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Biomedical subjects

K Urbańczyk

Publications and source records attributed to K Urbańczyk.

14 recordsLinked to original sources
PubMed1999

Preferential overexpression of CD44v5 in advanced gastric carcinoma Goseki grades I and III.

Tumor progression is associated with the clonal expansion of surviving cell variants. These results in cancer cell heterogeneity and selection of cells with a high malignant potential reflected also by the ability to metastasize. In seeding and implantation of cancer cells at the distant site cell adhesion molecules play a crucial role. Of particular interest is CD44 adhesion molecule, which possibly is involved in tumor metastasis development. Forty cases of an advanced gastric cancer were studied. Paraffin block were collected from the files. In addition to routine tumor typing, grading (Lauren and Goseki classifications) and staging, CD44 (standard, v5 and v6) was studied by immunohistochemistry and RT-PCR. CD44 immunoreactivity was found in 36 of the 40 studied cases. A significant overexpression of CD44v5 was noticed in gastric cancer. This was especially seen in Goseki's grades I and III (72.7% of cases) and was less common in Goseki's grades II and IV (44.4% of cases). CD44v6 was less commonly expressed. In some cases CD44 heterogeneity of neoplastic intravascular emboli was noticed and in some other cases stronger expression of CD44 was present in deeper parts of cancer infiltrate. Immunohistochemical expression was mostly in concert with CD44 gene expression as shown by RT-PCR results. Some discrepancies are discussed. These findings are interesting in view of better prognosis and different route of dissemination of Goseki's grades I + III compared with Goseki's grades II + IV of the gastric cancer. We have shown an overexpression of CD44v5 in an advanced gastric cancer, especially in Goseki's grades I and III. This could reflect a different malignant potential and a different route of dissemination of gastric well differentiated adenocarcinomas.

Adenocarcinoma↗
PubMed1999

Adenocarcinoma developes in the "cirrhotic" not just atrophic gastric mucosa.

Helicobacter pylori has been postulated as a group 1 carcinogen, but the mechanism of gastric carcinogenesis remains to be elucidated. It is believed that gastric cancer morphogenesis follows Correa's paradigm of a gastritis-->atrophy-->metaplasia-->dysplasia-->cancer sequence. The aim of the present study was to investigate by histology non-cancerous gastric mucosa in early gastric cancer. Forty cases of early gastric cancer were collected from the files. In all cases of well differentiated adenocarcinoma mucosal atrophy and intestinal metaplasia were present in non-cancerous gastric mucosa. In addition, focal glandular hyperplasia was present in 83.3% of cases and hyperplastic surface epithelium was present in 50% of cases. Mucosal atrophy and intestinal metaplasia were seen in 90.5% of poorly differentiated adenocarcinomas. This was accompanied by surface epithelium hyperplasia in 66.7% and glandular hyperplasia in 47.6% of cases. In a diffuse early gastric cancer atrophy was present in 71.4%, intestinal metaplasia in 57.1%, while hyperplasia (either surface epithelium or glandular) was present in 42.8%. With the introduction of Genta's "strict definition" of gastric mucosal atrophy it became clear that atrophic gastric mucosa is not just a case of simple atrophy but can be in some respect compared to a cirrhotic organ.

Adenocarcinoma↗
PubMed1998

Lauren's and Goseki's grade distribution does not change along with the progression from an early to advanced gastric carcinoma.

It is generally believed that tumor progression is associated with increased cancer cell heterogeneity, poorer differentiation and increased malignant potential. We tested this hypothesis by comparing a group of early and advanced gastric carcinomas. We retrieved from our files 40 cases of early gastric carcinoma and another 40 cases of advanced gastric carcinoma. Routine tumor typing, grading (Lauren and Goseki classifications) and staging was applied. We have shown that the proportion of histological grades in early and advanced gastric carcinoma does not change significantly. In addition, more poorly differentiated carcinomas were present both in early and advanced gastric carcinoma groups. This is in contrast with several other studies. This fact together with overexpression of CD44V5 isoform and fewer cancer cells circulating in the blood of patients suffering from this type of gastric carcinoma might influence the adjuvant therapy strategy. In summary, in the present study we noticed a similar distribution of Lauren's and Goseki's histological grades both in early and advanced gastric carcinoma.

