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Biomedical subjects

K Sh Nadareĭshvili

Publications and source records attributed to K Sh Nadareĭshvili.

17 recordsLinked to original sources
PubMedDate not supplied

[Properties of globular protein monolayers at physiological temperature intervals].

The dynamics of changes in monolayer (MML) static and kinematic characteristics of some globular proteins (humans' serum, albumin, actin, cytochrome c) was studied. Two series of experiments were carried out: 1) formation of MML at the stable temperature of subphase 12, 23 and 32 degrees C; 2) formation of MML at 12 degrees C and equilibrium warming up and following cooling of subphase with MML in the temperature interval of 12-34 degrees C. In the cooling-warming up cycle the dependence of MML surface pressure change on the temperature was found to have the form of reserved loop hysteresis with the minimum at 21 +/- 2 degrees C on warming up branch. By the following rise of temperature up to reaching the plateau (26 degrees C) the entropy of monolayer takes the negative meaning. On the branch of cooling loop such transition is not seen. These data indicate possibility of trigger conformative changes of protein molecules, which in situ condition can occur during the natural local (metabolic) temperature gradients in living cells and influence the functional specificity of protein molecules and their bioorganic complexes.

Actins↗
PubMedDate not supplied

[Postradiation changes in the activity of membrane-bound nerve tissue enzymes].

A study was made of the effect of various radiation doses (1.75 to 12.25 Gy) on the enzyme activity of Na,K-ATPase system of the microsomal brain fraction of mongrel and Wistar rats. With a similar method of the fraction isolation different response of the activity of this enzyme was registered. Different radiosensitivity of M9-ATPase is responsible for the direction of changes in the Na,K-ATPase activity of the preparations.

4-Nitrophenylphosphatase↗
PubMedDate not supplied

[The radiomodifying action of ATP and ADP on membrane-bound enzymes].

A modifying action of ATP and ADP on the activity of some key membrane-bound enzymes of the brain and heart microsomes of rats exposed to 7 Gy radiation has been investigated. The difference in the reactions of energy-dependent enzymes is attributed to the compensatory systems involved at the molecular level.

4-Nitrophenylphosphatase↗
PubMedDate not supplied

[Interaction of acetylcholine and tubocurarine with protein molecules of the actin complex and phosphatidylcholine].

By means of surface Wilhelmi weight type changes of monomolecular layer properties of some proteins and lipids at the air--water boundary have been studied during acetylcholine and tubocurarine injection in the subphase. Active interaction of acetylcholine and tubocurarine molecules with monolayers of actin, myosin and phosphatidylcholine was observed, while the properties of human serum albumin (HSA) and cholesterine were not influenced by the same physiologically active substances. These substances had no effect on the area of monolayers and on other physico-chemical parameters of HSA and cholesterine. On the other hand when acetylcholine is present in the subphase of phosphatidylcholine monolayer the velocity of HSA penetration in these monolayers in sharply decreased.

Acetylcholine↗
PubMedDate not supplied

[Blastocoele potential in the early frog embryo].

The resistance of intercellular gaps between the blastomeres and the potential of the blastocoel cavity in the cleaving frog eggs were estimated by means of microelectrode technique. The maximal and minimal values of these parameters were evaluated in different phases of the mitotic cycle.

Animals↗