Isolation and nucleotide sequence of cDNA clone encoding nodule-specific (hydroxy)proline-rich protein LENOD2 from yellow lupin.
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Amino Acid Sequence↗
Biomedical subjects
K Szczygłowski
Publications and source records attributed to K Szczygłowski.
Glutamine synthetase in Lupinus luteus. Identification and preliminary characterization of nodule-specific cDNA clone.
Two glutamine synthetase (GS) cDNA clones from L. luteus were identified and characterized. The nucleotide sequence analysis proved that they represent highly homologous but distinct mRNA species. Northern blot hybridization revealed that pc LINGS encodes the nodule-specific subunit of the GS while pcLIGS1 represents the nonspecific one present in nodule tissue as well as in uninfected roots.
Amino Acid Sequence↗
Cloning and identification of lupin nodule specific cDNA sequences.
Yellow lupin nodule specific sequences were selected by screening of cDNA library prepared from lupin nodule poly(A)+RNA. From about 3,000 clones containing fragments of lupin DNA 150-1,500 base pair long, 7% of clones carrying nodule specific sequences were identified. Among them the most abundant sequence species, represented by 32% clones, encodes leghemoglobin. Another abundant species designated pLN13 is represented by 13% clones. The Northern blot analysis of lupin mRNA confirmed nodule specificity of the cloned sequences. The nucleotide sequence of one clone, pLN281 of 225 bp, is presented.
Base Sequence↗