Biomedical subjects

K Suresh

Publications and source records attributed to K Suresh.

At least 19 recordsLinked to original sources

PubMed2006

Kocuria himachalensis sp. nov., an actinobacterium isolated from the Indian Himalayas.

A reddish orange bacterium, strain K07-05(T), was isolated from soil during a study of the bacterial diversity of a cold desert of the Indian Himalayas and was studied by using a polyphasic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Kocuria. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain K07-05(T) was closely related to Kocuria rosea DSM 20447(T) and Kocuria polaris MTCC 3702(T) (98.1 and 97.8 % sequence similarity, respectively), whereas the sequence similarity values with respect to the other Kocuria species with validly published names were between 96.4 and 94.2 %. However, the genomic relatedness, as shown by DNA-DNA hybridization, of strain K07-05(T) and K. polaris MTCC 3702(T) is 49.5 % and that with K. rosea MTCC 2522(T) is 24.0 %. The DNA G+C content of the strain is 75.3 mol%. The above data in combination with the phenotypic distinctiveness of K07-05(T) clearly indicate that the strain represents a novel species, for which the name Kocuria himachalensis sp. nov. is proposed. The type strain is K07-05(T) (=MTCC 7020(T)=DSM 44905(T)=JCM 13326(T)).

Actinobacteria↗

PubMed2006

Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes.

Four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. The four strains, 24K(T), 28K(T), 41KF2a(T) and 41KF2b(T), were identified as members of the genus Bacillus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that three of the strains, 24K(T), 28K(T) and 41KF2a(T), are very similar to one another (>98 % sequence similarity) and show a similarity of 98-99 % with Bacillus licheniformis and 98 % with Bacillus sonorensis. DNA-DNA hybridization studies showed that strains 24K(T), 28K(T) and 41KF2a(T) exhibit <70 % similarity with each other and with B. licheniformis and B. sonorensis. Differences in phenotypic and chemotaxonomic characteristics between the novel strains and B. licheniformis and B. sonorensis further confirmed that these three isolates are representatives of three separate novel species. Strain 41KF2b(T) showed 100 % 16S rRNA gene sequence similarity to Bacillus pumilus, but differed from its nearest phylogenetic neighbour in a number of phenotypic and chemotaxonomic characteristics and showed only 55 % DNA-DNA relatedness. Therefore, the four isolates represent four novel species for which the names Bacillus aerius sp. nov. (type strain, 24K(T)=MTCC 7303(T)=JCM 13348(T)), Bacillus aerophilus sp. nov. (type strain, 28K(T)=MTCC 7304(T)=JCM 13347(T)), Bacillus stratosphericus sp. nov. (type strain, 41KF2a(T)=MTCC 7305(T)=JCM 13349(T)) and Bacillus altitudinis sp. nov. (type strain, 41KF2b(T)=MTCC 7306(T)=JCM 13350(T)) are proposed.

Air Microbiology↗

PubMed2006

Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas.

A buff-yellow-pigmented bacterium, strain K22-20(T), which was isolated from a cold desert of the Indian Himalayas, was subjected to a polyphasic taxonomic study. Phenotypic and chemical properties of strain K22-20(T) were consistent with its classification in the genus Ornithinimicrobium. The major fatty acids of the strain were iso-C(17 : 1)omega9c (cis-15-methyl 7-hexadecenoic acid), iso-C(15 : 0) (13-methyl tetradecanoic acid), iso-C(16 : 0) (14-methyl pentadecanoic acid) and iso-C(17 : 0) (15-methyl hexadecanoic acid). The G+C content of the genomic DNA was 71 mol%. According to 16S rRNA gene sequence analysis, strain K22-20(T) was closely related to Ornithinimicrobium humiphilum HKI 0124(T) (97.7 %). However, genomic relatedness between strain K22-20(T) and O. humiphilum MTCC 6406(T), as revealed by DNA-DNA hybridization, was 64.5 %. Based on the polyphasic data, strain K22-20(T) (=MTCC 6545(T)=DSM 17687(T)=JCM 12763(T)) represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium kibberense sp. nov. is proposed.

Actinomycetales↗

PubMed2006

Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family "Flexibacteraceae".

