sinusoidal capillaries, which were formed at the extrafacicular region earlier than at the intrafascicular region. Newly developed sinusoidal capillaries were transformed into glomerulus-like capillaries and formed fine networks. These networks exhibited a normal capillary architecture after reconstruction. The endothelium of newly-formed capillaries showed a discontinuous basement membrane at the early stage of regeneration. An intrafascicular capillary bed was present in the middle part of the graft.

Animals↗
PubMed1989

The recovery of growth cartilage after removal of bone bridge between epiphysis and metaphysis.

The in vitro proliferation of chondrocytes from the tibial growth plate of rabbits has been examined following bone bridge formation and its resection in vivo. In vitro 3H-thymidine incorporation by growth cartilage with bone bridges was 53.5% of the contralateral normal growth cartilage, whereas it was 95.7%, following removal of the bone bridges performed five days before sacrifice. Relative 3H-thymidine incorporation into the growth cartilage decreased with increasing discrepancy of tibial length caused by persisting bone bridges. These experiments demonstrated that growth cartilage can recover its proliferative potential following removal of a mechanical growth inhibition caused by the formation of a bone bridge.

Animals↗
PubMed1989

Concentration of interleukin-1 beta in serum and synovial fluid in patients with rheumatoid arthritis and those with osteoarthritis.

The concentration of interleukin-1 beta (IL-1 beta) in serum and synovial fluid of the knee of patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA) was examined using a recently developed radioimmunoassay method. The serum concentration of IL-1 beta was below the detection limit of 0.25 ng/ml in all RA and OA patients. The IL-1 beta concentration in the synovial fluid was higher in RA patients than in OA patients. In RA patients, a significant correlation was demonstrated between the synovial fluid IL-1 beta concentration and the erythrocyte sedimentation rate, C-reactive protein level, synovial fluid white blood cell count and Lansbury index. The synovial fluid IL-1 beta concentration decreased with the reduction of the rheumatoid activity by the medication, but tended to increase or remain high when inflammatory activity of RA could not be adequately controlled. The IL-1 beta concentration, easily determined, shows promise as a new quantitative indicator of rheumatoid activity.

Aged↗
PubMed1989

Immunohistochemical localization of interleukin 1 in human growth cartilage.

Although it has been reported that interleukin 1 (IL-1) stimulate chondrocytes to produce collagenase and proteoglycanase in vitro, IL-1 producing cells and the function of IL-1 have not been demonstrated in osteocartilaginous tissue in vivo. Immunohistochemical studies of human cartilaginous epiphysis and growth cartilage demonstrated that IL-1 was detected in: (1) chondrocytes surrounding cartilage canal, (2) hypertrophic chondrocytes in cartilaginous epiphysis, (3) chondrocytes at the hypertrophic and calcified zones in the growth cartilage of actively growing bone. In contrast, few hypertrophic chondrocytes showed positive reactions to IL-1 in growth plates nearing physiologic closure. Furthermore, IL-1 was detected in chondrocytes cultured from human growth cartilage. These results show that IL-1 is produced by matured chondrocytes of human growth cartilage in vivo. Chondrocyte-derived IL-1 might play a key role in the hypertrophy of chondrocytes, in the vascularization of cartilage and in the formation of bone.

Adolescent↗
PubMedDate not supplied

Evaluation of arthrography and arthroscopy for lesions of the posteromedial corner of the knee.

Injuries of the posteromedial corner of the knee joint were evaluated using the results of clinical, arthrographic, and arthroscopic examinations in 137 surgically treated patients (137 knees) with injuries of the ACL (acute in 61, chronic in 76). The accuracy of each diagnosis was confirmed by determining the site of the injury during the arthrotomy. A tear of the posteromedial corner of the medial meniscus was present in 40 patients, and an accurate diagnosis was made in 29 patients by clinical examination, in all 40 patients by arthrography, and in 38 patients by arthroscopy. Injury of the posterior oblique ligament was noted in 37 patients, and an accurate diagnosis was made in 15 patients by clinical examination, in 35 patients by arthrography, and in 8 patients by arthroscopy. Lesions of the posterior oblique ligament which were not detected in the arthroscopic examination alone were either rupture at the attachment to the femur or longitudinal tears in the meniscofemoral or meniscotibial portion. The results revealed that arthrography is an important means of evaluation in the diagnosis of injuries of the posteromedial corner of the knee joint.

Adolescent↗
PubMedDate not supplied

[External fixation with autopolymerizing acrylic resin].

In November 1970, a new method of skeletal fixation was devised using self-curing acrylic resin. Manipulative reduction of the displaced fracture is made using screws inserted into the bone fragments under an image intensifier. After reduction, the proximal and distal fragments are joined together with the aid of self-curing acrylic resin. Adjacent joints can be exercised immediately after operation and the fixation is so firm that the use of a plaster cast after operation is not necessary. This simple method can be used in osteosynthesis, compression arthrodesis and various types of osteotomy.

Acrylic Resins↗