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K Uda

Publications and source records attributed to K Uda.

At least 55 records · Page 3Linked to original sources
PubMed1989

On the cDNA's for two types of rat pancreatic secretory trypsin inhibitor.

Two types of cDNA, which code for the two types of rat pancreatic secretory trypsin inhibitors (PSTIs), were cloned and sequenced. Both predicted amino acid sequences consisting of 79 amino acids, with the secretion signal peptide consisting of 18 and 23 amino acids for PSTI-I and PSTI-II, respectively. The nucleotide sequences were 91% homologous between the two cDNAs, but 68% and 65% homologous, respectively, when compared with human PSTI cDNA. Northern blot analyses showed that PSTI-I is expressed in the pancreas, whereas PSTI-II is expressed in the pancreas and the liver using the same promoter. Southern blot analyses suggested that both PSTI-I and PSTI-II genes are single copy genes per haploid genome. Duplication of rat PSTI gene seems to have occurred recently, after the divergence of humans and rats.

Amino Acid Sequence↗
PubMed1989

Rat epidermal growth factors: purification and tissue content.

Rat epidermal growth factor (rEGF) was isolated from the submaxillary gland of male rat by reversed phase high performance liquid chromatography and ion exchange chromatography. The binding of purified rEGF to human carcinoma cells (A-431) and its tritiated thymidine uptake on rat epidermal fibroblast cells (FR) were almost the same as those of purified commercially available mouse EGF (mEGF). Antisera to rEGF was raised in rabbits and a radioimmunoassay (RIA) system was established. The assay range of the RIA was about 1.0 to 100 ng/ml. The within assay coefficients of variation were 5 to 10%, while the between assay coefficients of variation were 5 to 13%. The tissue content of rEGF of male rats (10 weeks old) was examined. As a result, the submaxillary gland was found to contain a very high concentration of rEGF (214 micrograms/g wet tissue) as predicted, and digestive tissues, stomach, intestine and duodenum contained 2.49, 3.57, 9.44 ng/g wet tissue, respectively. The amounts in prostate and seminal vesicle were relatively high, being 65.6 and 2,268 ng/g wet tissue, respectively. The amount in the submaxillary gland increased markedly after 7 weeks of age. These results suggest that EGF is an important factor in gonadal function.

Aging↗
PubMed1989

Differences in stimulatory effects between rat pancreatic secretory trypsin inhibitor-61 and -56 on rat pancreas.

Two types of pancreatic secretory trypsin inhibitors (PSTIs) were recently purified from rat pancreatic juice. One consisted of 61 (PSTI-61) and the other of 56 (PSTI-56) amino acid residues. PSTI-61 has been reported to elicit cholecystokinin (CCK) release when injected into the duodenum. Since no information has been available about the action of PSTI-56 on CCK release, the two PSTIs were compared for their stimulatory effect on CCK release and pancreatic exocrine secretions in conscious rats after intraduodenal administration. Rats were prepared with bile and pancreatic fistulae and with two duodenal cannulae. Pancreatic juice was excluded from the duodenum for 48 h prior to the experiment because rat PSTIs were trypsin sensitive. PSTI-61 significantly stimulated pancreatic secretions and increased plasma CCK concentrations from 3.6 to 6.5 pM, whereas PSTI-56 had no effect on either CCK release or pancreatic secretions. It is suggested that the action as a regulator for CCK release and pancreatic secretions is possessed only by PSTI-61, but not by PSTI-56.

Amino Acid Sequence↗
PubMed1988

Release of pancreatic secretory trypsin inhibitor from human hepatoblastoma cells on stimulation with cytokines.

To determine whether or not human pancreatic secretory trypsin inhibitor (PSTI) is an acute phase reactant released from hepatocytes, we investigated the effects of various cytokines on the release of PSTI from cultured human hepatoblastoma cells. PSTI was synthesized in human hepatoblastoma cells and released on stimulation with various cytokines: interleukin-1, tumor necrosis factor and interferon-beta.

Biological Factors↗
PubMed1988

Elevation of serum pancreatic secretory trypsin inhibitor following serious injury.

Twenty-six of 31 seriously injured patients (84%) showed a marked elevation of serum pancreatic secretory trypsin inhibitor (PSTI) to more than twice the initial level within the first 2 weeks after admission. Serum PSTI rose from the second or third post-traumatic day and reached the maximum at day 5.8 on average. In uneventful cases, it returned to the level on admission within 2 weeks. The maximum serum PSTI in these patients was significantly correlated with the severity of the injury as judged at the time of admission, indicating that the elevation of serum PSTI in these patients was related to the extent of initial damage. In contrast, serum PSTI in patients with serious complications remained at high level even at 2 weeks after trauma, and it was not correlated with the initial severity of the injury.

