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Biomedical subjects

K T Rong

Publications and source records attributed to K T Rong.

7 recordsLinked to original sources
PubMed1995

An explanation on the limited efficacy of detoxication against VX toxicity by purified specific antibodies.

Studies on the mechanism of detoxication against VX toxicity by purified rabbit anti-VX antibodies were carried out in mice. VX (25 micrograms/kg) was injected subcutaneously immediately following intravenous injection of purified anti-VX antibodies. Blood concentration of free VX declined linearly as the antibody dose increased. Free VX concentration in brain was far lower than that in blood. When the dose of purified anti-VX antibodies was 8 and 6 mg/kg, respectively, free VX concentration in brain and blood approached 0. At the same time, the cholinesterase (ChE) activity in brain increased along with increasing antibody dose, with 70% of normal control value detected when 8 mg/kg antibodies were administered. On the other hand, anti-VX antibodies were not able to protect blood ChE unless the VX dose was lower than 10 micrograms/kg. The studies suggest that the protective action of purified rabbit anti-VX antibodies is from VX antibodies combining with the free VX in blood and extravascular tissue to reduce the amount of VX entering into the brain.

Animals↗
PubMed1995

Production, characterization and application of monoclonal antibodies against the organophosphorus nerve agent Vx.

Two monoclonal antibodies (Vx-BB8 and Vx-EA11) to the chemical warfare agent Vx were produced and characterized. A competitive inhibition enzyme immunoassay was developed to detect Vx concentrations as low as 3.7 x 10(-7) - 3.7 x 10(-6) mol/l in biological samples. Vx-BB8 400 micrograms given intravenously immediately before 1 x LD95 Vx or 400 micrograms Vx-BB8 intraperitoneally 1.5 h-3 days before 1 x LD95 Vx could protect all the tested mice from death.

Animals↗
PubMed1995

Detection of the organophosphorus nerve agent sarin by a competitive inhibition enzyme immunoassay.

Two artificial antigens, NalphaNepsilon-di(O,O-diisopropyl) phosphoryl L-lysine (DIP)- bovine serum albumin (BSA) conjugate (DIP-BSA) and DIP-KLH (keyhole limpet hemocyanin), were synthesized. Antibodies against sarin (O-isopropyl methylphosphonofluoridate) were obtained after immunization of rabbits with DIP-KLH conjugate. A competitive inhibition enzyme immunoassay (CIEIA) was developed to detect the organophosphorus nerve agent sarin. The antibody solutions could be inhibited by sarin as low as 10(-6) mol/l, and the standard curve was linear over 3 orders of magnitude. The coefficients of intraassay and interassay variation of this method were 5.4-6.2% (n = 11) and 8.0-9.5% (n = 6) at a sarin concentration range of 10(-3)-10(-6) mol/l, respectively. The recovery of sarin in water samples at the concentration of 5 x 10(-5) mol/l was in the range of 96.8-102.5%. The specificity of the antiserum was assessed by comparing the inhibition induced by sarin with soman, Vx, isopropyl alcohol and isopropyl methyl phosphonic acid. The results showed that less than 5 mmol/l soman, 2 mmol/l Vx, 16 mmol/l isopropyl alcohol and 8 mmol/l isopropyl methyl phosphonic acid did not influence the determination of sarin in water samples.

Animals↗
PubMed1990

Immunologic protection against VX intoxication in experimental animals.

Rabbits immunized with an artificial VX-antigen could survive 1 x LD95 of VX challenge on the 7th day and the 31st day after the last immunization. One hundred microliters of rabbit anti-VX antiserum given intravenously immediately before 1 x LD95 of VX or 200 microliters of antiserum intraperitoneally 1-10 days before 1 x LD95 VX protected all the tested mice from death. The antiserum could prevent the in vitro inhibition of Torpedo AChE activity by VX and reduced its effect on brain AChE activity in vivo. No protective effect of the antiserum was observed on the Torpedo AChE activity inhibited by sarin and soman.

Acetylcholinesterase↗