SEQIN-ST and CODFREG: a full screen sequence editor and a codon usage analysis program for the Atari ST.
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Amino Acid Sequence↗
Biomedical subjects
K Stüber
Publications and source records attributed to K Stüber.
Recognition of ill-defined signals in nucleic acid sequences.
A set of programs has been developed for the definition and handling of nucleic acid sequence consensus information. The sequences of known genetic control signals are combined in a matrix. The origins and positions of the signals are recorded. Old matrices can be updated dynamically: new signals are included and obsolete ones deleted. Matrices of several different types are computed optionally. Several of these matrices can be combined to find possible new signals. The use of matrices allows the exact quantification of signal qualities. The described programs are part of a program library named GENEXPERT. Application examples given are the search for tRNA genes and the search for promoters in the bacteriophage lambda genome.
Algorithms↗
GENEXPERT, a program system for nucleic acid sequence structural interpretation.
A program system for nucleic acid sequence analysis has been developed. The detection and prediction of secondary structures in nucleic acids have been especially emphasized. The system is able to handle most of the common problems found with nucleic acid sequencing like testing homologies, searching for reading frames and signals etc., but in addition it is able to locate and predict secondary structures. The predicted structures can either be printed on normal line printers or displayed on drawing devices like plotters or video screens. Another special feature is the data base-like handling of consensus sequence matrices by the programs DYCOM and EAGLE. Detailed descriptions of the programs have been published elsewhere (Stüber, 1985, 1986).
Base Sequence↗
Nucleic acid secondary structure prediction and display.
A set of programs has been developed for the prediction and display of nucleic acid secondary structures. Information from experimental data can be used to restrict or enforce secondary structural elements. The predictions can be displayed either on normal line printers or on graphic devices like plotters or graphic terminals.
Animals↗
Regulated expression of repetitive sequences including the identifier sequence during myotube formation in culture.
We have isolated and characterized a cDNA of 1183 bp, pL6-411, from rat L6 muscle cells. This cDNA contains repetitive sequences - including two inverted copies of the previously described identifier sequence - as shown by sequence analysis. Repetitive sequences from pL6-411 characterize a family of RNAs which is specifically induced during L6 myotube formation. Another part of the pL6-411 sequence, existing at low-copy number per haploid rat genome, hybridized to two RNAs of 5 kb and 2 kb from L6 myoblasts as well as from L6 myotubes. A third pL6-411-related RNA of 150 bases was detected which hybridized with the repetitive sequence but did not hybridize with the low-copy number part of pL6-411. It appears that the 'identifier' sequence in this population of small RNAs is complementary to one of the 'identifier' copies in the pL6-411-related RNA. Finally, we identified on cDNA pL6-411 the recognition site for the TGGCA-binding protein and in both orientations a total of four putative promoters for RNA polymerase III.
Animals↗
Visualization of nucleic acid sequence structural information.
Several interactive Pascal programs have been written for the analysis and display of structural information in nucleic acid sequences. Layout procedures were developed to display the homology and repeat matrices of a sequence and to predict and display the secondary structure of RNA/DNA molecules free of overlap and to predict and display internal repeats. No special plotting devices are required because the output is adapted to line printers. Sequences from several DNA database systems can be used as input. These programs are part of a general nucleic acid sequence analysis package.
Algorithms↗