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K Simpson

Publications and source records attributed to K Simpson.

At least 109 records · Page 6Linked to original sources

Use of reversed-phase high-performance liquid chromatographic analysis for the determination of provitamin A carotenes in tomatoes.

The usual methods for provitamin A evaluation of foods convert the total pigment amount, determined spectrophotometrically, into vitamin A units. Since the totally inactive lycopene is the major carotenoid in the tomato, such readings result in erroneously high provitamin A values. In view of the recent development of chemically bonded, reversed-phase, microparticulate packings and their use in high-performance liquid chromatography which combines highly accurate and reproducible resolution with the speed and ease of operation, a new method using such a system was developed to isolate carotenoid pigments from tomato samples. A 15-min column separation was thus achieved, dramatically decreasing the analysis time of the classical open column chromatographic procedures, which often result in unresolved and altered fractions due to long-term exposure to oxygen, light, solvents and sometimes adsorbent. beta-Carotene and lycopene were determined and quantitated in six tomato samples. beta-Carotene, 100% vitamin A-active, was expressed in International Units of vitamin A. The newly developed method gives a more reliable evaluation of the fruit potency in vitamin A than the methods of the Association of Official Analytical Chemists currently used for food composition tables.

Carotenoids↗

Neonatal respiratory failure due to myotonic dystrophy.

Myotonic dystrophy should be included in the differential diagnosis of neonatal respiratory failure accompanied by hypotonia. The effect of this disorder in an infant who died from it 49 hours after birth is described, and the importance of examining the mother of a possible case is emphasized.

Humans↗

The expert witness.

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Expert Testimony↗

Apicoectomy.

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Apicoectomy↗