Adult↗
PubMed1996

Pregnancy associated diffuse malignant fibrous mesothelioma of peritoneum. Immunohistochemical studies of ectopic decidual reaction and concomitant myofibroblastic and mesothelial proliferations.

A case of diffuse proliferations in the peritoneum in a 24-year-old woman several months post partum has been reported. Multinodular growth of myofibroblastic cells without atypia was observed, which predominated and revealed a positive immunohistochemical reaction to muscle-specific actin and vimentin, and dispersed clusters of mesothelial cells showing a positive reaction to cytokeratin and vimentin, with moderate atypia and scarce mitotic figures. On cesarean section the greater omentum contained an ectopic decidua in the form of a nodule. Decidual cells, in part transforming into spindle-shaped cells, showed the presence of desmin. Multifocal proliferation of myofibroblasts, most probably of metaplastic type, concomitant with pregnancy-induced peritoneal deciduosis and diffuse progressive mesothelial proliferations has been identified.

Adult↗
PubMed1995

Pancreatic metaplasia of the human gastric mucosa is associated with high expression of transforming growth factor alpha but not of epidermal growth factor.

Pancreatic acinar metaplasia of the gastric mucosa is a newly recognized entity. Its physiological relevance and association with other pathological conditions in the stomach remain to be elucidated. We studied by immunohistochemistry the expression of growth markers in the gastric mucosa in biopsies from 15 patients with recognized pancreatic metaplasia. Pancreatic metaplasia (both acinar and dispersed forms) was found in routine paraffin sections and confirmed by strong lipase immunoreactivity. In parallel paraffin sections we performed immunostaining for epidermal growth factor (EGF), transforming growth factor-alpha (TGF alpha) and epidermal growth factor receptor (EGFr) using a biotin streptavidin method. Strong expression of TGF alpha but only weak expression of EGF was noted within metaplastic mucosa. EGFr was strongly expressed, not only in areas of pancreatic metaplasia but also in the surrounding gastric mucosa.

Antibodies↗
PubMed1994

Dispersed and acinar forms of pancreatic metaplasia in human gastric mucosa.

Pancreatic acinar cell metaplasia of the gastric mucosa is a newly recognized entity. Its physiological relevance and association with other gastric pathological conditions remains to be elucidated. We studied morphology of pancreatic metaplasia of the gastric mucosa in 15 cases. Two morphological forms of pancreatic metaplasia were noticed: an acinar form and a diffuse form. The diffuse form is characterized by small foci of pancreatic metaplasia dispersed in the gastric mucosa without any separation from the surrounding gastric glands. The acinar form is characterized by acini of pancreatic metaplasia separated from the surrounding gastric mucosa by strands of connective tissue and single smooth muscle cells. Acinar form of pancreatic metaplasia seems to be spared by the chronic atrophic gastritis involving the neighboring gastric mucosa.

Adult↗
PubMed1991

Selected immunohistochemical markers in stromal neoplasms of the gastrointestinal tract.

