Sarcoidosis and lymphoma.
Hodgkin's Disease may mimic sarcoidosis, as seen in this case report.
Adult↗
Biomedical subjects
K Tung
Publications and source records attributed to K Tung.
Immunologic basis of male infertility.
Explore the source record for details and available documents.
Animals↗
pH and protease control of acrosomal content stasis and release during the guinea pig sperm acrosome reaction.
The purpose of this study was to examine how trypsin inhibitors affect the guinea pig sperm acrosome reaction in vitro. Using spermatozoa pretreated with lysophosphatidyl choline, we found that both naturally occurring high molecular weight and the smaller synthetic trypsin inhibitor p-aminobenzamidine (PAB) delayed the onset of the acrosome reaction as monitored by light microscopy. Examination with electron microscopy revealed that acrosomal matrix dispersal rather than membrane fusion was affected. Despite the morphologic delay in acrosomal content release, PAB unexpectedly permitted 96% of soluble acrosomal antigen to be released into the supernatant. In addition, total acrosin release in the presence of PAB was 74% of control, with the vast majority as latent rather than active enzyme. A morphologically intact but membrane-free target of acrosomal matrix (AM), which is sensitive to trypsin inhibitor, was partially purified using Triton-x-100 at pH 5.2. AM remained morphologically stable at pH 5.2; however, shift up to pH 7 resulted in rapid dissolution within several minutes as monitored by light and electron microscopy and light scattering. Trypsin inhibitor prevented dispersion of AM at pH 7. The results suggest that, during the acrosome reaction, one distinct region of the acrosomal contents disperses after membrane vesiculation in a pH and trypsin inhibitor-insensitive fashion while a pH sensitive trypsin-like activity (acrosin?) disperses another discrete region of acrosomal matrix.
Acrosin↗
Identification by flow cytometry of human monocytes with fucose-binding lectin (FBL) from Lotus tetragonolobus seeds.
Fucose binding lectin (FBL) from Lotus tetragonolobus seeds has previously been shown by fluorescence microscopy to bind to human neutrophils. This study shows using highly sensitive flow cytometry that this lectin binds both to human peripheral blood neutrophils and monocytes but not to lymphocytes. This binding is blocked by the presence of free L-fucose and is reversible when neutrophils or monocytes stained with fluorescent FBL are subsequently incubated in 0.05 M L-fucose. Quantitative comparison of neutrophils and monocytes from the same individual show that neutrophils bind approximately 2.6 times more FBL than monocytes and that FBL binding is more efficiently reversed with neutrophils, as compared with monocytes, by L-fucose. Additional double-labeling studies of cells with FBL and the OKM1 monoclonal antibody, which identifies monocytes and granulocytes, show that all cells binding FBL also stain with the OKM1 monoclonal antibody. This study shows that qualitatively, FBL may be utilized as a human myeloid cell marker to differentiate peripheral blood monocytes and neutrophils from lymphocytes.
Antigens, Surface↗