Adult↗
PubMed1988

Purification, characterization and amino-acid sequencing of two pancreatic secretory trypsin inhibitors in rat pancreatic juice.

We purified two pancreatic secretory trypsin inhibitors (PSTI-I and PSTI-II) from rat pancreatic juice. PSTI-I consisted of 61 and PSTI-II of 56 amino-acid residues. Their amino-acid sequences were similar (40 out of 56 amino acid residues of PSTI-II being identical with those of PSTI-I), but PSTI-I and PSTI-II appeared to be translation products of different genes. There was no difference in inhibitory properties between PSTI-I and PSTI-II.

Amino Acid Sequence↗
PubMed1987

Distribution of pancreatic secretory trypsin inhibitor in various human tissues and its inactivation in the gastric mucosa.

Considerable amounts of immunoreactive pancreatic secretory trypsin inhibitor were detected in lung, stomach, liver, pancreas, kidney, small intestine and ovary. Immunoreactive pancreatic secretory trypsin inhibitor content of surgically removed gastric mucosa was approximately seven times higher than that of gastric mucosa obtained at autopsy. The reduction in PSTI-immunoreactivity seen in a tissue extract of fresh gastric mucosa on 24 hours' incubation represented 9% of the original immunoreactivity in 10 mM Tris-HCl buffer, pH 7.4, at 37 degrees C. While, there was a more than 80% reduction in immunoreactivity on 12 hours' incubation at 37 degrees C at pH 4.0 or under more acidic conditions.

Female↗
PubMed1986

Vasoactive intestinal peptide- and peptide histidine isoleucine amide-like immunoreactivity colocalize with vasopressin-like immunoreactivity in the canine hypothalamo-neurohypophysial neuronal system.

The distribution of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine amide (PHI) was investigated in the canine hypothalamus by immunocytochemistry. VIP- and PHI-like immunoreactive neurons were detected in the magnocellular supraoptic and paraventricular nucleus. These magnocellular VIP- and PHI-producing neurons coexist with vasopressin-like immunoreactivity and send axons to the median eminence and neurohypophysis. These findings may serve as an anatomical basis for studying the function of VIP and PHI on pituitary hormone secretion.

Animals↗
PubMed1986

Sexual differences in the distribution of substance P immunoreactive fibers in the ventral horn of the rat lumbar spinal cord.

The distribution of substance P (SP) in the rat spinal cord was investigated by peroxidase-anti-peroxidase immunocytochemistry combined with retrograde horseradish peroxidase (HRP) labeling via the cremaster muscle. In the male rats, a dense network of SP immunoreactive (SP-IR) fibers and terminals was detected in the ventral column of the L1 and L2 segments (Vent L1-2) with a different density and extent from the other segmental levels. These fibers and terminals were accumulated within and around the nucleus centromedialis lumbaris (CM) of the L1 and L2 segments. However, in the female rats, SP-IR fibers and terminals were sparse in the Vent L1-2 without particular segmental differences. HRP-positive motoneurons were located in the CM and surrounded by SP-IR fibers and terminals. These results indicate that the Vent L1-2 of the rat spinal cord shows sexual dimorphism with respect to the regional distribution of SP-IR fibers and terminals, and that motoneurons that innervate the cremaster muscle are innervated by dense SP-IR fibers and terminals.

Animals↗
PubMed1985

Distribution of human leumorphin-like immunoreactivity in the monkey spinal cord revealed by immunocytochemistry.

The regional distribution of human leumorphin (HL)-like immunoreactivity (HL-LI) in monkey (Macaca fuscata) spinal cord and dorsal root ganglia was investigated by peroxidase-anti-peroxidase immunocytochemistry using specific antiserum. HL-LI-positive fibers and terminals were distributed densely in laminae (Rexed) I and II, and sparsely in laminae III-VII and X, but no immunoreactive elements were observed in the ventral horn, the white matter or the dorsal root ganglia. Many immunoreactive neuronal perikarya were found in laminae I and II. Intrathecal injection of colchicine also revealed the presence of immunoreactive neuronal perikarya in laminae III-VII and X. These results suggest the presence of HL-LI, which represents HL and/or its C-terminal fragment, in the neuronal elements of the monkey spinal cord.

Animals↗
PubMed1985

Immunocytochemical localization of substance P in the rat spinal cord with special reference to fibers within the ventral column of the rostral lumbar segments.

