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Biomedical subjects

K Try

Publications and source records attributed to K Try.

At least 37 records · Page 2Linked to original sources
PubMed1987

Protein C: an automated activity assay.

Protein C in citrated plasma is found to be specifically activated by the snake venom derivative Protac, and the activator is used in a simple, automated assay method. The activation is completed in less than 120 s and an isolation of protein C from its inhibitor before activation is not necessary. The activated protein C is determined with the chromogenic substrate S-2366. Therapeutic concentrations of heparin in the test sample do not influence the result. A strong positive correlation to immunoassay of protein C was found (r = 0.92). Three cases of probable hereditary protein C deficiency belonging to the same family were discovered during the study.

Adult↗
PubMed1986

The Tromsø Heart Study: distribution of, and determinants for, gamma-glutamyltransferase in a free-living population.

Gamma-glutamyltransferase (GGT) was measured in 1579 men, aged 20-54 years, and 1654 women, aged 20-49 years, screened for coronary risk factors. The distribution was right-skewed with medians 15 and 10 U/l for men and women, respectively. Less than 3.8% of the men and 0.8% of the women had GGT greater than or equal to 50 U/l. The low level of GGT matched well with the low mortality of cirrhosis and the modest use of alcohol in Norway. Multiple regression analysis for each sex showed a strong positive association with body mass index, use of alcohol and, unexpectedly, a negative association with coffee consumption, whereas serum triglycerides and the time since the last meal showed a weaker positive association. In women, use of oral contraceptives was positively associated with GGT.

Adult↗
PubMed1982

High performance thin layer chromatography of disaturated phosphatidylcholines in amniotic fluid.

A method for isolation and quantification of disaturated phosphatidylcholines in amniotic fluid is described. Phospholipids are extracted with chloroform/methanol, and the unsaturated lipids are reacted with osmium tetroxide. The saturated phospholipids are separated on high performance thin layer silica gel plates with a concentrating zone. The coloured lipid spots are quantified by reflectometric scanning and compared with standards. The between-day coefficient of variation is 4-5% for values near the lower reference limit. Total analysis time for an amniotic fluid sample is about 2 h.

Amniotic Fluid↗
PubMed1981

The composition of urinary stones analysed by infrared spectrophotometry and the precipitation of calcium phosphates from saturated solutions.

Of 205 consecutively analysed urinary stones, two-thirds were from men, which reflected the male: female ratio of urolithiasis cases at this hospital in the period of the study. Phosphate was found in 69.9% of the stones from male patients and in 94.2% of those from female patients. The phosphate in the urinary stones consisted of hydroxylapatite, carboxylapatite and/or MgHN4PO4. CaHPO4 could be demonstrated in only six of the 205 stones. Oxalate was present in 59.6 and 39.1% of the stones from males and females, respectively. Calcium phosphates from saturated solutions precipitated mainly as hydroxylapatite at pH levels above 5.47. Only below this level did CaHPO4 become visible in infrared spectrophotometry analysis. The solubility of calcium phosphates greatly increased with lowering of the pH. Increasing the ionic activity of the saturated solution with sodium chloride increased the solubility of calcium phosphates only to a limited extent.

Adult↗
PubMed1980

Lineation of the osmotic fragility curve of erythrocytes.

Lineation of the osmotic fragility curve by the method of Detraglia et al (1974) may be performed in most samples from a hospital population. Using the lineation procedure, the osmotic fragility may be tested by only 2 solutions of known osmolarity without any great loss of precision or accuracy. The osmotic fragility curve may be described by 2 values: C50 = the concentration at which 50% of the erythrocytes are haemolyzed and C50--C80 = the decrease in concentration raising the fraction of haemolysis from 0.50 to 0.80.

Erythrocytes↗
PubMed1980

Turbidity as a cause of error in the L-alanine transferase (ALAT) method.

When performing the ALAT-determination as recommended by the Scandinavian Committee on Enzymes, turbidity will develop due to a low ionic strength of the buffer. At the end of the pre-incubation period, the turbidity may still be developing, increasing the absorbance and lowering the ALAT-activity by 0-5 U/l. Turbidity and the error introduced in the assay, can be eliminated by increasing the ionic strength of the buffer by addition of NaCl.

Absorption↗
PubMed1980

The vitamin D status of man at 70 degrees north.

The overall vitamin D status of man at 70 degrees north has been evaluated by measuring the total 25-hydroxy-vitamin D (25-OHD) in serum samples from seventeen healthy adults from September 1977 to September 1978. The lowest 25-OHD-concentration was found in March (mean 51.5 nmol/l, SD = 19.5). This was significantly lower than in July and September 1977 and 1978 (P less than or equal to 0.0006). Of particular interest was the highly significant rise in the 25-OHD-concentration from March to July, most probably reflecting sun-induced endogenous synthesis in the skin. Despite the sun being below the horizon for two of the winter months, the 25-OHD-concentration remained at a constant and fairly high level (50-55 nmol/l).

Adult↗
PubMed1980

Determination of serum-phosphate without deproteinization by ultraviolet spectrophotometry of the phosphomolybdic acid complex.

Phosphate may be determined in serum without deproteinization as the phosphomolybdic acid complex by UV-spectrophotometry. The rate of complex formation is dependent on the acidity and the concentration of molybdate. The photometric measurements should be performed at a longer wavelength than in maximum absorptivity of the phosphomolybdic acid complex to permit practical high concentrations of molybdate in the reagents. Citric acid will hinder the complex formation, but will not interfere with the stability of the phosphomolydbic acid. The phosphomolybdic complex is determined by adding citric acid to the samples before (blank) and after (test) the molybdate. The analytical procedure has been automated on a Greiner GSA II, Greiner Electronics, Switzerland.

Humans↗
PubMed1977

Reference values for fibrinogen concentration and inhibitors of fibrinolysis.

epsilon-Aminocaproic acid and tranexamic acid when added to whole blood will dissolve in the plasma and draw water from the cells in accordance with their concentration. When these compounds are added to whole blood in recommended amounts (10 mg/ml), the osmotic dilution of the plasma must be taken into consideration when concentrated solutions or dry substances are used.

Aminocaproic Acid↗
PubMed1976

The Tromsøo heart study. Methods and main results of the cross-sectional study.

The mortality from coronary heart disease (CHD) in Norway increased rapidly during 1951-70, the highest mortality rates as well as the most rapid increases being found in Northern Norway. Several surveys of CHD were then planned, one of them is reported here. All men, 20-49 years of age, living in the municipality of Tromsøo, Troms county, were called up for examination. In total, 6595 men, 74.4% of those invited, were examined. Cholesterol, triglyceride and Hb values, BP, body weight and height, the percentage of smokers, and cigarette consumption have been tabulated according to area, age, work schedule, health condition, physical activity and ethnic background. The results suggest that the relatively high mortality from CHD in Northern Norway is associated with high serum cholesterol concentrations as well as a relatively high prevalence of smoking. During the screening there were indications of changes in dietary habits in the municipality, presumably as a result of accompanying publicity.

Activity Cycles↗