A Gram-negative bacterial isolate (designated SRC-1(T)) was isolated from an occasional drainage system and characterized by a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SRC-1(T) with the family "Flexibacteraceae" of the phylum Bacteroidetes. It showed greatest sequence similarity to Pontibacter actiniarum KMM 6156(T) (95.5 %) followed by Adhaeribacter aquaticus MBRG1.5(T) (89.0 %) and Hymenobacter roseosalivarius DSM 11622(T) (88.9 %), but it differed from these micro-organisms in many phenotypic characteristics. Strain SRC-1(T) was an obligate aerobe and its cells were non-motile, irregular rods. The major fatty acids included mainly unsaturated and hydroxy fatty acids, including 17 : 1 iso I/anteiso B (36.7 %), 15 : 0 iso (15.8 %) and 17 : 0 iso 3-OH (10.3 %), and the DNA G+C content was 59.5 mol%. From the phenotypic and genotypic analyses it was clear that strain SRC-1(T) was quite different from members other genera in the family '"Flexibacteraceae". Therefore we conclude that strain SRC-1(T) represents a novel genus, for which the name Effluviibacter gen. nov., containing a single species Effluviibacter roseus sp. nov., is proposed. The type species of the genus is Effluviibacter roseus, the type strain of which is strain SRC-1(T) (=MTCC 7260(T)=DSM 17521(T)).

Aerobiosis↗

PubMed2006

Dietzia kunjamensis sp. nov., isolated from the Indian Himalayas.

A coral-red-pigmented actinobacterium, strain K30-10(T), was isolated from a soil sample from a cold desert of the Indian Himalayas. Chemical and phenotypic properties of strain K30-10(T) were consistent with its classification in the genus Dietzia. It showed 97.9 % 16S rRNA gene sequence similarity to Dietzia maris MTCC 7011(T); similarities to the type strains of three other species of the genus, Dietzia natronolimnaea, Dietzia psychralcaliphila and Dietzia cinnamea, were 94.4-96.0 %. The DNA-DNA relatedness between K30-10(T) and the closely related strain D. maris MTCC 7011(T) was 59.2 %. The DNA G+C content of strain K30-10(T) was 67.0 mol%. Based on physiological and biochemical tests and genotypic differences between strain K30-10(T) and its closest phylogenetic relatives, it is proposed that this strain represents a novel species, Dietzia kunjamensis sp. nov.; the type strain is K30-10(T) (=MTCC 7007(T)=DSM 44907(T)=JCM 13325(T)).

Actinomycetales↗

PubMed2006

Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas.

The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain K22-21(T), isolated from a soil sample from Lahaul-Spiti Valley in the Indian Himalayas, was determined using a polyphasic approach. The strain had phenotypic and chemical properties that were consistent with its classification in the genus Agrococcus. Alignment of the 16S rRNA gene sequence of strain K22-21(T) with sequences from Agrococcus jenensis DSM 9580(T), Agrococcus baldri DSM 14215(T) and Agrococcus citreus DSM 12453(T) revealed similarities of 98.5, 96.8 and 96.6 %, respectively. However, the level of DNA-DNA relatedness between strain K22-21(T) and A. jenensis was 55.1 %. The novel strain could be distinguished from type strains of the three species of the genus Agrococcus using DNA-DNA relatedness and phenotypic data. Based on these differences, strain K22-21(T) (=MTCC 7154(T)=DSM 17612(T)) should be classified as the type strain of a novel species of Agrococcus, for which the name Agrococcus lahaulensis sp. nov. is proposed.

Actinomycetales↗

PubMed2005

Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India.

The taxonomic position of a bacterium isolated from a cold desert of the Himalayas, India, was analysed by using a polyphasic approach. The isolated strain, designated K22-03T, had phenotypic characteristics that matched those of the genus Planococcus and it represents a novel species. The almost-complete 16S rRNA gene sequence (1464 bases) of the novel strain was compared with those of previously studied Planococcus type strains and confirmed that the strain belongs to the genus Planococcus. 16S rRNA gene sequence analysis indicated that strain K22-03T differs from all other species of Planococcus by at least 2.5 %. DNA-DNA hybridization showed that it had low genomic relatedness with Planomicrobium mcmeekinii (MTCC 3704T, 23 %), Planococcus psychrophilus (MTCC 3812T, 61 %), Planococcus antarcticus (MTCC 3854T, 45 %) and Planomicrobium okeanokoites (MTCC 3703T, 51 %), the four species with which it was most closely related based on 16S rRNA gene sequence analysis (97-97.5 % similarity). Therefore, strain K22-03T should be recognized as a novel species, for which the name Planococcus stackebrandtii sp. nov. is proposed. The type strain is K22-03T (=MTCC 6226T=DSM 16419T=JCM 12481T).

