Biomedical subjects

K Tanioku

Publications and source records attributed to K Tanioku.

At least 19 recordsLinked to original sources

PubMed1979

The distribution of 2,4-dinitrophenyl groups in lymphoid tissue of guinea-pigs following skin painting with 2,4-dinitrochlorobenzene.

Cellular localization of 2,4-dinitrophenyl (DNP) groups in the peripheral lymphoid system of guinea-pigs was studied at various times after painting the skin with 2,4-dinitrochlorobenzene (DNCB) by the immunofluorescent method using anti-DNP antibody. The cells taking up the stain (DNP cells) were shown to be mainly lymphocytes. At 1-6 h after painting the majority of DNP cells were found in the peripheral blood and the spleen but the maximum number was reached in the lymph node draining the site of DNCB application 12 h after painting. Injecting cyclophosphamide (CY) 3 days before painting with DNCB, heightened the number of DNP cells residing in the draining node. The animals treated with the tolerogen, 2,4-dinitrobenzene sulphonic acid sodium salt (DNBSO3Na), and then painted with DNCB, had fewer DNP cells than those animals which had simply been painted once with DNCB. The culture supernatants prepared from the draining nodes of both normal and tolerant animals partially blocked the anti-DNP antibody binding with DNP cells. It is suggested that the cells associated with DNP groups residing in the draining lymph node act as immunogens in the immunizing process of contact sensitivity.

Administration, Topical↗

PubMed1978

Studies on the retest reaction in contact sensitivity to DNCB.

DNCB-sensitized guinea pigs demonstrated an accelerated reactivity on retest of DNCB at the site of prior contact reaction, though presenting normal contact sensitivity at the virgin site. The retest reaction reached maximal at 9 h and waned at 24 h after antigenic challenge. Massive accumulation of eosinophils in either the epidermis or dermis was its distinguishing histologic feature. The reaction was induced at the site of delayed skin reaction to DNP-GPE in the animals sensitized with DNCB or DNP-GPE. A retest reaction in delayed sensitivity to DNP-GPE was also elicited at the site of contact reaction to DNCB in the animals. The significance of these findings is discussed.

Animals↗

PubMed1978

Induction of delayed reaction to dinitrophenylated lymphocytes in guinea pigs with contact sensitivity to DNCB.

The incidence of dinitrophenylated cells in guinea pig lymphocytes incubated with 0-30 mM concentrations of DNBSO3Na in phosphate-buffered saline was examined by an immunofluorescence method using fluorescence-labelled anti-DNP antibody. Under our experimental conditions, the incidence was roughly proportional to the concentration used. Using DNP-lymphocytes as an antigen for skin testing, a marked delayed reaction was induced in guinea pigs sensitized by painting with DNCB and intradermal injection of Freund's complete adjuvant. The significance of these findings is discussed.

Animals↗

PubMed1978

The distribution of antigen in flare up reaction in contact sensitivity to DNCB.

The distribution of DNP groups in various tissues of guinea-pigs following intravenous injection of DNBSO3Na was investigated by an immunofluorescent method using FITC-labelled anti-DNP antibody. DNP groups were detected either in the cytoplasms of epidermal cells or on/in lymphoid tissue cells. The epidermal distribution of DNP groups was shown in the skin obtained from 5 min to 3 days after injection. There was no fundamental difference in the epidermal localization of DNP groups between at the previously tested site and at virgin site in DNCB sensitized animals. Unreacted DNBSO3Na was demonstrated in the blood plasma of DNBSO3Na injected animals. The injection of either DNP-lysine or 2,4-dinitrophenol was found incapable of inducing flare up reaction and no epidermal localization of DNP groups in the sensitized animals was observed. A possibility that intravenously injected DNBSO3Na forms a complete antigen by conjugation with epidermal proteins and sensitized cells, which have been indicated by Polak, Turk & Frey (1973) to remain at the site of old contact reaction, react with the antigen resulting in flare up reaction, is suggested.

Animals↗

PubMed1977

Studies on the dinitrophenylated lymphocytes in guinea pig painted with DNCB.

The number of DNP group-bearing lymphocytes in the regional lymph node, thoracic duct and peripheral blood was determined at various time intervals after painting normal guinea pigs with DNCB by the immunofluorescent method using anti-DNP antibody. The incidence of such cells in the regional node was maximal at 12 hours whereas in the thoracic duct and peripheral blood the maximum incidence was found at 0.1-2 hours after painting. Unreacted DNCB was demonstrated in both the thoracic lymph duct and the blood at least for 12 or 24 hours respectively following exposure to DNCB. The authors therefore suggest that DNCB reacts directly in vivo with the lymphocyte cell membrane of guinea pig following epicutaneous application of the chemical.

Animals↗

PubMed1977

The effects of castration and testosterone administration on mast cells and hydroxyproline in rat skin.

In rat skin of both sexes, significantly higher concentrations of mast cells were observed in the genital area as compared with the skin of the back. The hydroxyproline (HPL) value in the preputial skin was half that in the dorsal skin. The influence of castration and the administration of testosterone on male rat skin mast cells and HPL were investigated. A possible relationship was pointed out between HPL value and the mast cell population of the skin.

Animals↗

PubMed1977