The distribution of substance P (SP) in the rat spinal cord was investigated by peroxidase-anti-peroxidase (PAP) immunocytochemistry. A dense network of SP-immunoreactive fibers and terminals was detected in the ventral column of the rostral lumbar cord with a different density and extent from the other segmental levels. These fibers and terminals were accumulated within and around the centromedial nucleus (CM) of the L1 and L2 segments, with some bundles of immunoreactive fibers between the CM and other areas; i.e. laminae V and X and the contralateral CM. They formed a dense network, such as in arborization of immunoreactive fibers and terminals on the transverse plane and in a comb-shaped structure on the horizontal plane. The origin of the SP in this network was examined. It was determined that neither a total transection of spinal cord at a low thoracic level or mid-lumbar level, nor at an ipsilateral or bilateral section of the 3-5 dorsal roots, containing L1 and L2 roots, induced any visible changes in the SP staining pattern. An intrathecal injection of colchicine revealed the presence of SP-immunoreactive neurons in the dorsal horn and intermediate gray matter at the spinal cord including the rostral lumbar cord. The present findings suggested that the majority of SP immunoreactivities in the above network are derived from local circuit interneurons of the spinal cord.

Animals↗
PubMed1985

High-performance liquid chromatographic determination of N1-alkylnicotinamide in urine.

A simple high-performance liquid chromatographic method has been developed for determining N1-alkylnicotinamides, including C1-C5 alkyl derivatives, in urine. N1-Alkylnicotinamides were reacted with acetophenone in strong alkali medium at 0 degrees C and then formic acid was added. The reaction mixture was heated in acidic medium at above 93 degrees C, and the fluorescent product, 1-alkyl-7-phenyl-1,5-dihydro-5-oxo-1,6-naphthyridine, was chromatographed by HPLC, using a Zorbax SCX-300 column with a mixed mobile phase of acetonitrile-0.04 M ammonium phosphate, monobasic. N1-Alkylnicotinamides can be determined as 1,6-naphthyridine derivatives by a fluorometric detector at a level of 100 pg (signal/noise = 2). Recoveries of N1-alkylnicotinamides in urine were satisfactory. Interfering reaction products from NAD+ and NADP+ were clearly eliminated for determination of N1-alkylnicotinamides without pentyl derivatives.

Adult↗
PubMed1980

[Pharmacological studies of new sulfhydryl compounds 2-mercapto-2-methylpropanoyl-L-cysteine (SA96). I. Evaluation of anti-rheumatic action (author's transl)].

Oral administration of 2-mercapto-2-methylpropanoyl-L-cysteine (SA 96), a newly synthesized sulfhydryl compound, showed protective and curative effects on adjuvant-induced arthritis in rats similarly to those seen with D-penicillamine (D-PA). However, the effects of these compounds were not dose-dependent, and the maximum effects of SA96 were observed at 10 mg/kg/day. On the contrary, SA96 and D-PA had little effect on the various acute and subacute inflammatory responses induced in rat and mice. Formation of hemolytic plaque forming cells in the spleen of mice immunized with 5 X 10(8) sheep red blood cells was potentiated by the oral administration of both compounds. These stimulatory effects of SA96 and D-PA on the humoral immune responses were also not dose-dependent, and the maximum effects of SA96 were observed with 10 mg/kg/day, as in the case of adjuvant-induced arthritis in rats. In in vitro experiments, the inactivation of rheumatoid factor and the inhibition of collagenase and bone alkaline phosphatase activities were observed with both compounds, but these effects of SA96 were more potent than those of D-PA. As there is a similarity in the pharmacological profiles of SA96 and D-PA, SA96 may prove to be clinically effective for rheumatoid arthritis.

Acute Disease↗
PubMed1980

Antianaphylactic effects of dipivalyl epinephrine and related compounds in rat conjunctiva.

Topically applied dipivalyl epinephrine (DPE) and related compounds have been found to inhibit passive anaphylactic reaction in rat conjunctiva. The order of activity is as follows: isoproterenol greater than DPE greater than epinephrine greater than norepinephrine. The antianaphylactic effect of DPE was antagonized by propranolol but was not affected by phentolamine. The effects of epinephrine, norepinephrine, and isoproterenol were also antagonized by propranolol but potentiated by pentolamine. From these findings, it was suggested that DPE not only exerts its antianaphylactic action through activation of beta-adrenergic receptor but also itself has a little different action from epinephrine.

Anaphylaxis↗
PubMed1979

[The role of lysosomal enzymes in adjuvant-induced uveitis in rabbits (author's transl)].

Acid phosphatase and beta-glucuronidase were used as marker enzymes of lysosome, and their role in adjuvant-induced uveitis was studied. These enzyme activities in the iris-ciliary processes were increased in the inflamed tissues. Changes in these enzyme activities in the tissues paralleled the development of uveitis. While protein concentration in the aqueous humor as a parameter of vascular permeability was significantly correlated with these enzyme activities, there was no correlation in the leucocyte counts in the aqueous humor. Topically applied dexamethasone reduced the increase in the aqueous protein, the leucocyte migration and the swelling of the iris-ciliary processes, while topically applied indomethacin reduced only the leucocyte migration among these inflammatory parameters. Acid phosphatase activities in the inflamed tissues were reduced also by dexamethasone, but not by indomethacin.

Acid Phosphatase↗