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K Tozuka

Publications and source records attributed to K Tozuka.

49 records · Page 3Linked to original sources
PubMed1983

Study of passed stones by means of X-rays, infrared and thermal analyses.

X-ray powder diffraction, infrared spectroscopy and thermal analysis were used to study 108 stones that had been passed spontaneously from the upper urinary tract. The presence of apatite in pure calcium oxalate stones classified by x-ray diffraction was confirmed by infrared analysis of the ignited product. Consequently, 102 of 108 stones were found to be composed of weddellite and/or whewellite, and apatite. Besides the contents of weddellite, whewellite and apatite, the content of organic matrix also was estimated by thermal analysis in 102 stones, ranging from 1.1 to 17.2 per cent. The stones then were divided into 3 groups according to the principal constituent: 1) weddellite, 2) whewellite and 3) apatite. Weddellite stones were significantly heavier and significantly higher in apatite content than whewellite stones. It is suggested that weddellite calculi tend to become larger than whewellite stones. The apatite content was correlated with the ratio of weddellite to weddellite plus whewellite ( r equals 0.528), and the content of organic matrix was correlated with the whewellite content (r equals 0.708). Thus, it is considered that the weddellite-apatite and whewellite-organic matrix associations have important roles in the development and growth of weddellite and whewellite calculi, respectively.

Apatites↗
PubMed1983

[Studies on human prostatic carcinoma cell lines: evaluation of acid phosphatase productivity and effects of sex hormones on the cells].

The established cell lines from human prostatic cancer, such as Duke 145, 8PC93, and 19PC93, were examined in terms of their producing activity of acid phosphatase (ACP) and sensitivity to sex hormones. The results obtained are summarized. 1. ACP producing activity ACP was estimated with phenyl phosphate as a substrate. Values of the materials from each of the cells extracted with 5% Triton X-100 were Duke 145 (6.1 u/mg), 8PC93 (40.6 u/mg), and 19PC93 (40.4 u/mg), respectively. Activities of ACP were prohibited by the presence of L-tartrate. Histochemistry of ACP was demonstrated by azo-dye staining procedure, revealing the positive reactions in the cytoplasms of 8PC93 and 19PC93 cells, but weak reaction in duke 145 cells. Disk polyacrylamide gel electrophoresis (D-PAGE) was employed for ACP analysis of the cell extracts with 5% Tryton X-100 treatment. Two main bands were observed near original point and at another point proposed as ACP-2. These ACP positive reactions on the gels were also inhibited by the presence of L-tartrate in staining solution. In the case of Duke 145 cell material, the intensity of the reaction was observed weak in those specific two bands. 2. Hormone effects to the cells The prostatic cancer cells were examined in terms of sensitivity to sex steroid hormones such as androsterone, progesterone, estrone, estradiol, and estriol, by a colony formation method. Fifty percent reduction in colony formation of the 8PC93 and 19PC93 cells was found at the concentration of ca. 1.5 micrograms/ml in the case using progesterone or estrone, or estradiol, while 50% reduction of the Duke 145 cells was observed at 5 micrograms/ml only in a case using progesterone.

Acid Phosphatase↗
PubMed1981

Chemical test of phosphates in urinary stones by means of the chromatographic contact print method.

The chromatographic contact print method, making use of the ammonium phosphomolybdate-ascorbic acid reaction, is used to detect phosphates and to view their distribution in urinary stones. This sensitive reaction may take place even if phosphates, especially apatite, are present in such small amounts as to be under the confirmation limits of microscopic observation, and X-ray and infrared analyses.

Apatites↗