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Biomedical subjects

K Sugimura

Publications and source records attributed to K Sugimura.

At least 253 records · Page 14Linked to original sources
PubMed1987

Serum creatine kinase isoenzymes in Duchenne muscular dystrophy determined by sensitive enzyme immunoassay methods.

Serum levels of creatine kinase (CK) isoenzymes (MM, MB, and BB) were measured by sensitive enzyme immunoassay (EIA) methods in 50 patients with Duchenne muscular dystrophy (DMD) and in 39 controls. MM, MB, and BB levels in DMD patients were higher than in controls, and these three levels decreased with advancing age of DMD patients. Serum MB levels showed a good correlation with serum levels of carbonic anhydrase III (CA-III), but correlated poorly with electrocardiographic findings, suggesting that the main origin of serum MB in DMD is skeletal muscle rather than cardiac muscle.

Adolescent↗
PubMed1987

Quantitative analysis by digital computer of 99mTc-N-pyridoxyl-5-methyltryptophan (99mTc-PMT) hepatogram in diffuse parenchymal liver diseases.

99mTc-N-pyridoxyl-5-methyltryptophan (99mTc-PMT) hepatograms were analyzed to provide information about the liver and bile duct. Calculations were based on a four compartmental model and included corrections for blood, tissue, hepatic parenchymal and bile backgrounds. The time activity curves for 99mTc-PMT in the cardiac region were described as the sum of two exponential functions, while curves for the hepatic regions were described as the sum of three exponential components. The measured hepatograms were compared with simulations and good agreement between the two curves showed that the compartmental model adequately described the blood and bile activities in vivo. Hepatic excretion rate constants were 0.179 +/- 0.026 in three normal subjects, 0.102 +/- 0.012 in four patients with chronic hepatitis and 0.082 +/- 0.034 in six patients with liver cirrhosis. In the case of diffuse parenchymal liver disease, there were lower rate constants for the excretion from the liver to the bile ducts than in normals, and the relative distribution volumes were also larger than normal. Prior to the development of this compartmental model, no useful kinetic model had been found which could satisfactorily explain the time activity curves. Experience in human studies proves this method to be accurate in determining the rate constants for the hepatobiliary transport of 99mTc-PMT.

Adult↗
PubMed1987

Bone marrow diseases of the spine: differentiation with T1 and T2 relaxation times in MR imaging.

Forty-two patients underwent magnetic resonance (MR) imaging for a variety of lesions in the vertebral body. A 0.15-T MR system was employed. Twenty-six patients were found to have malignant metastatic lesions (group 1); 16 had nonneoplastic lesions (group 2). The ability to differentiate between the two groups with MR imaging was evaluated. With the longer spin-echo repetition time, the image was variable in both groups. All malignant metastatic lesions appeared as low-intensity areas on T1-weighted images, but 50% of the nonneoplastic lesions also appeared this way. The mean T1 for group 1 was longer than that for group 2, but not significantly so. However, there were significant differences in the ratios of T1 to T2 and of the T1 ratio to the T2 ratio (T1 ratio = T1 for affected vertebrae/T1 for normal vertebrae, T2 ratio = T2 for affected vertebrae/T2 for normal vertebrae). These ratios were therefore useful in distinguishing malignant metastatic from nonneoplastic lesions.

Adolescent↗
PubMed1986

Effects of luteinizing hormone and dibutyryl adenosine 3',5'-monophosphate in cultured granulosa cells from polycystic ovaries.

The present study was undertaken to assess the ability of granulosa cells from subjects with normal and polycystic ovaries (PCO) to secrete progesterone throughout a 10-day culture period. LH levels in serum and follicular fluid from PCO patients were significantly (P less than 0.001) higher than those in normal subjects. In the absence of LH, progesterone secretion by granulosa cells cultured from PCO follicles did not differ significantly from that of cells from normal early and midfollicular phase follicles. Granulosa cells cultured from follicles from normal subjects in the early and midfollicular phases responded to LH (100 ng/ml) with an 8- to 20-fold increase in progesterone production. In contrast, LH increased progesterone production to a much lesser extent (up to 4-fold) in cells from the ovaries of patients with PCO. Progesterone secretion by granulosa cells from normal ovaries in response to LH diminished as intrafollicular endogenous progesterone and LH levels increased. Cells from PCO follicles cultured with (Bu)2cAMP (100 micrograms/ml) secreted progesterone in quantities comparable to those secreted by (Bu)2cAMP-stimulated normal ovaries in the early and midfollicular phases. These data demonstrate the discrepancy between the ability of granulosa cells from PCO and normal follicles to secrete progesterone in response to stimulation by LH and (Bu)2cAMP. These results suggest that in women with PCO, the persistent elevation of follicular LH may lead to impaired progesterone production in response to exogenous LH.

