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Biomedical subjects

K Schulz

Publications and source records attributed to K Schulz.

At least 73 records · Page 4Linked to original sources
PubMed1992

A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction.

The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.

Acquired Immunodeficiency Syndrome↗
PubMed1992

A recalcitrant, erythematous, desquamating disorder associated with toxin-producing staphylococci in patients with AIDS.

Although staphylococcal infections are common in patients with AIDS, staphylococcal toxin-related disorders have rarely been described. Five cases of a staphylococcal toxin-associated syndrome characterized by prolonged erythema, extensive cutaneous desquamation, hypotension, tachycardia, and multiple organ involvement are described in patients with AIDS. These illnesses were recurrent and recalcitrant with a mean duration of 50 days. Toxic shock syndrome toxin-1-producing staphylococci were isolated from three and staphylococcal enterotoxins B and A from one patient each. Sources of organisms were blood, one patient, and soft tissues and nasal accessory sinuses, two patients each. Three of the five patients died of renal failure and central nervous system abnormalities. One survivor required intubation for respiratory failure. All individuals manifested a marked diminution of CD4+ cells. Other laboratory abnormalities included azotemia and prolongation of partial thromboplastin time. Oliguria occurred in three patients. Thus, this recalcitrant erythematous desquamative disorder appears to be a variant of staphylococcal toxic shock syndrome in certain subsets of immunocompromised individuals.

Acquired Immunodeficiency Syndrome↗
PubMed1990

Comparison of capillary and plasma glucose values in screening and oral glucose tolerance testing in pregnancy.

The substitution of capillary reflectance meter glucose values for plasma or serum glucose values would be practical and cost saving. A direct comparison was performed of simultaneous capillary reflectance meter and plasma glucose values in routine screening and oral glucose tolerance testing in a pregnant population. The use of capillary glucose in 50-g glucola screening had a sensitivity of 82.6% and specificity of 98.1% as compared to the standard plasma method. Even though our data demonstrate a good correlation (r = 0.897) between capillary and plasma glucose values, our results do not support the direct substitution of plasma glucose values with capillary reflectance meter glucose values in screening for or diagnosing glucose intolerance in an obstetric population.

Blood Glucose↗
PubMed1990

[Risk profile and macroangiopathy in type II diabetics in medical practice].

The risk profile and the macro-vascular complications of patients with type II diabetes mellitus (NIDDM) was investigated in general practice patients for the first time in the FRG. It was the aim of the study to evaluate the efficacy of the therapy and possible improvements after detailed instructions in a random sample of well defined NIDDM in the greater Munich area. 290 NIDDM (187 female, 103 male) out of a total of 1500 patients treated by 22 general practitioners were randomly recruited for the study. First results indicated an excess morbidity of the NIDDM, e.g. 43.5% with HbA1c greater than 8%, hypertension in 73.8%, hypertriglyceridemia in 75%, hypercholesterolemia in 36.3% adipositas in 78%, and a micro/macro-albuminuria in 44.5%. A similar risk profile could be determined in cases with recently diagnosed NIDDM. The remarkable risk profile documents itself in the incidence of macro-vascular complications: 40.8% of the male and 43.2% of the female showed a peripheral arterial disease (pAVD), in 8% of all patients a carotid artery stenoses could be detected by means of doppler ultrasound technique; 46.6% of the male and 59.3% of the female patients showed symptoms of CHD. With the exception of the incidence of CHD in patients less than 64 years the duration of NIDDM had no influence on the macro-vascular complications as demonstrated in previous studies. The age however always had a significant influence on all three vascular regions examined. Albuminuria correlated as such with a number of risk factors showed a significant correlation with the incidence of pAVD and occurred more often in males with carotid artery stenoses. Other correlations established were: Hypercholesterolemia and FVIII ass. Ag respectively, and the incidence of carotid artery stenoses; blood pressure, F VIII ass. Ag and pAVD. In the female a negative correlation could be seen between the pAVD and the HDL-level. In patients with CHD sex specific correlations could be determined to blood pressure, HbA1c, c-peptide and triglyceride levels.

Adult↗
PubMed1989

Comparison of capillary Accu-Chek blood glucose values to laboratory values.

With 2 to 4% of the obstetric population demonstrating glucose intolerance, many authorities advocate the routine screening of all pregnant women. Some have questioned the cost effectiveness of such an approach and have chosen to screen only those with high-risk factors, overlooking a large percentage of gestational diabetics. The purpose of this report was to determine if Accu-Chek glucometer values are sufficiently accurate to substitute for laboratory values in our medical complex. Capillary Accu-Chek glucose values and laboratory values were compared in 140 patients. Values ranged from a low of 43 to a high of 380. The mean +/- SD glucose values were 142 +/- 73 mg/dl and 142 +/- 80 mg/dl, respectively. These values are not significantly different. Using a cutoff of 140 mg/dl as abnormal, the sensitivity of the capillary Accu-Chek was 100% and the specificity was 95% compared with the venous method. A simple linear regression indicated that there was a strong correlation between the capillary Accu-Chek glucose values and the laboratory glucose values (r = 0.95, p less than or equal to 0.0001). The slope was not different from 1 (p = 0.3135), and the intercept was not different from 0 (p = 0.4943), illustrating that there was an equality in the values and that one value may be substituted for the other.

Blood Glucose↗
PubMed1984

[Iron metabolism in chronic alcohol abuse].

Disturbances of the iron metabolism in patients with chronic alcohol abuse are of very complex genesis. The frequently probable hypersideraemia is caused by an increased intake of iron, by release of depot iron, when an alcohol-conditioned liver damage is present, and by an increased iron balance with disturbed iron use of the bone marrow. Thus the in alcoholics often provable combined megaloblastic-sideroblastic anaemia is in close connection with this.

Alcoholism↗
PubMed1983

[Hematologic changes following chronic alcoholism].

Changes in all three blood cell systems could be covered in 50 patients treated in hospital for a high consumption of alcohol for years and even decades. There is no symptom which would be pathognomonic for alcoholism in itself. Macrocytosis and macrovoluminity of erythrocytes, hyper-sideraemia and thrombocytopenia were findings frequently encountered and easily to be identified. Megaloblasts, vacuolization, and an increase of sideroblasts could be observed in the bone-marrow. The prompt reversibility of these changes mentioned by simply abstaining from alcohol has a considerable diagnostic utility. The impact of liver damage partly produced by an accompanying spleen enlargement could only be ensured for thrombocyte depression. The increase of methaemoglobin which is unequivocal but without any clinical importance can also be reversed by alcohol deprivation. From a haematological point of view an alcoholic is endangered by a deficient immunological system. Haemorrhagic diatheses due to thrombocytopenia, thromboembolic complications during rebound-thrombocytosis and severe haemolyses can rarely be found.

Adult↗