Bacterial Typing Techniques↗

PubMed2005

Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica.

A Gram-negative, rod-shaped, psychrophilic, motile, non-spore-forming bacterium, strain U1T, was isolated from Ushuaia located at the southernmost tip of Argentina. On the basis of 16S rRNA gene sequence similarity, strain U1T was found to be closely related to Marinomonas communis (DSM 5604T) and Marinomonas primoryensis (IAM 15010T). At the DNA-DNA level, however, the values for similarity were 41 and 25 %, respectively. The major fatty acids present were iso-C(16 : 0), C(16 : 1)omega7c, iso-C(17 : 1) and C(18 : 1)omega7c and the G+C content of the DNA was 43.6 mol%. All of the above characteristics support the affiliation of strain U1T to the genus Marinomonas. Furthermore, on the basis of phenotypic features, chemotaxonomic characteristics and phylogenetic analysis of the 16S rRNA gene sequence, it appears that strain U1T is distinct from the four Marinomonas species with validly published names. Strain U1T, therefore, represents a novel species, for which the name Marinomonas ushuaiensis sp. nov. is proposed. The type strain of M. ushuaiensis is U1T (=MTCC 6143T=DSM 15871T=JCM 12170T).

Antarctic Regions↗

PubMed2005

Bacillus arsenicus sp. nov., an arsenic-resistant bacterium isolated from a siderite concretion in West Bengal, India.

Strain Con a/3(T) is a Gram-positive, motile, endospore-forming, rod-shaped and arsenic-resistant bacterium, which was isolated from a concretion of arsenic ore obtained from a bore-hole. The bacterium grew in the presence of 20 mM arsenate and 0.5 mM arsenite. Diaminopimelic acid was present in the cell wall peptidoglycan, MK-7 was the major menaquinone, and iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0) and C(16 : 1)(delta7cis) were the major fatty acids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain Con a/3(T) was identified as a member of the genus Bacillus. It exhibited maximum similarity (97 %) at the 16S rRNA gene level with Bacillus barbaricus (DSM 14730(T)); however, the DNA-DNA relatedness value with B. barbaricus was 60 %. Strain Con a/3(T) also exhibited a number of phenotypic differences from B. barbaricus (DSM 14730(T)). Strain Con a/3(T) was therefore identified as representing a novel species of the genus Bacillus, for which the name Bacillus arsenicus sp. nov. is proposed. The type strain is Con a/3(T) (= MTCC 4380(T) = DSM 15822(T) = JCM 12167(T)).

Anti-Bacterial Agents↗

PubMed2005

Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands.

A psychrotolerant, Gram-negative, motile bacterium, designated CK 47(T), was isolated from sea water off the subantarctic Kerguelen islands (50 degrees 40' S 68 degrees 25' E). The isolate grew optimally at 22 degrees C and minimum and maximum temperature of growth were 4 and 37 degrees C, respectively. It required Na(+) for growth and exhibited optimum growth at pH 8.5 and 4% NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47(T) had Q9 as the major respiratory quinone and C(16 : 0) (21.7%), C(17:0) (21.3%), C(18:0) (5.7%), C(18:1)omega7c (9.0%) and C(18:1)omega9c (31.4%) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47(T) formed a coherent cluster within the genus Marinobacter. It exhibited highest 16S rRNA gene sequence similarity of 96.8% with Marinobacter lipolyticus. However, the level of DNA-DNA relatedness between strain CK47(T) and M. lipolyticus was only 55%. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47(T) is considered to represent a novel species of the genus Marinobacter. The name Marinobacter maritimus sp. nov. is proposed, with CK 47(T) (=JCM 12521(T)=MTCC 6519(T)) as the type strain.