Adult↗
PubMed1986

A family with beta-galactosidase deficiency: three adults with atypical clinical patterns.

Three adult patients in a single family showed severe myoclonus, ataxia, and pyramidal signs. Enzymatic analysis of lymphocytes, plasma, and cultured skin fibroblasts showed marked deficiency of beta-galactosidase activity, more profound with GM1 ganglioside than with another natural substrate, asialofetuin. Other lysosomal hydrolases were normal. Although the physical signs were similar to those of types 1 and 2 GM1 gangliosidosis, none had bony abnormalities.

Adult↗
PubMed1985

Antigen-specific T cell suppressor factor (TsF): isolation of a cDNA clone encoding for a functional polypeptide chain of phosphorylcholine-specific TsF.

A cDNA library of phosphorylcholine (PC)-specific suppressor T hybridoma, 29-C-6, was constructed. By differential colony hybridization, 68 colonies were selected which hybridized with mRNA probes of two PC-specific suppressor T hybridomas, 29-C-6 and 09-M-24-8, but not with those of BW5147. A hybridization translation assay revealed that only one combination of translation products of cDNA clones, p6-1 and p6-5, showed strong PC-T cell suppressor factor (TsF) activity. Sequence analysis showed that p6-5 contained a specific cDNA sequence of about 800 base pairs (bp) while p6-1 had a 190 bp poly(A) sequence insert. When total poly(A) + RNA of 29-C-6 was hybridized with p6-1 filter alone the recovered mRNA was capable of producing PC-TsF. However, when the total poly-(A) + RNA was hybridized with a p6-1 filter combined with a p6-5 filter, the mRNA eluted from the p6-1 filter was not able to produce PC-TsF, suggesting that the depletion of p6-5 specific mRNA from p6-1-binding poly(A) + RNA led to a complete abolishment of the capability to produce PC-TsF. Furthermore, p6-5 hybridizing mRNA could successfully restore the p6-1-binding poly(A) + RNA depleted of p6-5-specific mRNA, and translation products of both RNA mixtures showed strong PC-TsF activity. These results suggest that PC-TsF is not a single polypeptide chain, but is composed of at least two distinct polypeptide chains, and also that p6-5 contains a cDNA sequence encoding for one of the polypeptide chains composing the PC-TsF molecule.

Animals↗
PubMed1985

Immunohistochemical demonstration of carbonic anhydrase III and muscle-specific enolase in paraffin-embedded human skeletal muscle sections.

We have demonstrated the histochemical fiber types of human skeletal muscle in paraffin sections by the immunohistochemical method, together with compatible observations previously made in frozen sections that carbonic anhydrase III is mainly localized in type 1 fibers (Shima et al. 1983), and muscle-specific enolase in type 2 fibers (Ibi et al. 1983). This method is useful to analyze the fiber types when frozen muscle samples at biopsy or autopsy cannot be obtained.

Animals↗
PubMed1985

Chronic experimental allergic neuritis (EAN) in juvenile guinea pigs: immunological comparison with acute EAN in adult guinea pigs.

In order to approach the mechanism of chronic or relapsing course in human chronic inflammatory demyelinating polyradiculoneuropathy, we established a chronic model of experimental allergic neuritis (EAN) in juvenile guinea pigs, and investigated the underlying cellular immune phenomenon in comparison with acute EAN in adult animals of the same strain. Two-week-old Hartley guinea pigs, sensitized with bovine peripheral nerve homogenate, developed chronic or relapsing EAN, whereas all adult animals developed acute monophasic EAN. Morphological examination of both the chronic and acute forms revealed scattered demyelination and mononuclear cell infiltrates which were essentially restricted to the peripheral nervous system, and indistinguishable from each other. Both the in vitro lymphocyte mitogenic response and in vivo skin testing revealed a significantly lower response to neuritogenic antigens (P2 protein and peripheral nerve myelin) in juvenile chronic EAN than in adult acute EAN throughout their respective courses. In addition, we showed, by means of assessing peripheral blood lymphocyte number and its subpopulations, that normal 2-week-old Hartley guinea pigs have not fully developed immunologically. These observations suggested that there was some immunological incompetence especially in cellular immunity in 2-week-old juvenile guinea pigs and that this might be one possible factor leading to chronic EAN.

Acute Disease↗