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Biomedical subjects

K Titlestad

Publications and source records attributed to K Titlestad.

8 recordsLinked to original sources

Monitoring transfusion practice--a computerized procedure.

As the description of transfusion practice over time requires the handling of massive amounts of data, and because the majority of records have until now been paper-based, little is known about the epidemiology of blood transfusion. Furthermore, most previous studies have collected data manually from patient records or from computerized registers created solely for study purposes. We describe a procedure for monitoring transfusion practices based exclusively upon the collection of data from the existing computerized registers. During the calendar years 1997 and 1998, more than 750 000 data records were collected from the blood transfusion registers, diagnosis and procedure registers, and clinical biochemistry registers at two university hospitals. Linking of the data required a unique and consistent identification of all data records. This was made possible by the national Personal Identification Number. In this way more than 98% of all transfusions could be identified and approximately 95% (77 950) could be connected to a principal diagnosis. The described procedure creates new opportunities for studies of transfusion practices both on a large scale and in richness of detail, and we propose to establish a network where transfusion practices data are available and comparable for individual departments, hospitals, whole regions, and possibly nations.

Blood Transfusion↗

Monitoring transfusion practices at two university hospitals.

BACKGROUND AND OBJECTIVES: Considering the clinical importance of blood transfusions, the limited knowledge of transfusion practices is remarkable. New methods are needed to elucidate the observed variation of transfusion practices. MATERIALS AND METHODS: All patients transfused or pretransfusion tested (i.e. at risk for blood transfusion) at two tertiary teaching hospitals during the full years of 1997 and 1998 were included in the study. RESULTS: The observed practices contained substantial variance at the level of hospitals. The contributions to the total variance of the different specialities were highly significant, as was the contribution of hospitals to the variance in plasma and platelet transfusions. Significant interactions between hospitals and specialities were observed in transfusion of red cells, plasma and platelets. CONCLUSION: To our knowledge this is the first survey of this size to combine transfusions, diagnoses and biochemical tests, exclusively based on an existing computerized register. The aim is to establish a basis for comparison of local transfusion practices with other commensurate hospitals and regions at a national (and international) level.

Adolescent↗

Detection of irregular red cell antibodies: more than 3 years of experience with a gel technique and pooled screening cells.

BACKGROUND AND OBJECTIVES: The purpose of this study was to evaluate more than 3 years of experience with a gel technique in combination with pooled screening cells for the detection of irregular red cell antibodies. MATERIALS AND METHODS: Conventional serologic methods were used for blood typing, antibody screening and cross-matching until the end of 1992. We introduced the gel technique as a routine assay for antibody detection and identification in 1993. RESULTS: After the tube technique had been abandoned, the number of false-positive antibody screening tests was reduced by 71%, positive antibody screening tests by 33%, enzyme agglutination by 100% and rouleaux reactions and cold-reacting antibodies by more than 50%. There was a 40% increase in first-time detection of clinically relevant antibodies. We saw no increase in delayed haemolytic transfusion reactions. CONCLUSIONS: For the detection of irregular red cell antibodies, pooled screening cells in combination with a gel technique are at least as efficient and safe as a conventional tube technique with unpooled test cells.

Blood Grouping and Crossmatching↗

A synthetic hexadecapeptide derived from allergen M imposing allergenic and antigenic reactivity.

A solid phase synthesis (SPPS) hexadecapeptide corresponding to residues 49-64 of allergen M from cod was found to bind specifically IgE antibodies from sera of cod-allergic individuals. The SPPS peptide was similarly reactive in IgG-mediated reactions; it could interfere with Allergen M line precipitate in rocket-line immunoelectrophoresis, giving a distinct deflection. It is the only reported synthetic polypeptide imposing allergenic and antigenic reactivity. The hexadecapeptide is composed of two homologous tetrapeptides located at its terminai, tentatively concluded to be mutually critical for the immunological specificity. The data obtained confirmed the predicted reactivity of the Ca2+-binding CD loop of the intact Allergen M.

Allergens↗

Patterns of peptides and protein-associated-peptide complexes in psychiatric disorders.

Peptidic neurones may be considered as multisignal intergrators and transducers. When formation or release of peptide outstrips genetically determined breakdown capacity, overflow of peptides to the body fluids and urine may be expected. In this paper, pathological urinary chromatographic patterns of peptides are shown for genetic, functional and mixed disorders. Part symptoms of the disorders may be induced with the biologically isolated and purified peptides as well as with chemically synthesized peptides.

Adolescent↗

Cyclic peptides of sarcosine. Syntheses and conformation.

A series of cyclic peptides of sarcosine with the general formula c-Sar-n, n=2-8, has been synthesized and conformational studies carried out both in solution and in the solid. The rings are conformationally very homogeneous and contain both cis and trans amide bonds. Their barriers to ring inversion are high; in the smaller rings this is attributed to steric hindrance, caused by the N-methyl-groups, whilst in the larger rings the folding of the chain in helical segments plays an important role.

Cyclization↗