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K Underwood

Publications and source records attributed to K Underwood.

8 recordsLinked to original sources

Relationship of N-acetyl-beta- glucosaminidase levels to seriousness of suicide attempts.

We hypothesized that levels of N-acetyl-beta-glucosaminidase (NAG) would differ between patients making serious versus nonserious suicide attempts. The levels of NAG from 11 patients hospitalized for serious suicide attempts were compared to 5 patients whose suicide attempts were not rated serious. There was a significant difference in the mean NAG levels of the serious suicide attempters and the nonserious attempters (23 versus 52; p = 0.01). NAG may be a marker for a biological variable that is associated with the seriousness of a suicide attempt.

Acetylglucosaminidase↗

An encyclopedia of mouse genes.

The laboratory mouse is the premier model system for studies of mammalian development due to the powerful classical genetic analysis possible (see also the Jackson Laboratory web site, http://www.jax.org/) and the ever-expanding collection of molecular tools. To enhance the utility of the mouse system, we initiated a program to generate a large database of expressed sequence tags (ESTs) that can provide rapid access to genes. Of particular significance was the possibility that cDNA libraries could be prepared from very early stages of development, a situation unrealized in human EST projects. We report here the development of a comprehensive database of ESTs for the mouse. The project, initiated in March 1996, has focused on 5' end sequences from directionally cloned, oligo-dT primed cDNA libraries. As of 23 October 1998, 352,040 sequences had been generated, annotated and deposited in dbEST, where they comprised 93% of the total ESTs available for mouse. EST data are versatile and have been applied to gene identification, comparative sequence analysis, comparative gene mapping and candidate disease gene identification, genome sequence annotation, microarray development and the development of gene-based map resources.

Animals↗

Association of N-acetyl-beta-glucosaminidase levels with seriousness of suicide attempts.

We hypothesized that levels of N-acetyl-beta-glucosaminidase (NAG) would differ between patients making serious versus nonserious suicide attempts. The levels of NAG from 7 patients hospitalized for serious suicide attempts were compared to 3 patients whose suicide attempts were not rated serious. There was a significant difference in the mean NAG levels of the serious suicide attempters and the nonserious attempters, 25 versus 61; p = .036. Implications of this finding are discussed.

Acetylglucosaminidase↗

Generation and analysis of 280,000 human expressed sequence tags.

We report the generation of 319,311 single-pass sequencing reactions (known as expressed sequence tags, or ESTs) obtained from the 5' and 3' ends of 194,031 human cDNA clones. Our goal has been to obtain tag sequences from many different genes and to deposit these in the publicly accessible Data Base for Expressed Sequence Tags. Highly efficient automatic screening of the data allows deposition of the annotated sequences without delay. Sequences have been generated from 26 oligo(dT) primed directionally cloned libraries, of which 18 were normalized. The libraries were constructed using mRNA isolated from 17 different tissues representing three developmental states. Comparisons of a subset of our data with nonredundant human mRNA and protein data bases show that the ESTs represent many known sequences and contain many that are novel. Analysis of protein families using Hidden Markov Models confirms this observation and supports the contention that although normalization reduces significantly the relative abundance of redundant cDNA clones, it does not result in the complete removal of members of gene families.

Adult↗

Serum antibody opsonic activity against Actinobacillus actinomycetemcomitans in human periodontal diseases.

Actinobacillus actinomycetemcomitans is frequently associated with severe periodontitis. Many periodontitis patients have elevated levels of serum IgG antibodies to A. actinomycetemcomitans, but the role of these antibodies is unknown. This study evaluated the functional capacity of anti-A. actinomycetemcomitans IgG antibody to enhance phagocytosis of A. actinomycetemcomitans by polymorphonuclear leukocytes. Chemoluminescence assays were done using sera from 64 subjects, 61 of whom had severe periodontitis; results were compared with the subject's anti-A. actinomycetemcomitans IgG titer and avidity. There was a strong correlation between chemoluminescence and antibody log titer (P < .00001) and a weak correlation between chemoluminescence and antibody avidity (P < .05). The results support the hypothesis that anti-A. actinomycetemcomitans IgG antibodies are important in promoting phagocytosis and killing of A. actinomycetemcomitans. Subjects who develop high levels of highly avid antibodies against A. actinomycetemcomitans may have greater resistance to continued or repeated infection by this pathogen.

Actinobacillus Infections↗

Bully/victim problems among middle school children.

Bully/victim problems among six classes of 8-9 year-old and six classes of 11-12 year-old children, attending three middle schools, were investigated by means of Olweus's self-report Bullying Inventory. About 21 per cent of the children reported being bullied, and about 17 per cent reported bullying others, "sometimes" or more often. Reports of both bullying and being bullied were more prevalent among boys than among girls, and among the younger group than among the older group. The two most common forms of bullying were reported to be teasing and hitting/kicking. Fewer of the younger group than the older group reported being bullied by same-age pupils, and more of the former reported being bullied by older pupils. Most boys were bullied by other boys only, whereas girls were more likely to be bullied by children of either sex. Besides being bullied in school, children also reported that this happened on the journey to/from school and in other places such as in the street near where they live. Many children expressed negative attitudes towards bullying, although nearly a third said that they could understand why it happens. The majority of the children who reported being bullied/bullying others had not been spoken to about this by teachers or by someone at home. Victims of bullying, but not bullies, were found to be most likely to report feeling unhappy and lonely at school, and to report having fewer good friends. In Study 2, children identified as bullies, victims and not involved in this type of problem were interviewed to find out why certain children and/or themselves bully/get bullied by others, and the feelings of the children involved. The pattern of responses by the three groups differed in some important ways.

Aggression↗

Nesiritide use for critically ill children awaiting cardiac transplantation.

BACKGROUND: This study aimed to explore the effects of nesiritide in children awaiting cardiac transplantation. METHODS: At the discretion of the attending physician, nesiritide was added to standard therapy for seven children (median age, 11 months) and continued until clinical improvement or transplantation. Blood pressure and urine output data were compared with data from the day before nesiritide institution. Safety parameters were assessed throughout the study. RESULTS: Urine output increased from 2.6 to 3.6 ml/kg/h (p = 0.04). No significant blood pressure changes or adverse effects related to nesiritide were noted. CONCLUSION: Nesiritide may be safe and effective for children with severe heart failure.

Blood Pressure↗