We studied immunohistochemical reactions to vimentin, desmin and protein S-100 in 43 cases of stromal tumors of the gastrointestinal tract. The material studied included: 1 esophageal tumor, 18 gastric tumors, 19 small intestinal tumors and 5 colonic tumors, classified in 13 cases as benign and in 30 cases as malignant neoplasms of various degree of malignancy. Mean age of the patients was 58.9 years. A positive reaction to vimentin was found in 37 cases, a negative reaction concerned an esophageal tumor, two benign tumors (gastric and small intestinal) and three malignant tumors (gastric and two small intestinal). A positive reaction to desmin was detected in an esophageal tumor and in nine gastric tumors. Only one benign small intestinal tumor and three benign colonic tumors showed a positive reaction to desmin. Protein S-100 was found in an esophageal tumor and in 7 out of 18 gastric tumors and in 12 out of 24 intestinal tumors. Coexpression of vimentin and desmin was found in 8 gastric tumors, only in one small intestinal tumor and in three colonic tumors. Three gastric tumors showing both these reactions were all benign. Coexpression of desmin and protein S-100 was found in 7 out of 43 tumors of the alimentary tract. In six cases these tumors were benign. Basing on the results we may say that the presence of these antigens reflects the degree of differentiation of gastrointestinal stromal tumors of the gastrointestinal tract, though it does not allow to choose unequivocally conclusions as to their histogenesis.

Adult↗
PubMed1991

Gastric antral vascular ectasia. A case report.

A case of vascular lesion of angiodysplastic type in the pyloric part of the stomach has been reported in a patient with chronic viral hepatitis. The diagnosis was made from clinical symptoms, endoscopic and histopathologic findings in the specimens obtained during gastroscopy. Many authors emphasize a correlation between the incidence of "gastric antral vascular ectasia" and liver diseases, especially cirrhosis with portal hypertension. A review of bibliography with particular consideration of its etiology has been presented.

Angiodysplasia↗
PubMed1990

[Immunoreactivity directed towards human papillomavirus (HPV) structural antigen in perianal epithelial proliferation in humans].

Immunoreactivity directed towards the presence of a structural antigen of Papilloma virus (HPV) was studied in patients with benign epithelial proliferations in the perianal area, squamous cell and cloacogenic carcinomas of the rectum and anus, using Dako serum kits. Positive reaction was found in five of eleven pointed condylomas and in one squamous cell papilloma, which is in accordance with other reports. Negative reaction in patients with squamous cell carcinomas may result from methodologic limitations of immunohistochemical studies. In patients with cloacogenic carcinomas it may also indicate a lack of relationship between this type of a neoplasm and HPV infection. The latter probably requires the presence of mature keratocytes to proliferate.

Aged↗
PubMed1990

[Alveolar soft part sarcoma--case report].

A case of alveolar soft part sarcoma has been described in a 12-year from old girl. Immunohistochemical reactions on paraffin sections from the tumour were directed towards the presence of desmin , vimentin and neurospecific enolase. Positive reaction to desmin and neurospecific enolase was found in neoplastic cells.

Child↗
PubMed1989

Coexpression of vimentin and desmin in gastric leiomyomas. An immunohistochemical study in paraffin sections.

The aim of the study was to evaluate the incidence of the reactions for vimentin and desmin in gastric leiomyomas routinely processed in formalin and embedded i paraffin. The material studied included four benign leiomyomas, seven malignant leiomyomas and three malignant epithelioid leiomyomas. A positive reaction to vimentin was found in 13 out 14 leiomyomas under study. The number of neoplastic cells showing vimentin expression was larger in malignant, especially epithelioid leiomyomas than in non-malignant leiomyomas. A positive reaction for desmin in neoplastic cells was found in 9 leiomyomas 64%. One non-malignant leiomyoma showed a moderate reaction. In the remaining eight cases the reaction was weak and occurred in single neoplastic cells. Coexpression of vimentin and desmin in neoplastic cells occurred in 8 out of 9 leiomyomas with a positive reaction for desmin. Coexpression of vimentin and desmin occurred also in the smooth muscle cells of blood vessels in all 14 cases. A weak reaction for desmin or its lack in the tumour cells of leiomyomas with its marked expression in the smooth muscle cells of the blood vessels and gastric wall outside the tumor points rather to a small number of desmin filaments in the neoplastic cells than to their destruction by fixation in formalin. The occurrence of the reaction to desmin only in a limited number of neoplastic cells questions the reliability of its use in the oligopiopsy material.

Desmin↗