[Studies on the influence of potassium concentration on the interaction between actomyosin and ATP].
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Biomedical subjects
Publications and source records attributed to K TAKEYA.
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The process of multiplication of mycobacteriophage B-1 in its host cell was studied by means of an improved technic of ultrathin sectioning. The appearance of the nuclear apparatus was not altered throughout the latent period. Phage-shaped dense particles appeared about 30 minutes after infection in less dense areas neighboring the nuclear apparatus and occasionally at the margin of the nuclear apparatus. The less dense areas, which may correspond to the phage multiplication foci according to the authors' interpretation, were not filled with such arrays of fine-stranded fibrils as are seen in the nuclear apparatus. Empty phage heads could frequently be seen within and outside the lysed cells, along with the mature phage particles, at the end of the latent period. Moreover, it was indicated that empty head membranes may possibly exist within the cells during the latent period
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The fine structure of the intracytoplasmic organelles of mycobacteria was studied by means of electron microscopy of ultrathin sections. A well-preserved nuclear apparatus was obtained by fixation with OsO(4) in acetate-veronal buffer, containing calcium and tryptone, or in collidine-HCl buffer, followed by uranyl-acetate treatment and embedding in araldite. A low density nuclear region was filled with fine fibrils, 30 A in diameter, in parallel or concentric arrangement. A membranous organelle, tentatively designated as "lamellar structure," consists of unit membranes in lamellar arrangement. The thickness of each lamella in this membranous organelle coincides with that of the three-layered cytoplasmic membrane Moreover, the continuity of this unit membrane with the cytoplasmic membrane was demonstrated.
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