Alteromonadaceae↗

PubMed2005

Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India.

Strain HHS 22(T) was isolated from a glacial water sample from the snout of the Hamta glacier located in the Himalayan mountain ranges of India. Phenotypic, chemotaxonomic and phylogenetic analyses established the affiliation of the isolate to the genus Pedobacter. HHS 22(T) exhibits high 16S rRNA gene sequence similarity with Pedobacter cryoconitis (98 %). However, the level of DNA-DNA relatedness between HHS 22(T) and P. cryoconitis is only 42 %. Furthermore, HHS 22(T) differs from P. cryoconitis and the four other recognized species of Pedobacter in a number of phenotypic characteristics. These data suggest that HHS 22(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter himalayensis sp. nov. is proposed. The type strain is HHS 22(T) (= JCM 12171(T) = MTCC 6384(T)).

Bacterial Typing Techniques↗

PubMed2005

Viable blastocystis cysts in Scottish and Malaysian sewage samples.

Blastocystis cysts were detected in 38% (47/123) (37 Scottish, 17 Malaysian) of sewage treatment works. Fifty percent of influents (29% Scottish, 76% Malaysian) and 28% of effluents (9% Scottish, 60% Malaysian) contained viable cysts. Viable cysts, discharged in effluent, provide further evidence for the potential for waterborne transmission of Blastocystis.

Animals↗

PubMed2005

Lipid peroxidation & antioxidants status in patients with oral squamous cell carcinoma.

BACKGROUND & OBJECTIVE: Overproduction of lipid peroxidation byproducts and disturbances in antioxidant defense system have been implicated in the pathogenesis of several diseases including oral cancer. Though several studies have been done on the level of lipid peroxidation and antioxidants in oral cancer patients, there are no reports in patients with various clinical stages of oral squamous cell carcinoma. We carried out this study to assess the level of oxidative stress in oral cancer patients with various clinical stages. METHODS: Blood samples of 48 adult male oral cancer patients with various clinical stages of oral cancer (stage II to stage IV, 16 of each) and 16 age and sex matched healthy subjects were collected. Plasma and erythrocytes levels of thiobarbituric acid reactive substances (TBARS), vitamin E, reduced glutathione (GSH), and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were assayed using specific colorimetric methods. The statistical comparisons were performed by one way analysis of variance (ANOVA) followed by Student's t-test. RESULTS: Elevated lipid peroxidation and decline in non-enzymatic and enzymatic antioxidants status were noticed in oral cancer patients as compared to healthy subjects. The TBARS levels were gradually increased whereas antioxidants were gradually reduced from stage II to stage IV of oral cancer patients. INTERPRETATION & CONCLUSION: The altered lipid peroxidation in plasma and erythrocytes of oral cancer patients may be related to their compensatory changes in the antioxidants defense system.

Adult↗

PubMed2005

Study of bacterial, fungal, and parasitic contaminaiton of currency notes in circulation.

A total of 100 currency notes of various denominations in circulation were randomly studied for bacterial, fungal and protozoal contamination. All except four notes yielded one or more bacteria. Bacterial culture yielded single isolate in 33 notes, two in 44 notes, three in 12 notes and four in 7 notes. The predominant bacterial isolate was Bacillus sps followed by Coagulase negative Staphylococci and Micrococcus sps. Other bacteria that are either potential or confirmed pathogens included K. pneumoniae, E. coli, S. aureus, Pseudomonas sps and S. typhi. Only two notes were positive for Acid fast bacilli. 28 samples did not yield any fungal growth. Overall 118 fungal isolates were isolated, of which 34 could not be identified. All the fungi isolated were saprophytes. Saline and Iodine wet mount did not reveal any parasitic forms. We recommend that currency notes must be handled with caution.

Animals↗

PubMed2004

Comparison of methods for detecting Blastocystis hominis.

In order to determine the comparative sensitivity of two methods of detecting Blastocystis hominis and to investigate the seasonality of infection with this enteric protozoan parasite, the present study was conducted. In each of two 3-month periods representing winter, spring (February-April) and summer (July-September), 500 routine stool submissions were examined for B. hominis using microscopy following either formol-ether concentration or in vitro culture using Jones' medium. The organism was detected in 39 of the 1,000 samples investigated using the in vitro culture technique and in none of the samples using the formol-ether concentration technique. In 82% of the B. hominis-positive samples, no concurrent bacterial or parasitic pathogens were found, and diarrhoea was the most commonly recorded symptom among patients. Infection was more prevalent in summer than in winter/spring, occurring primarily in the 71-80-year age group. Cysts were detected in 20.5% of positive samples, but only following Ficoll-Paque concentration of formol-ether concentrates. Cyst excretion was more prevalent in summer than in winter/spring.

Adolescent↗

PubMed2004

Analysis of glycoconjugates in patients with oral squamous cell carcinoma.

BACKGROUND: Our aim was to examine the levels of glycoconjugates in plasma, erythrocyte membranes and buccal mucosa of healthy subjects and oral cancer patients. SUBJECTS AND METHODS: This study was conducted on 48 adult male oral cancer patients with various clinical stages (stage II to stage IV; 16 of each) and 16 disease-free healthy subjects who underwent surgical removal of impacted teeth or vestibuloplasty without inflammation. RESULTS: The plasma and tumor tissues glycoconjugates levels were significantly increased, whereas the erythrocyte membranes glycoconjugates were significantly decreased in oral cancer patients as compared to healthy subjects. The levels of glycoconjugates were gradually increased from stage II to stage IV in plasma and tumor tissues and decreased in erythrocyte membranes from stage II to stage IV of oral cancer patients. CONCLUSION: The increased plasma glycoconjugates can be due to the expense of erythrocyte membrane glycoconjugates or tumor tissue itself.

Adult↗

PubMed2004

Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India.

An arsenic- and radiation-resistant bacterium, strain Wt/1a(T), was isolated from water from an arsenic-contaminated aquifer located in the Chakdah district of West Bengal, India. The bacterium stains Gram-negative and is rod-shaped, non-motile, non-sporulating and red-pigmented. Cell-wall peptidoglycan contains ornithine as the diamino acid, MK-8 is the major menaquinone, C(15 : 1) and C(16 : 1) are the major fatty acids and the DNA G+C content of the organism is 65.8 mol%. Based on these phenotypic and chemotaxonomic characteristics, strain Wt/1a(T) was identified as a member of the genus Deinococcus. Strain Wt/1a(T) exhibited maximum 16S rRNA gene sequence similarity (95 %) with Deinococcus grandis; however, strain Wt/1a(T) exhibited only 14 % similarity to D. grandis IAM 13005(T) at the DNA-DNA level. Furthermore, strain Wt/1a(T) (compared to D. grandis IAM 13005(T)) is more resistant to arsenate and arsenite, is positive for arginine dihydrolase, utilizes a number of carbon sources and exhibits quantitative differences in fatty acid composition and qualitative differences in lipid composition. Strain Wt/1a(T) is identified as a novel species of the genus Deinococcus, for which the name Deinococcus indicus sp. nov. is proposed. The type strain of Deinococcus indicus is Wt/1a(T) (=MTCC 4913(T)=DSM 15307(T)).

Arsenic↗

PubMed2004

Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India.

Strain Sd/3T (=MTCC 4374T=DSM 15820T), an arsenic-resistant bacterium, was isolated from a sand sample obtained from an arsenic-contaminated aquifer in Chakdah district in West Bengal, India (23 degrees 3' N 88 degrees 35' E). The bacterium was Gram-positive, rod-shaped, non-motile, endospore-forming and yellowish-orange pigmented. It possessed all the characteristics that conform to the genus Bacillus, such as it had A4beta murein type (L-orn-D-Asp) peptidoglycan variant, MK-7 as the major menaquinone and iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. Based on its chemotaxonomic and phylogenetic characteristics, strain Sd/3T was identified as a species of the genus Bacillus. It exhibited maximum similarity (95%) at the 16S rRNA gene level with Bacillus cohnii; however, DNA-DNA similarity with B. cohnii was 60.7%. Strain Sd/3T also exhibited a number of phenotypic differences from B. cohnii (DSM 6307T). These data suggest that Sd/3T represents a novel species of the genus Bacillus. The name Bacillus indicus sp. nov. is proposed.

Anti-Bacterial Agents↗